Comparative Genomic Analysis of Agarolytic Flavobacterium faecale WV33 T
Flavobacteria are widely dispersed in a variety of environments and produce various polysaccharide-degrading enzymes. Here, we report the complete genome of Flavobacterium faecale WV33 T , an agar-degrading bacterium isolated from the stools of Antarctic penguins. The sequenced genome of F. faecale...
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ftdoajarticles:oai:doaj.org/article:b1c2837cc779408381d78331cca163d8 2023-05-15T13:32:37+02:00 Comparative Genomic Analysis of Agarolytic Flavobacterium faecale WV33 T Jun Ho Lee Seong-Rae Lee Sejong Han Pyung Cheon Lee 2022-09-01T00:00:00Z https://doi.org/10.3390/ijms231810884 https://doaj.org/article/b1c2837cc779408381d78331cca163d8 EN eng MDPI AG https://www.mdpi.com/1422-0067/23/18/10884 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms231810884 1422-0067 1661-6596 https://doaj.org/article/b1c2837cc779408381d78331cca163d8 International Journal of Molecular Sciences, Vol 23, Iss 10884, p 10884 (2022) agarase CAZymes Flavobacterium faecale comparative genomics Biology (General) QH301-705.5 Chemistry QD1-999 article 2022 ftdoajarticles https://doi.org/10.3390/ijms231810884 2022-12-31T00:33:48Z Flavobacteria are widely dispersed in a variety of environments and produce various polysaccharide-degrading enzymes. Here, we report the complete genome of Flavobacterium faecale WV33 T , an agar-degrading bacterium isolated from the stools of Antarctic penguins. The sequenced genome of F. faecale WV33 T represents a single circular chromosome (4,621,116 bp, 35.2% G + C content), containing 3984 coding DNA sequences and 85 RNA-coding genes. The genome of F. faecale WV33 T contains 154 genes that encode carbohydrate-active enzymes (CAZymes). Among the CAZymes, seven putative genes encoding agarases have been identified in the genome. Transcriptional analysis revealed that the expression of these putative agarases was significantly enhanced by the presence of agar in the culture medium, suggesting that these proteins are involved in agar hydrolysis. Pangenome analysis revealed that the genomes of the 27 Flavobacterium type strains, including F. faecale WV33 T , tend to be very plastic, and Flavobacterium strains are unique species with a tiny core genome and a large non-core region. The average nucleotide identity and phylogenomic analysis of the 27 Flavobacterium -type strains showed that F. faecale WV33 T was positioned in a unique clade in the evolutionary tree. Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic International Journal of Molecular Sciences 23 18 10884 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
agarase CAZymes Flavobacterium faecale comparative genomics Biology (General) QH301-705.5 Chemistry QD1-999 |
spellingShingle |
agarase CAZymes Flavobacterium faecale comparative genomics Biology (General) QH301-705.5 Chemistry QD1-999 Jun Ho Lee Seong-Rae Lee Sejong Han Pyung Cheon Lee Comparative Genomic Analysis of Agarolytic Flavobacterium faecale WV33 T |
topic_facet |
agarase CAZymes Flavobacterium faecale comparative genomics Biology (General) QH301-705.5 Chemistry QD1-999 |
description |
Flavobacteria are widely dispersed in a variety of environments and produce various polysaccharide-degrading enzymes. Here, we report the complete genome of Flavobacterium faecale WV33 T , an agar-degrading bacterium isolated from the stools of Antarctic penguins. The sequenced genome of F. faecale WV33 T represents a single circular chromosome (4,621,116 bp, 35.2% G + C content), containing 3984 coding DNA sequences and 85 RNA-coding genes. The genome of F. faecale WV33 T contains 154 genes that encode carbohydrate-active enzymes (CAZymes). Among the CAZymes, seven putative genes encoding agarases have been identified in the genome. Transcriptional analysis revealed that the expression of these putative agarases was significantly enhanced by the presence of agar in the culture medium, suggesting that these proteins are involved in agar hydrolysis. Pangenome analysis revealed that the genomes of the 27 Flavobacterium type strains, including F. faecale WV33 T , tend to be very plastic, and Flavobacterium strains are unique species with a tiny core genome and a large non-core region. The average nucleotide identity and phylogenomic analysis of the 27 Flavobacterium -type strains showed that F. faecale WV33 T was positioned in a unique clade in the evolutionary tree. |
format |
Article in Journal/Newspaper |
author |
Jun Ho Lee Seong-Rae Lee Sejong Han Pyung Cheon Lee |
author_facet |
Jun Ho Lee Seong-Rae Lee Sejong Han Pyung Cheon Lee |
author_sort |
Jun Ho Lee |
title |
Comparative Genomic Analysis of Agarolytic Flavobacterium faecale WV33 T |
title_short |
Comparative Genomic Analysis of Agarolytic Flavobacterium faecale WV33 T |
title_full |
Comparative Genomic Analysis of Agarolytic Flavobacterium faecale WV33 T |
title_fullStr |
Comparative Genomic Analysis of Agarolytic Flavobacterium faecale WV33 T |
title_full_unstemmed |
Comparative Genomic Analysis of Agarolytic Flavobacterium faecale WV33 T |
title_sort |
comparative genomic analysis of agarolytic flavobacterium faecale wv33 t |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/ijms231810884 https://doaj.org/article/b1c2837cc779408381d78331cca163d8 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
International Journal of Molecular Sciences, Vol 23, Iss 10884, p 10884 (2022) |
op_relation |
https://www.mdpi.com/1422-0067/23/18/10884 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms231810884 1422-0067 1661-6596 https://doaj.org/article/b1c2837cc779408381d78331cca163d8 |
op_doi |
https://doi.org/10.3390/ijms231810884 |
container_title |
International Journal of Molecular Sciences |
container_volume |
23 |
container_issue |
18 |
container_start_page |
10884 |
_version_ |
1766028693021392896 |