Optimisation of Various Physicochemical Variables Affecting Molybdenum Bioremediation Using Antarctic Bacterium, Arthrobacter sp. Strain AQ5-05
The versatility of a rare metal, molybdenum (Mo) in many industrial applications is one of the reasons why Mo is currently one of the growing environmental pollutants worldwide. Traces of inorganic contaminants, including Mo, have been discovered in Antarctica and are compromising the ecosystem. Bio...
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MDPI AG
2021
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ftdoajarticles:oai:doaj.org/article:b367797f4ada4564a65a3c563e865254 2023-05-15T13:44:03+02:00 Optimisation of Various Physicochemical Variables Affecting Molybdenum Bioremediation Using Antarctic Bacterium, Arthrobacter sp. Strain AQ5-05 Syazani Darham Sharifah Nabilah Syed-Muhaimin Kavilasni Subramaniam Azham Zulkharnain Noor Azmi Shaharuddin Khalilah Abdul Khalil Siti Aqlima Ahmad 2021-08-01T00:00:00Z https://doi.org/10.3390/w13172367 https://doaj.org/article/b367797f4ada4564a65a3c563e865254 EN eng MDPI AG https://www.mdpi.com/2073-4441/13/17/2367 https://doaj.org/toc/2073-4441 doi:10.3390/w13172367 2073-4441 https://doaj.org/article/b367797f4ada4564a65a3c563e865254 Water, Vol 13, Iss 2367, p 2367 (2021) Antarctica molybdenum microbial remediation one-factor-at-a-time (OFAT) response surface methodology (RSM) Hydraulic engineering TC1-978 Water supply for domestic and industrial purposes TD201-500 article 2021 ftdoajarticles https://doi.org/10.3390/w13172367 2022-12-31T07:19:47Z The versatility of a rare metal, molybdenum (Mo) in many industrial applications is one of the reasons why Mo is currently one of the growing environmental pollutants worldwide. Traces of inorganic contaminants, including Mo, have been discovered in Antarctica and are compromising the ecosystem. Bioremediation utilising bacteria to transform pollutants into a less toxic form is one of the approaches for solving Mo pollution. Mo reduction is a process of transforming sodium molybdate with an oxidation state of 6+ to Mo-blue, an inert version of the compound. Although there are a few Mo-reducing microbes that have been identified worldwide, only two studies were reported on the microbial reduction of Mo in Antarctica. Therefore, this study was done to assess the ability of Antarctic bacterium, Arthrobacter sp. strain AQ5-05, in reducing Mo. Optimisation of Mo reduction in Mo-supplemented media was carried out using one-factor-at-a-time (OFAT) and response surface methodology (RSM) approaches. Through OFAT, Mo was reduced optimally with substrate concentration of sucrose, ammonium sulphate, and molybdate at 1 g/L, 0.2 g/L, and 10 mM, respectively. The pH and salinity of the media were the best at 7.0 and 0.5 g/L, respectively, while the optimal temperature was at 10 °C. Further optimisation using RSM showed greater Mo-blue production in comparison to OFAT. The strain was able to stand high concentration of Mo and low temperature conditions, thus showing its potential in reducing Mo in Antarctica by employing conditions optimised by RSM. Article in Journal/Newspaper Antarc* Antarctic Antarctica Directory of Open Access Journals: DOAJ Articles Antarctic Water 13 17 2367 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Antarctica molybdenum microbial remediation one-factor-at-a-time (OFAT) response surface methodology (RSM) Hydraulic engineering TC1-978 Water supply for domestic and industrial purposes TD201-500 |
spellingShingle |
Antarctica molybdenum microbial remediation one-factor-at-a-time (OFAT) response surface methodology (RSM) Hydraulic engineering TC1-978 Water supply for domestic and industrial purposes TD201-500 Syazani Darham Sharifah Nabilah Syed-Muhaimin Kavilasni Subramaniam Azham Zulkharnain Noor Azmi Shaharuddin Khalilah Abdul Khalil Siti Aqlima Ahmad Optimisation of Various Physicochemical Variables Affecting Molybdenum Bioremediation Using Antarctic Bacterium, Arthrobacter sp. Strain AQ5-05 |
