Short report: The potential of PCR on skin flakes from bed linens for diagnosis of scabies in an outbreak.
Background Scabies outbreaks are common in nursing homes in the Netherlands. In October 2018, a local public health service (PHS) in The Hague was notified of a new scabies outbreak in a nursing home in that region. The PHS initiated an outbreak investigation. Cases were defined as: possible (report...
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ftdoajarticles:oai:doaj.org/article:b31ac3a1b7c94f9e88f0559b88fc0d45 2023-05-15T15:09:49+02:00 Short report: The potential of PCR on skin flakes from bed linens for diagnosis of scabies in an outbreak. Raïssa Tjon-Kon-Fat Laurène Peckeu Susan Hahné Eric de Coster Wendy Tas Bas Wintermans Anneke Bergmans Mariska Petrignani Ewout Fanoy 2021-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009485 https://doaj.org/article/b31ac3a1b7c94f9e88f0559b88fc0d45 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009485 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009485 https://doaj.org/article/b31ac3a1b7c94f9e88f0559b88fc0d45 PLoS Neglected Tropical Diseases, Vol 15, Iss 6, p e0009485 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009485 2022-12-31T09:18:30Z Background Scabies outbreaks are common in nursing homes in the Netherlands. In October 2018, a local public health service (PHS) in The Hague was notified of a new scabies outbreak in a nursing home in that region. The PHS initiated an outbreak investigation. Cases were defined as: possible (reported symptoms), probable (scabies-like lesions) and confirmed (PCR or microscopy in skin flakes). Head-to-toe examinations were performed of all residents and those staff members who reported symptoms suggestive of scabies. Skin scrapings of lesions were tested either with microscopy or by PCR. Experimentally for case finding, skin flakes from bed linens of residents who reported symptoms of itchiness but did not have primary lesions were sent for PCR testing. Principal findings All residents (41) and 37/44 staff were included in this outbreak investigation. We identified 30 possible, four probable and six confirmed cases. The overall attack rate for probable/confirmed cases was 10/78 (13%). Of the six confirmed cases, two were confirmed by PCR, three by microscopy, and one showed positive findings with both techniques. Two out of the three bed-linen specimens were PCR-positive. Conclusions In this outbreak of scabies in a nursing home, PCR was used on skin flakes from bed linens, which led to the detection of two additional cases. This illustrates the potential of PCR during the investigation of scabies outbreaks. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 6 e0009485 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Raïssa Tjon-Kon-Fat Laurène Peckeu Susan Hahné Eric de Coster Wendy Tas Bas Wintermans Anneke Bergmans Mariska Petrignani Ewout Fanoy Short report: The potential of PCR on skin flakes from bed linens for diagnosis of scabies in an outbreak. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Scabies outbreaks are common in nursing homes in the Netherlands. In October 2018, a local public health service (PHS) in The Hague was notified of a new scabies outbreak in a nursing home in that region. The PHS initiated an outbreak investigation. Cases were defined as: possible (reported symptoms), probable (scabies-like lesions) and confirmed (PCR or microscopy in skin flakes). Head-to-toe examinations were performed of all residents and those staff members who reported symptoms suggestive of scabies. Skin scrapings of lesions were tested either with microscopy or by PCR. Experimentally for case finding, skin flakes from bed linens of residents who reported symptoms of itchiness but did not have primary lesions were sent for PCR testing. Principal findings All residents (41) and 37/44 staff were included in this outbreak investigation. We identified 30 possible, four probable and six confirmed cases. The overall attack rate for probable/confirmed cases was 10/78 (13%). Of the six confirmed cases, two were confirmed by PCR, three by microscopy, and one showed positive findings with both techniques. Two out of the three bed-linen specimens were PCR-positive. Conclusions In this outbreak of scabies in a nursing home, PCR was used on skin flakes from bed linens, which led to the detection of two additional cases. This illustrates the potential of PCR during the investigation of scabies outbreaks. |
format |
Article in Journal/Newspaper |
author |
Raïssa Tjon-Kon-Fat Laurène Peckeu Susan Hahné Eric de Coster Wendy Tas Bas Wintermans Anneke Bergmans Mariska Petrignani Ewout Fanoy |
author_facet |
Raïssa Tjon-Kon-Fat Laurène Peckeu Susan Hahné Eric de Coster Wendy Tas Bas Wintermans Anneke Bergmans Mariska Petrignani Ewout Fanoy |
author_sort |
Raïssa Tjon-Kon-Fat |
title |
Short report: The potential of PCR on skin flakes from bed linens for diagnosis of scabies in an outbreak. |
title_short |
Short report: The potential of PCR on skin flakes from bed linens for diagnosis of scabies in an outbreak. |
title_full |
Short report: The potential of PCR on skin flakes from bed linens for diagnosis of scabies in an outbreak. |
title_fullStr |
Short report: The potential of PCR on skin flakes from bed linens for diagnosis of scabies in an outbreak. |
title_full_unstemmed |
Short report: The potential of PCR on skin flakes from bed linens for diagnosis of scabies in an outbreak. |
title_sort |
short report: the potential of pcr on skin flakes from bed linens for diagnosis of scabies in an outbreak. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0009485 https://doaj.org/article/b31ac3a1b7c94f9e88f0559b88fc0d45 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 6, p e0009485 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0009485 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009485 https://doaj.org/article/b31ac3a1b7c94f9e88f0559b88fc0d45 |
op_doi |
https://doi.org/10.1371/journal.pntd.0009485 |
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PLOS Neglected Tropical Diseases |
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15 |
container_issue |
6 |
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e0009485 |
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