Tailoring Hydrothermal Vent Biodiversity Toward Improved Biodiscovery Using a Novel in situ Enrichment Strategy

Deep-sea hydrothermal vents are amongst the most extreme environments on Earth and represent interesting targets for marine bioprospecting and biodiscovery. The microbial communities in hydrothermal vents are often dominated by chemolithoautotrophs utilizing simple chemical compounds, though the ful...

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Published in:Frontiers in Microbiology
Main Authors: Runar Stokke, Eoghan P. Reeves, Håkon Dahle, Anita-Elin Fedøy, Thomas Viflot, Solveig Lie Onstad, Francesca Vulcano, Rolf B. Pedersen, Vincent G. H. Eijsink, Ida H. Steen
Format: Article in Journal/Newspaper
Language:English
Published: Frontiers Media S.A. 2020
Subjects:
Online Access:https://doi.org/10.3389/fmicb.2020.00249
https://doaj.org/article/b224bb719b8e4b199ccf787b8349db07
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spelling ftdoajarticles:oai:doaj.org/article:b224bb719b8e4b199ccf787b8349db07 2023-05-15T16:57:03+02:00 Tailoring Hydrothermal Vent Biodiversity Toward Improved Biodiscovery Using a Novel in situ Enrichment Strategy Runar Stokke Eoghan P. Reeves Håkon Dahle Anita-Elin Fedøy Thomas Viflot Solveig Lie Onstad Francesca Vulcano Rolf B. Pedersen Vincent G. H. Eijsink Ida H. Steen 2020-02-01T00:00:00Z https://doi.org/10.3389/fmicb.2020.00249 https://doaj.org/article/b224bb719b8e4b199ccf787b8349db07 EN eng Frontiers Media S.A. https://www.frontiersin.org/article/10.3389/fmicb.2020.00249/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2020.00249 https://doaj.org/article/b224bb719b8e4b199ccf787b8349db07 Frontiers in Microbiology, Vol 11 (2020) deep sea hydrothermal sediments in situ enrichment marine bioprospecting biotechnology Microbiology QR1-502 article 2020 ftdoajarticles https://doi.org/10.3389/fmicb.2020.00249 2022-12-31T14:06:24Z Deep-sea hydrothermal vents are amongst the most extreme environments on Earth and represent interesting targets for marine bioprospecting and biodiscovery. The microbial communities in hydrothermal vents are often dominated by chemolithoautotrophs utilizing simple chemical compounds, though the full extent of their heterotrophic abilities is still being explored. In the bioprocessing industry, where degradation of complex organic materials often is a major challenge, new microbial solutions are heavily needed. To meet these needs, we have developed novel in situ incubators and tested if deployment of recalcitrant materials from fish farming and wood-pulping industries introduced changes in the microbial community structure in hot marine hydrothermal sediments. The incubation chambers were deployed in sediments at the Bruse vent site located within the Jan Mayen vent field for 1 year, after which the microbial populations in the chambers were profiled by 16S rRNA Ion Torrent amplicon sequencing. A total of 921 operational taxonomic units (OTUs) were assigned into 74 different phyla where differences in community structure were observed depending on the incubated material, chamber depth below the sea floor and/or temperature. A high fraction of putative heterotrophic microbial lineages related to cultivated members within the Thermotogales were observed. However, considerable fractions of previously uncultivated and novel Thermotogales and Bacteroidetes were also identified. Moreover, several novel lineages (e.g., members within the DPANN superphylum, unidentified archaeal lineages, unclassified Thermoplasmatales and Candidatus division BRC-1 bacterium) of as-yet uncultivated thermophilic archaea and bacteria were identified. Overall, our data illustrate that amendment of hydrothermal vent communities by in situ incubation of biomass induces shifts in community structure toward increased fractions of heterotrophic microorganisms. The technologies utilized here could aid in subsequent metagenomics-based enzyme ... Article in Journal/Newspaper Jan Mayen Directory of Open Access Journals: DOAJ Articles Jan Mayen Frontiers in Microbiology 11
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic deep sea
hydrothermal sediments
in situ enrichment
marine bioprospecting
biotechnology
Microbiology
QR1-502
spellingShingle deep sea
hydrothermal sediments
in situ enrichment
