Tailoring Hydrothermal Vent Biodiversity Toward Improved Biodiscovery Using a Novel in situ Enrichment Strategy
Deep-sea hydrothermal vents are amongst the most extreme environments on Earth and represent interesting targets for marine bioprospecting and biodiscovery. The microbial communities in hydrothermal vents are often dominated by chemolithoautotrophs utilizing simple chemical compounds, though the ful...
Published in: | Frontiers in Microbiology |
---|---|
Main Authors: | , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Frontiers Media S.A.
2020
|
Subjects: | |
Online Access: | https://doi.org/10.3389/fmicb.2020.00249 https://doaj.org/article/b224bb719b8e4b199ccf787b8349db07 |
id |
ftdoajarticles:oai:doaj.org/article:b224bb719b8e4b199ccf787b8349db07 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:b224bb719b8e4b199ccf787b8349db07 2023-05-15T16:57:03+02:00 Tailoring Hydrothermal Vent Biodiversity Toward Improved Biodiscovery Using a Novel in situ Enrichment Strategy Runar Stokke Eoghan P. Reeves Håkon Dahle Anita-Elin Fedøy Thomas Viflot Solveig Lie Onstad Francesca Vulcano Rolf B. Pedersen Vincent G. H. Eijsink Ida H. Steen 2020-02-01T00:00:00Z https://doi.org/10.3389/fmicb.2020.00249 https://doaj.org/article/b224bb719b8e4b199ccf787b8349db07 EN eng Frontiers Media S.A. https://www.frontiersin.org/article/10.3389/fmicb.2020.00249/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2020.00249 https://doaj.org/article/b224bb719b8e4b199ccf787b8349db07 Frontiers in Microbiology, Vol 11 (2020) deep sea hydrothermal sediments in situ enrichment marine bioprospecting biotechnology Microbiology QR1-502 article 2020 ftdoajarticles https://doi.org/10.3389/fmicb.2020.00249 2022-12-31T14:06:24Z Deep-sea hydrothermal vents are amongst the most extreme environments on Earth and represent interesting targets for marine bioprospecting and biodiscovery. The microbial communities in hydrothermal vents are often dominated by chemolithoautotrophs utilizing simple chemical compounds, though the full extent of their heterotrophic abilities is still being explored. In the bioprocessing industry, where degradation of complex organic materials often is a major challenge, new microbial solutions are heavily needed. To meet these needs, we have developed novel in situ incubators and tested if deployment of recalcitrant materials from fish farming and wood-pulping industries introduced changes in the microbial community structure in hot marine hydrothermal sediments. The incubation chambers were deployed in sediments at the Bruse vent site located within the Jan Mayen vent field for 1 year, after which the microbial populations in the chambers were profiled by 16S rRNA Ion Torrent amplicon sequencing. A total of 921 operational taxonomic units (OTUs) were assigned into 74 different phyla where differences in community structure were observed depending on the incubated material, chamber depth below the sea floor and/or temperature. A high fraction of putative heterotrophic microbial lineages related to cultivated members within the Thermotogales were observed. However, considerable fractions of previously uncultivated and novel Thermotogales and Bacteroidetes were also identified. Moreover, several novel lineages (e.g., members within the DPANN superphylum, unidentified archaeal lineages, unclassified Thermoplasmatales and Candidatus division BRC-1 bacterium) of as-yet uncultivated thermophilic archaea and bacteria were identified. Overall, our data illustrate that amendment of hydrothermal vent communities by in situ incubation of biomass induces shifts in community structure toward increased fractions of heterotrophic microorganisms. The technologies utilized here could aid in subsequent metagenomics-based enzyme ... Article in Journal/Newspaper Jan Mayen Directory of Open Access Journals: DOAJ Articles Jan Mayen Frontiers in Microbiology 11 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
deep sea hydrothermal sediments in situ enrichment marine bioprospecting biotechnology Microbiology QR1-502 |
spellingShingle |
