Molluscan Beclin-1 is involved in the innate immune response by regulating the autophagosomes formation in Crassostrea hongkongensis
Beclin-1 plays a central role in autophagy, which is well documented in mammals. However, the identity and function of Beclin-1 remain largely unclear in mollusks. In this study, a Beclin-1 gene (ChBeclin-1) was identified and characterized from Crassostrea hongkongensis. The full length of ChBeclin...
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ftdoajarticles:oai:doaj.org/article:b390f71e3d084a12bb9acc258852bda9 2023-05-15T15:58:55+02:00 Molluscan Beclin-1 is involved in the innate immune response by regulating the autophagosomes formation in Crassostrea hongkongensis Yinyin Zhou Kunna Liu Xingyou Li Yanpin Qin Yuehuan Zhang Yang Zhang Zhiming Xiang Haitao Ma Jun Li Ziniu Yu 2020-11-01T00:00:00Z https://doi.org/10.1016/j.aqrep.2020.100541 https://doaj.org/article/b390f71e3d084a12bb9acc258852bda9 EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S2352513420306347 https://doaj.org/toc/2352-5134 2352-5134 doi:10.1016/j.aqrep.2020.100541 https://doaj.org/article/b390f71e3d084a12bb9acc258852bda9 Aquaculture Reports, Vol 18, Iss , Pp 100541- (2020) Beclin-1 Autophagy pathway Crassostrea hongkongensis Innate immune Aquaculture. Fisheries. Angling SH1-691 article 2020 ftdoajarticles https://doi.org/10.1016/j.aqrep.2020.100541 2022-12-31T08:14:26Z Beclin-1 plays a central role in autophagy, which is well documented in mammals. However, the identity and function of Beclin-1 remain largely unclear in mollusks. In this study, a Beclin-1 gene (ChBeclin-1) was identified and characterized from Crassostrea hongkongensis. The full length of ChBeclin-1 cDNA encoded a predicted 442-amino acid protein with a deduced molecular weight of 51.11 kDa and a theoretical isoelectric point of 4.71. The putative amino acid sequence of ChBeclin-1 contained a typical Bcl-2 homology domain 3 (BH3), a coiled-coil domain (CCD), and an Atg6 domain. Phylogenetic analysis showed that ChBeclin-1 was clustered with Beclin-1 from other mollusks such as Crassostrea gigas, Crassostrea virginica, and Mizuhopecten yessoensis. ChBeclin-1 mRNA was constitutively expressed in all detected tissues, with the highest relative expression in the gills. Additionally, significant inductions in the mRNA level of ChBeclin-1 were observed after bacterial stimulations, indicating its involvement in the innate immune response. The knockdown of ChBeclin-1 via RNA interference impeded the formation of autophagosomes, denoting ChBeclin-1 plays a role in autophagy. Moreover, in subcellular localization and dual-luciferase reporter assays, ChBeclin-1 protein was found to be localized mainly in the endoplasmic reticulum, and its overexpression suppressed the transcriptional activity of an NF-κB reporter gene in a dose-dependent manner in HEK 293T cells. Collectively, these findings provide experimental evidence of a functional Beclin-1 in autophagy and demonstrate its involvement in the innate immunity of C. hongkongensis. Article in Journal/Newspaper Crassostrea gigas Directory of Open Access Journals: DOAJ Articles Aquaculture Reports 18 100541 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Beclin-1 Autophagy pathway Crassostrea hongkongensis Innate immune Aquaculture. Fisheries. Angling SH1-691 |
spellingShingle |
Beclin-1 Autophagy pathway Crassostrea hongkongensis Innate immune Aquaculture. Fisheries. Angling SH1-691 Yinyin Zhou Kunna Liu Xingyou Li Yanpin Qin Yuehuan Zhang Yang Zhang Zhiming Xiang Haitao Ma Jun Li Ziniu Yu Molluscan Beclin-1 is involved in the innate immune response by regulating the autophagosomes formation in Crassostrea hongkongensis |
