Atlantic salmon reovirus infection causes a CD8 T cell myocarditis in Atlantic salmon (Salmo salar L.).
Heart and skeletal inflammation (HSMI) of farmed Atlantic salmon (Salmo salar L.) is a disease characterized by a chronic myocarditis involving the epicardium and the compact and spongious part of the heart ventricle. Chronic myositis of the red skeletal muscle is also a typical finding of HSMI. Pis...
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ftdoajarticles:oai:doaj.org/article:b1d936b7f939458aa6b42364fd0073bc 2023-05-15T15:28:16+02:00 Atlantic salmon reovirus infection causes a CD8 T cell myocarditis in Atlantic salmon (Salmo salar L.). Aase B Mikalsen Oyvind Haugland Marit Rode Inge Tom Solbakk Oystein Evensen 2012-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0037269 https://doaj.org/article/b1d936b7f939458aa6b42364fd0073bc EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3367920?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0037269 https://doaj.org/article/b1d936b7f939458aa6b42364fd0073bc PLoS ONE, Vol 7, Iss 6, p e37269 (2012) Medicine R Science Q article 2012 ftdoajarticles https://doi.org/10.1371/journal.pone.0037269 2022-12-31T11:56:40Z Heart and skeletal inflammation (HSMI) of farmed Atlantic salmon (Salmo salar L.) is a disease characterized by a chronic myocarditis involving the epicardium and the compact and spongious part of the heart ventricle. Chronic myositis of the red skeletal muscle is also a typical finding of HSMI. Piscine reovirus (PRV) has been detected by real-time PCR from farmed and wild salmon with and without typical changes of HSMI and thus the causal relationship between presence of virus and the disease has not been fully determined. In this study we show that the Atlantic salmon reovirus (ASRV), identical to PRV, can be passaged in GF-1 cells and experimental challenge of naïve Atlantic salmon with cell culture passaged reovirus results in cardiac and skeletal muscle pathology typical of HSMI with onset of pathology from 6 weeks, peaking by 9 weeks post challenge. ASRV replicates in heart tissue and the peak level of virus replication coincides with peak of heart lesions. We further demonstrate mRNA transcript assessment and in situ characterization that challenged fish develop a CD8+ T cell myocarditis. Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles PLoS ONE 7 6 e37269 |
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Directory of Open Access Journals: DOAJ Articles |
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Medicine R Science Q |
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Medicine R Science Q Aase B Mikalsen Oyvind Haugland Marit Rode Inge Tom Solbakk Oystein Evensen Atlantic salmon reovirus infection causes a CD8 T cell myocarditis in Atlantic salmon (Salmo salar L.). |
topic_facet |
Medicine R Science Q |
description |
Heart and skeletal inflammation (HSMI) of farmed Atlantic salmon (Salmo salar L.) is a disease characterized by a chronic myocarditis involving the epicardium and the compact and spongious part of the heart ventricle. Chronic myositis of the red skeletal muscle is also a typical finding of HSMI. Piscine reovirus (PRV) has been detected by real-time PCR from farmed and wild salmon with and without typical changes of HSMI and thus the causal relationship between presence of virus and the disease has not been fully determined. In this study we show that the Atlantic salmon reovirus (ASRV), identical to PRV, can be passaged in GF-1 cells and experimental challenge of naïve Atlantic salmon with cell culture passaged reovirus results in cardiac and skeletal muscle pathology typical of HSMI with onset of pathology from 6 weeks, peaking by 9 weeks post challenge. ASRV replicates in heart tissue and the peak level of virus replication coincides with peak of heart lesions. We further demonstrate mRNA transcript assessment and in situ characterization that challenged fish develop a CD8+ T cell myocarditis. |
format |
Article in Journal/Newspaper |
author |
Aase B Mikalsen Oyvind Haugland Marit Rode Inge Tom Solbakk Oystein Evensen |
author_facet |
Aase B Mikalsen Oyvind Haugland Marit Rode Inge Tom Solbakk Oystein Evensen |
author_sort |
Aase B Mikalsen |
title |
Atlantic salmon reovirus infection causes a CD8 T cell myocarditis in Atlantic salmon (Salmo salar L.). |
title_short |
Atlantic salmon reovirus infection causes a CD8 T cell myocarditis in Atlantic salmon (Salmo salar L.). |
title_full |
Atlantic salmon reovirus infection causes a CD8 T cell myocarditis in Atlantic salmon (Salmo salar L.). |
title_fullStr |
Atlantic salmon reovirus infection causes a CD8 T cell myocarditis in Atlantic salmon (Salmo salar L.). |
title_full_unstemmed |
Atlantic salmon reovirus infection causes a CD8 T cell myocarditis in Atlantic salmon (Salmo salar L.). |
title_sort |
atlantic salmon reovirus infection causes a cd8 t cell myocarditis in atlantic salmon (salmo salar l.). |
publisher |
Public Library of Science (PLoS) |
publishDate |
2012 |
url |
https://doi.org/10.1371/journal.pone.0037269 https://doaj.org/article/b1d936b7f939458aa6b42364fd0073bc |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
PLoS ONE, Vol 7, Iss 6, p e37269 (2012) |
op_relation |
http://europepmc.org/articles/PMC3367920?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0037269 https://doaj.org/article/b1d936b7f939458aa6b42364fd0073bc |
op_doi |
https://doi.org/10.1371/journal.pone.0037269 |
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PLoS ONE |
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7 |
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6 |
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e37269 |
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1766358642599133184 |