Community-based surveys for Plasmodium falciparum pfhrp2 and pfhrp3 gene deletions in selected regions of mainland Tanzania
Abstract Background Histidine-rich protein 2 (HRP2)-based malaria rapid diagnostic tests (RDTs) are effective and widely used for the detection of wild-type Plasmodium falciparum infections. Although recent studies have reported false negative HRP2 RDT results due to pfhrp2 and pfhrp3 gene deletions...
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ftdoajarticles:oai:doaj.org/article:b241218598884c6eb3dfabea46450b5a 2023-05-15T15:18:12+02:00 Community-based surveys for Plasmodium falciparum pfhrp2 and pfhrp3 gene deletions in selected regions of mainland Tanzania Catherine Bakari Sophie Jones Gireesh Subramaniam Celine I. Mandara Mercy G. Chiduo Susan Rumisha Frank Chacky Fabrizio Molteni Renata Mandike Sigsbert Mkude Ritha Njau Camelia Herman Douglas P. Nace Ally Mohamed Venkatachalam Udhayakumar Caleb K. Kibet Steven G. Nyanjom Eric Rogier Deus S. Ishengoma 2020-11-01T00:00:00Z https://doi.org/10.1186/s12936-020-03459-3 https://doaj.org/article/b241218598884c6eb3dfabea46450b5a EN eng BMC http://link.springer.com/article/10.1186/s12936-020-03459-3 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03459-3 1475-2875 https://doaj.org/article/b241218598884c6eb3dfabea46450b5a Malaria Journal, Vol 19, Iss 1, Pp 1-12 (2020) Tanzania Malaria Rapid diagnostic tests Histidine-rich protein 2/3 Lactate dehydrogenase Aldolase Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2020 ftdoajarticles https://doi.org/10.1186/s12936-020-03459-3 2022-12-30T23:31:35Z Abstract Background Histidine-rich protein 2 (HRP2)-based malaria rapid diagnostic tests (RDTs) are effective and widely used for the detection of wild-type Plasmodium falciparum infections. Although recent studies have reported false negative HRP2 RDT results due to pfhrp2 and pfhrp3 gene deletions in different countries, there is a paucity of data on the deletions of these genes in Tanzania. Methods A community-based cross-sectional survey was conducted between July and November 2017 in four regions: Geita, Kigoma, Mtwara and Ruvuma. All participants had microscopy and RDT performed in the field and provided a blood sample for laboratory multiplex antigen detection (for Plasmodium lactate dehydrogenase, aldolase, and P. falciparum HRP2). Samples showing RDT false negativity or aberrant relationship of HRP2 to pan-Plasmodium antigens were genotyped to detect the presence/absence of pfhrp2/3 genes. Results Of all samples screened by the multiplex antigen assay (n = 7543), 2417 (32.0%) were positive for any Plasmodium antigens while 5126 (68.0%) were negative for all antigens. The vast majority of the antigen positive samples contained HRP2 (2411, 99.8%), but 6 (0.2%) had only pLDH and/or aldolase without HRP2. Overall, 13 samples had an atypical relationship between a pan-Plasmodium antigen and HRP2, but were positive by PCR. An additional 16 samples with negative HRP2 RDT results but P. falciparum positive by microscopy were also chosen for pfhrp2/3 genotyping. The summation of false negative RDT results and laboratory antigen results provided 35 total samples with confirmed P. falciparum DNA for pfhrp2/3 genotyping. Of the 35 samples, 4 (11.4%) failed to consistently amplify positive control genes; pfmsp1 and pfmsp2 and were excluded from the analysis. The pfhrp2 and pfhrp3 genes were successfully amplified in the remaining 31 (88.6%) samples, confirming an absence of deletions in these genes. Conclusions This study provides evidence that P. falciparum parasites in the study area have no deletions of both ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Geita ENVELOPE(6.006,6.006,62.555,62.555) Malaria Journal 19 1 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Tanzania Malaria Rapid diagnostic tests Histidine-rich protein 2/3 Lactate dehydrogenase Aldolase Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Tanzania Malaria Rapid diagnostic tests Histidine-rich protein 2/3 Lactate dehydrogenase Aldolase Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Catherine Bakari Sophie Jones Gireesh Subramaniam Celine I. Mandara Mercy G. Chiduo Susan Rumisha Frank Chacky Fabrizio Molteni Renata Mandike Sigsbert Mkude Ritha Njau Camelia Herman Douglas P. Nace Ally Mohamed Venkatachalam Udhayakumar Caleb K. Kibet Steven G. Nyanjom Eric Rogier Deus S. Ishengoma Community-based surveys for Plasmodium falciparum pfhrp2 and pfhrp3 gene deletions in selected regions of mainland Tanzania |