topic_facet |
Beclin-1 Autophagy pathway Crassostrea hongkongensis Innate immune Aquaculture. Fisheries. Angling SH1-691 |
description |
Beclin-1 plays a central role in autophagy, which is well documented in mammals. However, the identity and function of Beclin-1 remain largely unclear in mollusks. In this study, a Beclin-1 gene (ChBeclin-1) was identified and characterized from Crassostrea hongkongensis. The full length of ChBeclin-1 cDNA encoded a predicted 442-amino acid protein with a deduced molecular weight of 51.11 kDa and a theoretical isoelectric point of 4.71. The putative amino acid sequence of ChBeclin-1 contained a typical Bcl-2 homology domain 3 (BH3), a coiled-coil domain (CCD), and an Atg6 domain. Phylogenetic analysis showed that ChBeclin-1 was clustered with Beclin-1 from other mollusks such as Crassostrea gigas, Crassostrea virginica, and Mizuhopecten yessoensis. ChBeclin-1 mRNA was constitutively expressed in all detected tissues, with the highest relative expression in the gills. Additionally, significant inductions in the mRNA level of ChBeclin-1 were observed after bacterial stimulations, indicating its involvement in the innate immune response. The knockdown of ChBeclin-1 via RNA interference impeded the formation of autophagosomes, denoting ChBeclin-1 plays a role in autophagy. Moreover, in subcellular localization and dual-luciferase reporter assays, ChBeclin-1 protein was found to be localized mainly in the endoplasmic reticulum, and its overexpression suppressed the transcriptional activity of an NF-κB reporter gene in a dose-dependent manner in HEK 293T cells. Collectively, these findings provide experimental evidence of a functional Beclin-1 in autophagy and demonstrate its involvement in the innate immunity of C. hongkongensis. |
format |
Article in Journal/Newspaper |
author |
Yinyin Zhou Kunna Liu Xingyou Li Yanpin Qin Yuehuan Zhang Yang Zhang Zhiming Xiang Haitao Ma Jun Li Ziniu Yu |
author_facet |
Yinyin Zhou Kunna Liu Xingyou Li Yanpin Qin Yuehuan Zhang Yang Zhang Zhiming Xiang Haitao Ma Jun Li Ziniu Yu |
author_sort |
Yinyin Zhou |
title |
Molluscan Beclin-1 is involved in the innate immune response by regulating the autophagosomes formation in Crassostrea hongkongensis |
title_short |
Molluscan Beclin-1 is involved in the innate immune response by regulating the autophagosomes formation in Crassostrea hongkongensis |
title_full |
Molluscan Beclin-1 is involved in the innate immune response by regulating the autophagosomes formation in Crassostrea hongkongensis |
title_fullStr |
Molluscan Beclin-1 is involved in the innate immune response by regulating the autophagosomes formation in Crassostrea hongkongensis |
title_full_unstemmed |
Molluscan Beclin-1 is involved in the innate immune response by regulating the autophagosomes formation in Crassostrea hongkongensis |
title_sort |
molluscan beclin-1 is involved in the innate immune response by regulating the autophagosomes formation in crassostrea hongkongensis |
publisher |
Elsevier |
publishDate |
2020 |
url |
https://doi.org/10.1016/j.aqrep.2020.100541 https://doaj.org/article/b390f71e3d084a12bb9acc258852bda9 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Aquaculture Reports, Vol 18, Iss , Pp 100541- (2020) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2352513420306347 https://doaj.org/toc/2352-5134 2352-5134 doi:10.1016/j.aqrep.2020.100541 https://doaj.org/article/b390f71e3d084a12bb9acc258852bda9 |
op_doi |
https://doi.org/10.1016/j.aqrep.2020.100541 |
container_title |
Aquaculture Reports |
container_volume |
18 |
container_start_page |
100541 |
_version_ |
1766394693084512256 |