deep sea hydrothermal sediments in situ enrichment marine bioprospecting biotechnology Microbiology QR1-502 Runar Stokke Eoghan P. Reeves Håkon Dahle Anita-Elin Fedøy Thomas Viflot Solveig Lie Onstad Francesca Vulcano Rolf B. Pedersen Vincent G. H. Eijsink Ida H. Steen Tailoring Hydrothermal Vent Biodiversity Toward Improved Biodiscovery Using a Novel in situ Enrichment Strategy |
topic_facet |
deep sea hydrothermal sediments in situ enrichment marine bioprospecting biotechnology Microbiology QR1-502 |
description |
Deep-sea hydrothermal vents are amongst the most extreme environments on Earth and represent interesting targets for marine bioprospecting and biodiscovery. The microbial communities in hydrothermal vents are often dominated by chemolithoautotrophs utilizing simple chemical compounds, though the full extent of their heterotrophic abilities is still being explored. In the bioprocessing industry, where degradation of complex organic materials often is a major challenge, new microbial solutions are heavily needed. To meet these needs, we have developed novel in situ incubators and tested if deployment of recalcitrant materials from fish farming and wood-pulping industries introduced changes in the microbial community structure in hot marine hydrothermal sediments. The incubation chambers were deployed in sediments at the Bruse vent site located within the Jan Mayen vent field for 1 year, after which the microbial populations in the chambers were profiled by 16S rRNA Ion Torrent amplicon sequencing. A total of 921 operational taxonomic units (OTUs) were assigned into 74 different phyla where differences in community structure were observed depending on the incubated material, chamber depth below the sea floor and/or temperature. A high fraction of putative heterotrophic microbial lineages related to cultivated members within the Thermotogales were observed. However, considerable fractions of previously uncultivated and novel Thermotogales and Bacteroidetes were also identified. Moreover, several novel lineages (e.g., members within the DPANN superphylum, unidentified archaeal lineages, unclassified Thermoplasmatales and Candidatus division BRC-1 bacterium) of as-yet uncultivated thermophilic archaea and bacteria were identified. Overall, our data illustrate that amendment of hydrothermal vent communities by in situ incubation of biomass induces shifts in community structure toward increased fractions of heterotrophic microorganisms. The technologies utilized here could aid in subsequent metagenomics-based enzyme ... |
format |
Article in Journal/Newspaper |
author |
Runar Stokke Eoghan P. Reeves Håkon Dahle Anita-Elin Fedøy Thomas Viflot Solveig Lie Onstad Francesca Vulcano Rolf B. Pedersen Vincent G. H. Eijsink Ida H. Steen |
author_facet |
Runar Stokke Eoghan P. Reeves Håkon Dahle Anita-Elin Fedøy Thomas Viflot Solveig Lie Onstad Francesca Vulcano Rolf B. Pedersen Vincent G. H. Eijsink Ida H. Steen |
author_sort |
Runar Stokke |
title |
Tailoring Hydrothermal Vent Biodiversity Toward Improved Biodiscovery Using a Novel in situ Enrichment Strategy |
title_short |
Tailoring Hydrothermal Vent Biodiversity Toward Improved Biodiscovery Using a Novel in situ Enrichment Strategy |
title_full |
Tailoring Hydrothermal Vent Biodiversity Toward Improved Biodiscovery Using a Novel in situ Enrichment Strategy |
title_fullStr |
Tailoring Hydrothermal Vent Biodiversity Toward Improved Biodiscovery Using a Novel in situ Enrichment Strategy |
title_full_unstemmed |
Tailoring Hydrothermal Vent Biodiversity Toward Improved Biodiscovery Using a Novel in situ Enrichment Strategy |
title_sort |
tailoring hydrothermal vent biodiversity toward improved biodiscovery using a novel in situ enrichment strategy |
publisher |
Frontiers Media S.A. |
publishDate |
2020 |
url |
https://doi.org/10.3389/fmicb.2020.00249 https://doaj.org/article/b224bb719b8e4b199ccf787b8349db07 |
geographic |
Jan Mayen |
geographic_facet |
Jan Mayen |
genre |
Jan Mayen |
genre_facet |
Jan Mayen |
op_source |
Frontiers in Microbiology, Vol 11 (2020) |
op_relation |
https://www.frontiersin.org/article/10.3389/fmicb.2020.00249/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2020.00249 https://doaj.org/article/b224bb719b8e4b199ccf787b8349db07 |
op_doi |
https://doi.org/10.3389/fmicb.2020.00249 |
container_title |
Frontiers in Microbiology |
container_volume |
11 |
_version_ |
1766048319441731584 |