topic_facet |
Tanzania Malaria Rapid diagnostic tests Histidine-rich protein 2/3 Lactate dehydrogenase Aldolase Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Histidine-rich protein 2 (HRP2)-based malaria rapid diagnostic tests (RDTs) are effective and widely used for the detection of wild-type Plasmodium falciparum infections. Although recent studies have reported false negative HRP2 RDT results due to pfhrp2 and pfhrp3 gene deletions in different countries, there is a paucity of data on the deletions of these genes in Tanzania. Methods A community-based cross-sectional survey was conducted between July and November 2017 in four regions: Geita, Kigoma, Mtwara and Ruvuma. All participants had microscopy and RDT performed in the field and provided a blood sample for laboratory multiplex antigen detection (for Plasmodium lactate dehydrogenase, aldolase, and P. falciparum HRP2). Samples showing RDT false negativity or aberrant relationship of HRP2 to pan-Plasmodium antigens were genotyped to detect the presence/absence of pfhrp2/3 genes. Results Of all samples screened by the multiplex antigen assay (n = 7543), 2417 (32.0%) were positive for any Plasmodium antigens while 5126 (68.0%) were negative for all antigens. The vast majority of the antigen positive samples contained HRP2 (2411, 99.8%), but 6 (0.2%) had only pLDH and/or aldolase without HRP2. Overall, 13 samples had an atypical relationship between a pan-Plasmodium antigen and HRP2, but were positive by PCR. An additional 16 samples with negative HRP2 RDT results but P. falciparum positive by microscopy were also chosen for pfhrp2/3 genotyping. The summation of false negative RDT results and laboratory antigen results provided 35 total samples with confirmed P. falciparum DNA for pfhrp2/3 genotyping. Of the 35 samples, 4 (11.4%) failed to consistently amplify positive control genes; pfmsp1 and pfmsp2 and were excluded from the analysis. The pfhrp2 and pfhrp3 genes were successfully amplified in the remaining 31 (88.6%) samples, confirming an absence of deletions in these genes. Conclusions This study provides evidence that P. falciparum parasites in the study area have no deletions of both ... |
format |
Article in Journal/Newspaper |
author |
Catherine Bakari Sophie Jones Gireesh Subramaniam Celine I. Mandara Mercy G. Chiduo Susan Rumisha Frank Chacky Fabrizio Molteni Renata Mandike Sigsbert Mkude Ritha Njau Camelia Herman Douglas P. Nace Ally Mohamed Venkatachalam Udhayakumar Caleb K. Kibet Steven G. Nyanjom Eric Rogier Deus S. Ishengoma |
author_facet |
Catherine Bakari Sophie Jones Gireesh Subramaniam Celine I. Mandara Mercy G. Chiduo Susan Rumisha Frank Chacky Fabrizio Molteni Renata Mandike Sigsbert Mkude Ritha Njau Camelia Herman Douglas P. Nace Ally Mohamed Venkatachalam Udhayakumar Caleb K. Kibet Steven G. Nyanjom Eric Rogier Deus S. Ishengoma |
author_sort |
Catherine Bakari |
title |
Community-based surveys for Plasmodium falciparum pfhrp2 and pfhrp3 gene deletions in selected regions of mainland Tanzania |
title_short |
Community-based surveys for Plasmodium falciparum pfhrp2 and pfhrp3 gene deletions in selected regions of mainland Tanzania |
title_full |
Community-based surveys for Plasmodium falciparum pfhrp2 and pfhrp3 gene deletions in selected regions of mainland Tanzania |
title_fullStr |
Community-based surveys for Plasmodium falciparum pfhrp2 and pfhrp3 gene deletions in selected regions of mainland Tanzania |
title_full_unstemmed |
Community-based surveys for Plasmodium falciparum pfhrp2 and pfhrp3 gene deletions in selected regions of mainland Tanzania |
title_sort |
community-based surveys for plasmodium falciparum pfhrp2 and pfhrp3 gene deletions in selected regions of mainland tanzania |
publisher |
BMC |
publishDate |
2020 |
url |
https://doi.org/10.1186/s12936-020-03459-3 https://doaj.org/article/b241218598884c6eb3dfabea46450b5a |
long_lat |
ENVELOPE(6.006,6.006,62.555,62.555) |
geographic |
Arctic Geita |
geographic_facet |
Arctic Geita |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 19, Iss 1, Pp 1-12 (2020) |
op_relation |
http://link.springer.com/article/10.1186/s12936-020-03459-3 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03459-3 1475-2875 https://doaj.org/article/b241218598884c6eb3dfabea46450b5a |
op_doi |
https://doi.org/10.1186/s12936-020-03459-3 |
container_title |
Malaria Journal |
container_volume |
19 |
container_issue |
1 |
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1766348421073993728 |