Anticancer activity of Cyanothece sp. strain extracts from Egypt: First record
Objective: To assess the anticancer activity of eight cyanobacterial hydrophilic extracts on Ehrlich ascites carcinoma cell line. Methods: The cyanobacterial strains used in the investigation were collected from diverse habitats in Egypt. The initial cytotoxicity test of cyanobacterial hydrophilic e...
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Wolters Kluwer Medknow Publications
2015
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ftdoajarticles:oai:doaj.org/article:b37f3f0139284d61981bb4f970c5b042 2023-05-15T15:07:07+02:00 Anticancer activity of Cyanothece sp. strain extracts from Egypt: First record Nermin Adel El Semary Manar Fouda 2015-12-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2015.09.004 https://doaj.org/article/b37f3f0139284d61981bb4f970c5b042 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115002166 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2015.09.004 https://doaj.org/article/b37f3f0139284d61981bb4f970c5b042 Asian Pacific Journal of Tropical Biomedicine, Vol 5, Iss 12, Pp 992-995 (2015) Anticancer Cell line Cyanothece Hydrophilic extract Molecular Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2015 ftdoajarticles https://doi.org/10.1016/j.apjtb.2015.09.004 2022-12-31T00:37:07Z Objective: To assess the anticancer activity of eight cyanobacterial hydrophilic extracts on Ehrlich ascites carcinoma cell line. Methods: The cyanobacterial strains used in the investigation were collected from diverse habitats in Egypt. The initial cytotoxicity test of cyanobacterial hydrophilic extracts was carried out by MTT assay. The in vitro anticancer activity of the four most active extracts was performed on MCF-7 cells using sulforhodamine B assay. Morphological and molecular techniques were used to characterise identity of the isolate from which the most potent cytotoxic extract was obtained. Results: Extracts from four cyanobacterial strains had higher cytotoxic activities scoring 76.68%, 77.70%, 76.70% and 74.45%, respectively. A considerable anticancer effect was only detected when the concentrated extracts were used. One cyanobacterial extract gave the highest anticancer activity on human breast adenocarcinoma cell line (57.6% of inhibition) as compared to control. The isolate was best-matched to Cyanothece sp. with sequence resemblance 98% to Cyanothece sp. strain PCC7564 and the phylogenetic analysis confirmed its close identity to the Cyanothece genus. Conclusions: This is the first study to report the anticancer effect of aqueous extracts derived from the unicellular Cyanothece sp. from Egypt and its potential as a plausible candidate for future mass biotechnological applications. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 5 12 992 995 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Anticancer Cell line Cyanothece Hydrophilic extract Molecular Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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Anticancer Cell line Cyanothece Hydrophilic extract Molecular Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Nermin Adel El Semary Manar Fouda Anticancer activity of Cyanothece sp. strain extracts from Egypt: First record |
topic_facet |
Anticancer Cell line Cyanothece Hydrophilic extract Molecular Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To assess the anticancer activity of eight cyanobacterial hydrophilic extracts on Ehrlich ascites carcinoma cell line. Methods: The cyanobacterial strains used in the investigation were collected from diverse habitats in Egypt. The initial cytotoxicity test of cyanobacterial hydrophilic extracts was carried out by MTT assay. The in vitro anticancer activity of the four most active extracts was performed on MCF-7 cells using sulforhodamine B assay. Morphological and molecular techniques were used to characterise identity of the isolate from which the most potent cytotoxic extract was obtained. Results: Extracts from four cyanobacterial strains had higher cytotoxic activities scoring 76.68%, 77.70%, 76.70% and 74.45%, respectively. A considerable anticancer effect was only detected when the concentrated extracts were used. One cyanobacterial extract gave the highest anticancer activity on human breast adenocarcinoma cell line (57.6% of inhibition) as compared to control. The isolate was best-matched to Cyanothece sp. with sequence resemblance 98% to Cyanothece sp. strain PCC7564 and the phylogenetic analysis confirmed its close identity to the Cyanothece genus. Conclusions: This is the first study to report the anticancer effect of aqueous extracts derived from the unicellular Cyanothece sp. from Egypt and its potential as a plausible candidate for future mass biotechnological applications. |
format |
Article in Journal/Newspaper |
author |
Nermin Adel El Semary Manar Fouda |
author_facet |
Nermin Adel El Semary Manar Fouda |
author_sort |
Nermin Adel El Semary |
title |
Anticancer activity of Cyanothece sp. strain extracts from Egypt: First record |
title_short |
Anticancer activity of Cyanothece sp. strain extracts from Egypt: First record |
title_full |
Anticancer activity of Cyanothece sp. strain extracts from Egypt: First record |
title_fullStr |
Anticancer activity of Cyanothece sp. strain extracts from Egypt: First record |
title_full_unstemmed |
Anticancer activity of Cyanothece sp. strain extracts from Egypt: First record |
title_sort |
anticancer activity of cyanothece sp. strain extracts from egypt: first record |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2015 |
url |
https://doi.org/10.1016/j.apjtb.2015.09.004 https://doaj.org/article/b37f3f0139284d61981bb4f970c5b042 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 5, Iss 12, Pp 992-995 (2015) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115002166 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2015.09.004 https://doaj.org/article/b37f3f0139284d61981bb4f970c5b042 |
op_doi |
https://doi.org/10.1016/j.apjtb.2015.09.004 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
5 |
container_issue |
12 |
container_start_page |
992 |
op_container_end_page |
995 |
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1766338682978041856 |