The existence of only one haplotype of Leishmania major in the main and potential reservoir hosts of zoonotic cutaneous leishmaniasis using different molecular markers in a focal area in Iran
Introduction Leishmania major is the causative agent of zoonotic cutaneous leishmaniasis (ZCL), and great gerbils are the main reservoir hosts in Iran. Abarkouh in central Iran is an emerging focal point for which the reservoir hosts of ZCL are unclear. This research project was designed to detect a...
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Online Access: | https://doi.org/10.1590/0037-8682-0164-2014 https://doaj.org/article/b1df6d37073746b7b4804a716ae7a738 |
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ftdoajarticles:oai:doaj.org/article:b1df6d37073746b7b4804a716ae7a738 2023-05-15T15:12:49+02:00 The existence of only one haplotype of Leishmania major in the main and potential reservoir hosts of zoonotic cutaneous leishmaniasis using different molecular markers in a focal area in Iran Narmin Najafzadeh Mohammad Mehdi Sedaghat Syed Shuja Sultan Adel Spotin Alireza Zamani Roozbeh Taslimian Amir Yaghoubinezhad Parviz Parvizi 2014-10-01T00:00:00Z https://doi.org/10.1590/0037-8682-0164-2014 https://doaj.org/article/b1df6d37073746b7b4804a716ae7a738 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822014000500599&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0164-2014 https://doaj.org/article/b1df6d37073746b7b4804a716ae7a738 Revista da Sociedade Brasileira de Medicina Tropical, Vol 47, Iss 5, Pp 599-606 (2014) Leishmania parasite Zoonotic cutaneous leishmaniasis Rodents Haplotype Iran Arctic medicine. Tropical medicine RC955-962 article 2014 ftdoajarticles https://doi.org/10.1590/0037-8682-0164-2014 2022-12-31T02:47:33Z Introduction Leishmania major is the causative agent of zoonotic cutaneous leishmaniasis (ZCL), and great gerbils are the main reservoir hosts in Iran. Abarkouh in central Iran is an emerging focal point for which the reservoir hosts of ZCL are unclear. This research project was designed to detect any Leishmania parasites in different wild rodent species. Methods All rodents captured in 2011 and 2012 from Abarkouh district were identified based on morphological characteristics and by amplification of the rodent cytochrome b (Cyt b) gene. To detect Leishmania infection in rodents, deoxyribonucleic acid (DNA) of each ear was extracted. Internal transcribed spacer-ribosomal deoxyribonucleic acid (ITS-rDNA), microsatellites, kinetoplast deoxyribonucleic acid (kDNA) and cytochrome b genes of Leishmania parasites were amplified by polymerase chain reaction (PCR). Restriction fragment length polymorphism (RFLP) and sequencing were employed to confirm the Leishmania identification. Results Of 68 captured rodents in the region, 55 Rhombomys opimus were identified and nine Leishmania infections (9/55) were found. In addition, eight Meriones libycus and two Tatera indica were sampled, and one of each was confirmed to be infected. Two Meriones persicus and one Mus musculus were sampled with no infection. Conclusions The results showed that all 11 unambiguously positive Leishmania infections were Leishmania major. Only one haplotype of L. major (GenBank access No. EF413075) was found and at least three rodents R. opimus, M. libycus and T. indica—appear to be the main and potential reservoir hosts in this ZCL focus. The reservoir hosts are variable and versatile in small ZCL focal locations. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 47 5 599 606 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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Leishmania parasite Zoonotic cutaneous leishmaniasis Rodents Haplotype Iran Arctic medicine. Tropical medicine RC955-962 |
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Leishmania parasite Zoonotic cutaneous leishmaniasis Rodents Haplotype Iran Arctic medicine. Tropical medicine RC955-962 Narmin Najafzadeh Mohammad Mehdi Sedaghat Syed Shuja Sultan Adel Spotin Alireza Zamani Roozbeh Taslimian Amir Yaghoubinezhad Parviz Parvizi The existence of only one haplotype of Leishmania major in the main and potential reservoir hosts of zoonotic cutaneous leishmaniasis using different molecular markers in a focal area in Iran |