topic_facet |
Antarctica molybdenum microbial remediation one-factor-at-a-time (OFAT) response surface methodology (RSM) Hydraulic engineering TC1-978 Water supply for domestic and industrial purposes TD201-500 |
description |
The versatility of a rare metal, molybdenum (Mo) in many industrial applications is one of the reasons why Mo is currently one of the growing environmental pollutants worldwide. Traces of inorganic contaminants, including Mo, have been discovered in Antarctica and are compromising the ecosystem. Bioremediation utilising bacteria to transform pollutants into a less toxic form is one of the approaches for solving Mo pollution. Mo reduction is a process of transforming sodium molybdate with an oxidation state of 6+ to Mo-blue, an inert version of the compound. Although there are a few Mo-reducing microbes that have been identified worldwide, only two studies were reported on the microbial reduction of Mo in Antarctica. Therefore, this study was done to assess the ability of Antarctic bacterium, Arthrobacter sp. strain AQ5-05, in reducing Mo. Optimisation of Mo reduction in Mo-supplemented media was carried out using one-factor-at-a-time (OFAT) and response surface methodology (RSM) approaches. Through OFAT, Mo was reduced optimally with substrate concentration of sucrose, ammonium sulphate, and molybdate at 1 g/L, 0.2 g/L, and 10 mM, respectively. The pH and salinity of the media were the best at 7.0 and 0.5 g/L, respectively, while the optimal temperature was at 10 °C. Further optimisation using RSM showed greater Mo-blue production in comparison to OFAT. The strain was able to stand high concentration of Mo and low temperature conditions, thus showing its potential in reducing Mo in Antarctica by employing conditions optimised by RSM. |
format |
Article in Journal/Newspaper |
author |
Syazani Darham Sharifah Nabilah Syed-Muhaimin Kavilasni Subramaniam Azham Zulkharnain Noor Azmi Shaharuddin Khalilah Abdul Khalil Siti Aqlima Ahmad |
author_facet |
Syazani Darham Sharifah Nabilah Syed-Muhaimin Kavilasni Subramaniam Azham Zulkharnain Noor Azmi Shaharuddin Khalilah Abdul Khalil Siti Aqlima Ahmad |
author_sort |
Syazani Darham |
title |
Optimisation of Various Physicochemical Variables Affecting Molybdenum Bioremediation Using Antarctic Bacterium, Arthrobacter sp. Strain AQ5-05 |
title_short |
Optimisation of Various Physicochemical Variables Affecting Molybdenum Bioremediation Using Antarctic Bacterium, Arthrobacter sp. Strain AQ5-05 |
title_full |
Optimisation of Various Physicochemical Variables Affecting Molybdenum Bioremediation Using Antarctic Bacterium, Arthrobacter sp. Strain AQ5-05 |
title_fullStr |
Optimisation of Various Physicochemical Variables Affecting Molybdenum Bioremediation Using Antarctic Bacterium, Arthrobacter sp. Strain AQ5-05 |
title_full_unstemmed |
Optimisation of Various Physicochemical Variables Affecting Molybdenum Bioremediation Using Antarctic Bacterium, Arthrobacter sp. Strain AQ5-05 |
title_sort |
optimisation of various physicochemical variables affecting molybdenum bioremediation using antarctic bacterium, arthrobacter sp. strain aq5-05 |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/w13172367 https://doaj.org/article/b367797f4ada4564a65a3c563e865254 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Water, Vol 13, Iss 2367, p 2367 (2021) |
op_relation |
https://www.mdpi.com/2073-4441/13/17/2367 https://doaj.org/toc/2073-4441 doi:10.3390/w13172367 2073-4441 https://doaj.org/article/b367797f4ada4564a65a3c563e865254 |
op_doi |
https://doi.org/10.3390/w13172367 |
container_title |
Water |
container_volume |
13 |
container_issue |
17 |
container_start_page |
2367 |
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1766196422580895744 |