Characterization of five microsatellites in the Siberian sturgeon Acipenser baerii from aquaculture
Sturgeons, a class of fish with a particular scientific and commercial importance have been overexploited through overfishing and poaching, nowadays being at the brink of extinction. The developments of the molecular techniques allow the detection of intraspecific genetic differences and could ensur...
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ftdoajarticles:oai:doaj.org/article:b20673caa2284a74860f27b4d4f2ac9f 2023-05-15T13:01:40+02:00 Characterization of five microsatellites in the Siberian sturgeon Acipenser baerii from aquaculture Sergiu Emil Georgescu 2013-05-01T00:00:00Z https://doaj.org/article/b20673caa2284a74860f27b4d4f2ac9f EN eng Agroprint Timisoara http://spasb.ro/index.php/spasb/article/view/40 https://doaj.org/toc/1841-9364 https://doaj.org/toc/2344-4576 1841-9364 2344-4576 https://doaj.org/article/b20673caa2284a74860f27b4d4f2ac9f Scientific Papers Animal Science and Biotechnologies, Vol 46, Iss 1, Pp 95-98 (2013) siberian sturgeon microsatellites aquaculture molecular tool Agriculture S Technology T Science Q article 2013 ftdoajarticles 2022-12-31T02:13:11Z Sturgeons, a class of fish with a particular scientific and commercial importance have been overexploited through overfishing and poaching, nowadays being at the brink of extinction. The developments of the molecular techniques allow the detection of intraspecific genetic differences and could ensure an increased ability to characterize the extent of genetic variation in sturgeon populations, both aquaculture and wildlife. In our study we optimized a protocol for microsatellites amplification in Siberian sturgeon, Acipenser baerii. The microsatellite markers were originally designed for the American lake sturgeon, Acipenser fulvescens, Adriatic sturgeon, Acipenser naccari and Atlantic sturgeon, Acipenser oxyrinchus oxyrinchus. We successfully amplified five microsatellite loci (LS 19, LS 68, Aox 9, Aox 45, AnacE4) obtaining allele peaks of different sizes which were analyzed by capilary electrophoresis. Except the locus AnacE4 that presented a low polymorphism, the other four loci were displaying minimum 6 alleles for each locus. Therefore, caracterization of these new markers increase the panel of molecular tools available for fish farmers to monitoring levels of genetic variation within stocks. Article in Journal/Newspaper Acipenser baerii Siberian sturgeon Directory of Open Access Journals: DOAJ Articles American Lake ENVELOPE(-106.701,-106.701,57.200,57.200) |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
siberian sturgeon microsatellites aquaculture molecular tool Agriculture S Technology T Science Q |
spellingShingle |
siberian sturgeon microsatellites aquaculture molecular tool Agriculture S Technology T Science Q Sergiu Emil Georgescu Characterization of five microsatellites in the Siberian sturgeon Acipenser baerii from aquaculture |
topic_facet |
siberian sturgeon microsatellites aquaculture molecular tool Agriculture S Technology T Science Q |
description |
Sturgeons, a class of fish with a particular scientific and commercial importance have been overexploited through overfishing and poaching, nowadays being at the brink of extinction. The developments of the molecular techniques allow the detection of intraspecific genetic differences and could ensure an increased ability to characterize the extent of genetic variation in sturgeon populations, both aquaculture and wildlife. In our study we optimized a protocol for microsatellites amplification in Siberian sturgeon, Acipenser baerii. The microsatellite markers were originally designed for the American lake sturgeon, Acipenser fulvescens, Adriatic sturgeon, Acipenser naccari and Atlantic sturgeon, Acipenser oxyrinchus oxyrinchus. We successfully amplified five microsatellite loci (LS 19, LS 68, Aox 9, Aox 45, AnacE4) obtaining allele peaks of different sizes which were analyzed by capilary electrophoresis. Except the locus AnacE4 that presented a low polymorphism, the other four loci were displaying minimum 6 alleles for each locus. Therefore, caracterization of these new markers increase the panel of molecular tools available for fish farmers to monitoring levels of genetic variation within stocks. |
format |
Article in Journal/Newspaper |
author |
Sergiu Emil Georgescu |
author_facet |
Sergiu Emil Georgescu |
author_sort |
Sergiu Emil Georgescu |
title |
Characterization of five microsatellites in the Siberian sturgeon Acipenser baerii from aquaculture |
title_short |
Characterization of five microsatellites in the Siberian sturgeon Acipenser baerii from aquaculture |
title_full |
Characterization of five microsatellites in the Siberian sturgeon Acipenser baerii from aquaculture |
title_fullStr |
Characterization of five microsatellites in the Siberian sturgeon Acipenser baerii from aquaculture |
title_full_unstemmed |
Characterization of five microsatellites in the Siberian sturgeon Acipenser baerii from aquaculture |
title_sort |
characterization of five microsatellites in the siberian sturgeon acipenser baerii from aquaculture |
publisher |
Agroprint Timisoara |
publishDate |
2013 |
url |
https://doaj.org/article/b20673caa2284a74860f27b4d4f2ac9f |
long_lat |
ENVELOPE(-106.701,-106.701,57.200,57.200) |
geographic |
American Lake |
geographic_facet |
American Lake |
genre |
Acipenser baerii Siberian sturgeon |
genre_facet |
Acipenser baerii Siberian sturgeon |
op_source |
Scientific Papers Animal Science and Biotechnologies, Vol 46, Iss 1, Pp 95-98 (2013) |
op_relation |
http://spasb.ro/index.php/spasb/article/view/40 https://doaj.org/toc/1841-9364 https://doaj.org/toc/2344-4576 1841-9364 2344-4576 https://doaj.org/article/b20673caa2284a74860f27b4d4f2ac9f |
_version_ |
1766283505848811520 |