topic_facet |
Leishmania parasite Zoonotic cutaneous leishmaniasis Rodents Haplotype Iran Arctic medicine. Tropical medicine RC955-962 |
description |
Introduction Leishmania major is the causative agent of zoonotic cutaneous leishmaniasis (ZCL), and great gerbils are the main reservoir hosts in Iran. Abarkouh in central Iran is an emerging focal point for which the reservoir hosts of ZCL are unclear. This research project was designed to detect any Leishmania parasites in different wild rodent species. Methods All rodents captured in 2011 and 2012 from Abarkouh district were identified based on morphological characteristics and by amplification of the rodent cytochrome b (Cyt b) gene. To detect Leishmania infection in rodents, deoxyribonucleic acid (DNA) of each ear was extracted. Internal transcribed spacer-ribosomal deoxyribonucleic acid (ITS-rDNA), microsatellites, kinetoplast deoxyribonucleic acid (kDNA) and cytochrome b genes of Leishmania parasites were amplified by polymerase chain reaction (PCR). Restriction fragment length polymorphism (RFLP) and sequencing were employed to confirm the Leishmania identification. Results Of 68 captured rodents in the region, 55 Rhombomys opimus were identified and nine Leishmania infections (9/55) were found. In addition, eight Meriones libycus and two Tatera indica were sampled, and one of each was confirmed to be infected. Two Meriones persicus and one Mus musculus were sampled with no infection. Conclusions The results showed that all 11 unambiguously positive Leishmania infections were Leishmania major. Only one haplotype of L. major (GenBank access No. EF413075) was found and at least three rodents R. opimus, M. libycus and T. indica—appear to be the main and potential reservoir hosts in this ZCL focus. The reservoir hosts are variable and versatile in small ZCL focal locations. |
format |
Article in Journal/Newspaper |
author |
Narmin Najafzadeh Mohammad Mehdi Sedaghat Syed Shuja Sultan Adel Spotin Alireza Zamani Roozbeh Taslimian Amir Yaghoubinezhad Parviz Parvizi |
author_facet |
Narmin Najafzadeh Mohammad Mehdi Sedaghat Syed Shuja Sultan Adel Spotin Alireza Zamani Roozbeh Taslimian Amir Yaghoubinezhad Parviz Parvizi |
author_sort |
Narmin Najafzadeh |
title |
The existence of only one haplotype of Leishmania major in the main and potential reservoir hosts of zoonotic cutaneous leishmaniasis using different molecular markers in a focal area in Iran |
title_short |
The existence of only one haplotype of Leishmania major in the main and potential reservoir hosts of zoonotic cutaneous leishmaniasis using different molecular markers in a focal area in Iran |
title_full |
The existence of only one haplotype of Leishmania major in the main and potential reservoir hosts of zoonotic cutaneous leishmaniasis using different molecular markers in a focal area in Iran |
title_fullStr |
The existence of only one haplotype of Leishmania major in the main and potential reservoir hosts of zoonotic cutaneous leishmaniasis using different molecular markers in a focal area in Iran |
title_full_unstemmed |
The existence of only one haplotype of Leishmania major in the main and potential reservoir hosts of zoonotic cutaneous leishmaniasis using different molecular markers in a focal area in Iran |
title_sort |
existence of only one haplotype of leishmania major in the main and potential reservoir hosts of zoonotic cutaneous leishmaniasis using different molecular markers in a focal area in iran |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2014 |
url |
https://doi.org/10.1590/0037-8682-0164-2014 https://doaj.org/article/b1df6d37073746b7b4804a716ae7a738 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 47, Iss 5, Pp 599-606 (2014) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822014000500599&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0164-2014 https://doaj.org/article/b1df6d37073746b7b4804a716ae7a738 |
op_doi |
https://doi.org/10.1590/0037-8682-0164-2014 |
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Revista da Sociedade Brasileira de Medicina Tropical |
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47 |
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599 |
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