marine bioprospecting
biotechnology
Microbiology
QR1-502
Runar Stokke
Eoghan P. Reeves
Håkon Dahle
Anita-Elin Fedøy
Thomas Viflot
Solveig Lie Onstad
Francesca Vulcano
Rolf B. Pedersen
Vincent G. H. Eijsink
Ida H. Steen
Tailoring Hydrothermal Vent Biodiversity Toward Improved Biodiscovery Using a Novel in situ Enrichment Strategy
topic_facet deep sea
hydrothermal sediments
in situ enrichment
marine bioprospecting
biotechnology
Microbiology
QR1-502
description Deep-sea hydrothermal vents are amongst the most extreme environments on Earth and represent interesting targets for marine bioprospecting and biodiscovery. The microbial communities in hydrothermal vents are often dominated by chemolithoautotrophs utilizing simple chemical compounds, though the full extent of their heterotrophic abilities is still being explored. In the bioprocessing industry, where degradation of complex organic materials often is a major challenge, new microbial solutions are heavily needed. To meet these needs, we have developed novel in situ incubators and tested if deployment of recalcitrant materials from fish farming and wood-pulping industries introduced changes in the microbial community structure in hot marine hydrothermal sediments. The incubation chambers were deployed in sediments at the Bruse vent site located within the Jan Mayen vent field for 1 year, after which the microbial populations in the chambers were profiled by 16S rRNA Ion Torrent amplicon sequencing. A total of 921 operational taxonomic units (OTUs) were assigned into 74 different phyla where differences in community structure were observed depending on the incubated material, chamber depth below the sea floor and/or temperature. A high fraction of putative heterotrophic microbial lineages related to cultivated members within the Thermotogales were observed. However, considerable fractions of previously uncultivated and novel Thermotogales and Bacteroidetes were also identified. Moreover, several novel lineages (e.g., members within the DPANN superphylum, unidentified archaeal lineages, unclassified Thermoplasmatales and Candidatus division BRC-1 bacterium) of as-yet uncultivated thermophilic archaea and bacteria were identified. Overall, our data illustrate that amendment of hydrothermal vent communities by in situ incubation of biomass induces shifts in community structure toward increased fractions of heterotrophic microorganisms. The technologies utilized here could aid in subsequent metagenomics-based enzyme ...
format Article in Journal/Newspaper
author Runar Stokke
Eoghan P. Reeves
Håkon Dahle
Anita-Elin Fedøy
Thomas Viflot
Solveig Lie Onstad
Francesca Vulcano
Rolf B. Pedersen
Vincent G. H. Eijsink
Ida H. Steen
author_facet Runar Stokke
Eoghan P. Reeves
Håkon Dahle
Anita-Elin Fedøy
Thomas Viflot
Solveig Lie Onstad
Francesca Vulcano
Rolf B. Pedersen
Vincent G. H. Eijsink
Ida H. Steen
author_sort Runar Stokke
title Tailoring Hydrothermal Vent Biodiversity Toward Improved Biodiscovery Using a Novel in situ Enrichment Strategy
title_short Tailoring Hydrothermal Vent Biodiversity Toward Improved Biodiscovery Using a Novel in situ Enrichment Strategy
title_full Tailoring Hydrothermal Vent Biodiversity Toward Improved Biodiscovery Using a Novel in situ Enrichment Strategy
title_fullStr Tailoring Hydrothermal Vent Biodiversity Toward Improved Biodiscovery Using a Novel in situ Enrichment Strategy
title_full_unstemmed Tailoring Hydrothermal Vent Biodiversity Toward Improved Biodiscovery Using a Novel in situ Enrichment Strategy
title_sort tailoring hydrothermal vent biodiversity toward improved biodiscovery using a novel in situ enrichment strategy
publisher Frontiers Media S.A.
publishDate 2020
url https://doi.org/10.3389/fmicb.2020.00249
https://doaj.org/article/b224bb719b8e4b199ccf787b8349db07
geographic Jan Mayen
geographic_facet Jan Mayen
genre Jan Mayen
genre_facet Jan Mayen
op_source Frontiers in Microbiology, Vol 11 (2020)
op_relation https://www.frontiersin.org/article/10.3389/fmicb.2020.00249/full
https://doaj.org/toc/1664-302X
1664-302X
doi:10.3389/fmicb.2020.00249
https://doaj.org/article/b224bb719b8e4b199ccf787b8349db07
op_doi https://doi.org/10.3389/fmicb.2020.00249
container_title Frontiers in Microbiology
container_volume 11
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