Enveloped and non-enveloped viral-like particles in Trypanosoma cruzi epimastigotes
ABSTRACT Electron microscopy is routinely used to identify viral infections in protozoan parasites. These viruses have been described as non-enveloped and icosahedral structures with a diameter of 30-60 nm. Most of them are classified within the non-segmented dsRNA Totiviridae family. We observed vi...
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ftdoajarticles:oai:doaj.org/article:b232a75da66b41a9833d4af8c3908834 2024-09-09T19:26:26+00:00 Enveloped and non-enveloped viral-like particles in Trypanosoma cruzi epimastigotes Ana María Fernández-Presas Luis Padilla-Noriega Ingeborg-Becker Lilia Robert José Agustín Jiménez Sandra Solano Jose Delgado Patricia Tato José Luis Molinari https://doi.org/10.1590/s1678-9946201759046 https://doaj.org/article/b232a75da66b41a9833d4af8c3908834 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652017005000228&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946201759046 https://doaj.org/article/b232a75da66b41a9833d4af8c3908834 Revista do Instituto de Medicina Tropical de São Paulo, Vol 59, Iss 0 Trypanosoma cruzi Arthropod-borne viruses Protozoan infections Flaviviridae Totiviridae Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article ftdoajarticles https://doi.org/10.1590/s1678-9946201759046 2024-08-05T17:49:30Z ABSTRACT Electron microscopy is routinely used to identify viral infections in protozoan parasites. These viruses have been described as non-enveloped and icosahedral structures with a diameter of 30-60 nm. Most of them are classified within the non-segmented dsRNA Totiviridae family. We observed virus-like particles (VLPs) through transmission electron microscopy in the cytoplasm of Trypanosoma cruzi epimastigotes grown in cultures. Clusters of electrodense enveloped VLPs having a diameter of 48 nm were also observed. These clusters appear to have been released from distended Golgi cisternae. Furthermore, a paracrystalline array of electrodense, non-enveloped VLPs (with a diameter of 32 nm) were found in distended Golgi cisternae or as smaller clusters at a distance from the RE or Golgi. We cannot rule out that the 48 nm enveloped VLPs belong to the ssRNA Flaviviridae family because they are within its size range. The localization of enveloped VLPs is consistent with the replication strategy of these viruses that transit through the Golgi to be released at the cell surface. Due to the size and shape of the 32 nm non-enveloped VLPs, we propose that they belong to the dsRNA Totiviridae family. This is the first description of cytoplasmic enveloped and non-enveloped VLPs in T. cruzi epimastigotes. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 59 0 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Trypanosoma cruzi Arthropod-borne viruses Protozoan infections Flaviviridae Totiviridae Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Trypanosoma cruzi Arthropod-borne viruses Protozoan infections Flaviviridae Totiviridae Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Ana María Fernández-Presas Luis Padilla-Noriega Ingeborg-Becker Lilia Robert José Agustín Jiménez Sandra Solano Jose Delgado Patricia Tato José Luis Molinari Enveloped and non-enveloped viral-like particles in Trypanosoma cruzi epimastigotes |
topic_facet |
Trypanosoma cruzi Arthropod-borne viruses Protozoan infections Flaviviridae Totiviridae Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
ABSTRACT Electron microscopy is routinely used to identify viral infections in protozoan parasites. These viruses have been described as non-enveloped and icosahedral structures with a diameter of 30-60 nm. Most of them are classified within the non-segmented dsRNA Totiviridae family. We observed virus-like particles (VLPs) through transmission electron microscopy in the cytoplasm of Trypanosoma cruzi epimastigotes grown in cultures. Clusters of electrodense enveloped VLPs having a diameter of 48 nm were also observed. These clusters appear to have been released from distended Golgi cisternae. Furthermore, a paracrystalline array of electrodense, non-enveloped VLPs (with a diameter of 32 nm) were found in distended Golgi cisternae or as smaller clusters at a distance from the RE or Golgi. We cannot rule out that the 48 nm enveloped VLPs belong to the ssRNA Flaviviridae family because they are within its size range. The localization of enveloped VLPs is consistent with the replication strategy of these viruses that transit through the Golgi to be released at the cell surface. Due to the size and shape of the 32 nm non-enveloped VLPs, we propose that they belong to the dsRNA Totiviridae family. This is the first description of cytoplasmic enveloped and non-enveloped VLPs in T. cruzi epimastigotes. |
format |
Article in Journal/Newspaper |
author |
Ana María Fernández-Presas Luis Padilla-Noriega Ingeborg-Becker Lilia Robert José Agustín Jiménez Sandra Solano Jose Delgado Patricia Tato José Luis Molinari |
author_facet |
Ana María Fernández-Presas Luis Padilla-Noriega Ingeborg-Becker Lilia Robert José Agustín Jiménez Sandra Solano Jose Delgado Patricia Tato José Luis Molinari |
author_sort |
Ana María Fernández-Presas |
title |
Enveloped and non-enveloped viral-like particles in Trypanosoma cruzi epimastigotes |
title_short |
Enveloped and non-enveloped viral-like particles in Trypanosoma cruzi epimastigotes |
title_full |
Enveloped and non-enveloped viral-like particles in Trypanosoma cruzi epimastigotes |
title_fullStr |
Enveloped and non-enveloped viral-like particles in Trypanosoma cruzi epimastigotes |
title_full_unstemmed |
Enveloped and non-enveloped viral-like particles in Trypanosoma cruzi epimastigotes |
title_sort |
enveloped and non-enveloped viral-like particles in trypanosoma cruzi epimastigotes |
publisher |
Universidade de São Paulo (USP) |
url |
https://doi.org/10.1590/s1678-9946201759046 https://doaj.org/article/b232a75da66b41a9833d4af8c3908834 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 59, Iss 0 |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652017005000228&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946201759046 https://doaj.org/article/b232a75da66b41a9833d4af8c3908834 |
op_doi |
https://doi.org/10.1590/s1678-9946201759046 |
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Revista do Instituto de Medicina Tropical de São Paulo |
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59 |
container_issue |
0 |
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1809896044944687104 |