A secreted Heat shock protein 90 of Trichomonas vaginalis.
Trichomonas vaginalis is a causative agent of Trichomoniasis, a leading non-viral sexually transmitted disease worldwide. In the current study, we show Heat shock protein 90 is essential for its growth. Upon genomic analysis of the parasite, it was found to possess seven ORFs which could potentially...
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ftdoajarticles:oai:doaj.org/article:b203b19651814ca2a42b3baacba37fdc 2023-05-15T15:05:08+02:00 A secreted Heat shock protein 90 of Trichomonas vaginalis. Meetali Singh Divya Beri Rishi Kumar Nageshan Leena Chavaan Darshak Gadara Mukta Poojary Suraj Subramaniam Utpal Tatu 2018-05-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006493 https://doaj.org/article/b203b19651814ca2a42b3baacba37fdc EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5973626?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006493 https://doaj.org/article/b203b19651814ca2a42b3baacba37fdc PLoS Neglected Tropical Diseases, Vol 12, Iss 5, p e0006493 (2018) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2018 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006493 2022-12-31T09:35:55Z Trichomonas vaginalis is a causative agent of Trichomoniasis, a leading non-viral sexually transmitted disease worldwide. In the current study, we show Heat shock protein 90 is essential for its growth. Upon genomic analysis of the parasite, it was found to possess seven ORFs which could potentially encode Hsp90 isoforms. We identified a cytosolic Hsp90 homolog, four homologs which can align to truncated cytosolic Hsp90 gene products along with two Grp94 homologs (ER isoform of Hsp90). However, both Grp94 orthologs lacked an ER retention motif. In cancer cells, it is very well established that Hsp90 is secreted and regulates key clients involved in metastases, migration, and invasion. Since Trichomonas Grp94 lacks ER retention motif, we examined the possibility of its secretion. By using cell biology and biochemical approaches we show that the Grp94 isoform of Hsp90 is secreted by the parasite by the classical ER-Golgi pathway. This is the first report of a genome encoded secreted Hsp90 in a clinically important parasitic protozoan. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 12 5 e0006493 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Meetali Singh Divya Beri Rishi Kumar Nageshan Leena Chavaan Darshak Gadara Mukta Poojary Suraj Subramaniam Utpal Tatu A secreted Heat shock protein 90 of Trichomonas vaginalis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Trichomonas vaginalis is a causative agent of Trichomoniasis, a leading non-viral sexually transmitted disease worldwide. In the current study, we show Heat shock protein 90 is essential for its growth. Upon genomic analysis of the parasite, it was found to possess seven ORFs which could potentially encode Hsp90 isoforms. We identified a cytosolic Hsp90 homolog, four homologs which can align to truncated cytosolic Hsp90 gene products along with two Grp94 homologs (ER isoform of Hsp90). However, both Grp94 orthologs lacked an ER retention motif. In cancer cells, it is very well established that Hsp90 is secreted and regulates key clients involved in metastases, migration, and invasion. Since Trichomonas Grp94 lacks ER retention motif, we examined the possibility of its secretion. By using cell biology and biochemical approaches we show that the Grp94 isoform of Hsp90 is secreted by the parasite by the classical ER-Golgi pathway. This is the first report of a genome encoded secreted Hsp90 in a clinically important parasitic protozoan. |
format |
Article in Journal/Newspaper |
author |
Meetali Singh Divya Beri Rishi Kumar Nageshan Leena Chavaan Darshak Gadara Mukta Poojary Suraj Subramaniam Utpal Tatu |
author_facet |
Meetali Singh Divya Beri Rishi Kumar Nageshan Leena Chavaan Darshak Gadara Mukta Poojary Suraj Subramaniam Utpal Tatu |
author_sort |
Meetali Singh |
title |
A secreted Heat shock protein 90 of Trichomonas vaginalis. |
title_short |
A secreted Heat shock protein 90 of Trichomonas vaginalis. |
title_full |
A secreted Heat shock protein 90 of Trichomonas vaginalis. |
title_fullStr |
A secreted Heat shock protein 90 of Trichomonas vaginalis. |
title_full_unstemmed |
A secreted Heat shock protein 90 of Trichomonas vaginalis. |
title_sort |
secreted heat shock protein 90 of trichomonas vaginalis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
https://doi.org/10.1371/journal.pntd.0006493 https://doaj.org/article/b203b19651814ca2a42b3baacba37fdc |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 12, Iss 5, p e0006493 (2018) |
op_relation |
http://europepmc.org/articles/PMC5973626?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006493 https://doaj.org/article/b203b19651814ca2a42b3baacba37fdc |
op_doi |
https://doi.org/10.1371/journal.pntd.0006493 |
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PLOS Neglected Tropical Diseases |
container_volume |
12 |
container_issue |
5 |
container_start_page |
e0006493 |
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1766336887599923200 |