Gut bacteria influence Blastocystis sp. phenotypes and may trigger pathogenicity.
Whilst the influence of intestinal microbiota has been shown in many diseases such as irritable bowel syndrome, colorectal cancer, and aging, investigations are still scarce on its role in altering the nature of other infective organisms. Here we studied the association and interaction of Blastocyst...
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ftdoajarticles:oai:doaj.org/article:b15b430f728c4563939171061f828c9a 2023-06-06T11:51:26+02:00 Gut bacteria influence Blastocystis sp. phenotypes and may trigger pathogenicity. Arutchelvan Rajamanikam Mohd Noor Mat Isa Chandramathi Samudi Sridevi Devaraj Suresh Kumar Govind 2023-03-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0011170 https://doaj.org/article/b15b430f728c4563939171061f828c9a EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0011170 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011170 https://doaj.org/article/b15b430f728c4563939171061f828c9a PLoS Neglected Tropical Diseases, Vol 17, Iss 3, p e0011170 (2023) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2023 ftdoajarticles https://doi.org/10.1371/journal.pntd.0011170 2023-04-16T00:39:21Z Whilst the influence of intestinal microbiota has been shown in many diseases such as irritable bowel syndrome, colorectal cancer, and aging, investigations are still scarce on its role in altering the nature of other infective organisms. Here we studied the association and interaction of Blastocystis sp. and human intestinal microbiota. In this study, we investigated the gut microbiome of Blastocystis sp.-free and Blastocystis sp. ST3-infected individuals who are symptomatic and asymptomatic. We tested if the expression of phenotype and pathogenic characteristics of Blastocystis sp. ST3 was influenced by the alteration of its accompanying microbiota. Blastocystis sp. ST3 infection alters bacterial composition. Its presence in asymptomatic individuals showed a significant effect on microbial richness compared to symptomatic ones. Inferred metagenomic findings suggest that colonization of Blastocystis sp. ST3 could contribute to the alteration of microbial functions. For the first time, we demonstrate the influence of bacteria on Blastocystis sp. pathogenicity. When Blastocystis sp. isolated from a symptomatic individual was co-cultured with bacterial suspension of Blastocystis sp. from an asymptomatic individual, the parasite demonstrated increased growth and reduced potential pathogenic expressions. This study also reveals that Blastocystis sp. infection could influence microbial functions without much effect on the microbiota diversity itself. Our results also demonstrate evidence on the influential role of gut microbiota in altering the characteristics of the parasite, which becomes the basis for the contradictory findings on the parasite's pathogenic role seen across different studies. Our study provides evidence that asymptomatic Blastocystis sp. in a human gut can be triggered to show pathogenic characteristics when influenced by the intestinal microbiota. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 17 3 e0011170 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Arutchelvan Rajamanikam Mohd Noor Mat Isa Chandramathi Samudi Sridevi Devaraj Suresh Kumar Govind Gut bacteria influence Blastocystis sp. phenotypes and may trigger pathogenicity. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Whilst the influence of intestinal microbiota has been shown in many diseases such as irritable bowel syndrome, colorectal cancer, and aging, investigations are still scarce on its role in altering the nature of other infective organisms. Here we studied the association and interaction of Blastocystis sp. and human intestinal microbiota. In this study, we investigated the gut microbiome of Blastocystis sp.-free and Blastocystis sp. ST3-infected individuals who are symptomatic and asymptomatic. We tested if the expression of phenotype and pathogenic characteristics of Blastocystis sp. ST3 was influenced by the alteration of its accompanying microbiota. Blastocystis sp. ST3 infection alters bacterial composition. Its presence in asymptomatic individuals showed a significant effect on microbial richness compared to symptomatic ones. Inferred metagenomic findings suggest that colonization of Blastocystis sp. ST3 could contribute to the alteration of microbial functions. For the first time, we demonstrate the influence of bacteria on Blastocystis sp. pathogenicity. When Blastocystis sp. isolated from a symptomatic individual was co-cultured with bacterial suspension of Blastocystis sp. from an asymptomatic individual, the parasite demonstrated increased growth and reduced potential pathogenic expressions. This study also reveals that Blastocystis sp. infection could influence microbial functions without much effect on the microbiota diversity itself. Our results also demonstrate evidence on the influential role of gut microbiota in altering the characteristics of the parasite, which becomes the basis for the contradictory findings on the parasite's pathogenic role seen across different studies. Our study provides evidence that asymptomatic Blastocystis sp. in a human gut can be triggered to show pathogenic characteristics when influenced by the intestinal microbiota. |
format |
Article in Journal/Newspaper |
author |
Arutchelvan Rajamanikam Mohd Noor Mat Isa Chandramathi Samudi Sridevi Devaraj Suresh Kumar Govind |
author_facet |
Arutchelvan Rajamanikam Mohd Noor Mat Isa Chandramathi Samudi Sridevi Devaraj Suresh Kumar Govind |
author_sort |
Arutchelvan Rajamanikam |
title |
Gut bacteria influence Blastocystis sp. phenotypes and may trigger pathogenicity. |
title_short |
Gut bacteria influence Blastocystis sp. phenotypes and may trigger pathogenicity. |
title_full |
Gut bacteria influence Blastocystis sp. phenotypes and may trigger pathogenicity. |
title_fullStr |
Gut bacteria influence Blastocystis sp. phenotypes and may trigger pathogenicity. |
title_full_unstemmed |
Gut bacteria influence Blastocystis sp. phenotypes and may trigger pathogenicity. |
title_sort |
gut bacteria influence blastocystis sp. phenotypes and may trigger pathogenicity. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2023 |
url |
https://doi.org/10.1371/journal.pntd.0011170 https://doaj.org/article/b15b430f728c4563939171061f828c9a |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 17, Iss 3, p e0011170 (2023) |
op_relation |
https://doi.org/10.1371/journal.pntd.0011170 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011170 https://doaj.org/article/b15b430f728c4563939171061f828c9a |
op_doi |
https://doi.org/10.1371/journal.pntd.0011170 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
17 |
container_issue |
3 |
container_start_page |
e0011170 |
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1767957136249192448 |