Expression Analysis of Moritella viscosa -Challenged Atlantic Salmon Identifies Disease-Responding Genes, MicroRNAs and Their Predicted Target Genes and Pathways
Moritella viscosa is a bacterial pathogen causing winter-ulcer disease in Atlantic salmon. The lesions on affected fish lead to increased mortality, decreased fish welfare, and inferior meat quality in farmed salmon. MicroRNAs (miRNAs) are small non-coding RNAs involved in post-transcriptional regul...
Published in: | International Journal of Molecular Sciences |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
MDPI AG
2022
|
Subjects: | |
Online Access: | https://doi.org/10.3390/ijms231911200 https://doaj.org/article/b125fc7f030f4d3bb9bbe7c0835c2984 |
id |
ftdoajarticles:oai:doaj.org/article:b125fc7f030f4d3bb9bbe7c0835c2984 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:b125fc7f030f4d3bb9bbe7c0835c2984 2023-05-15T15:30:51+02:00 Expression Analysis of Moritella viscosa -Challenged Atlantic Salmon Identifies Disease-Responding Genes, MicroRNAs and Their Predicted Target Genes and Pathways Sigmund Ramberg Aleksei Krasnov Duncan Colquhoun Christian Wallace Rune Andreassen 2022-09-01T00:00:00Z https://doi.org/10.3390/ijms231911200 https://doaj.org/article/b125fc7f030f4d3bb9bbe7c0835c2984 EN eng MDPI AG https://www.mdpi.com/1422-0067/23/19/11200 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms231911200 1422-0067 1661-6596 https://doaj.org/article/b125fc7f030f4d3bb9bbe7c0835c2984 International Journal of Molecular Sciences, Vol 23, Iss 11200, p 11200 (2022) winter-ulcer disease response microRNA small-RNA sequencing Atlantic salmon head kidney Biology (General) QH301-705.5 Chemistry QD1-999 article 2022 ftdoajarticles https://doi.org/10.3390/ijms231911200 2022-12-30T20:22:20Z Moritella viscosa is a bacterial pathogen causing winter-ulcer disease in Atlantic salmon. The lesions on affected fish lead to increased mortality, decreased fish welfare, and inferior meat quality in farmed salmon. MicroRNAs (miRNAs) are small non-coding RNAs involved in post-transcriptional regulation by guiding the miRNA-induced silencing complex to specific mRNA transcripts (target genes). The goal of this study was to identify miRNAs responding to Moritella viscosa in salmon by investigating miRNA expression in the head-kidney and the muscle/skin from lesion sites caused by the pathogen. Protein coding gene expression was investigated by microarray analysis in the same materials. Seventeen differentially expressed guide-miRNAs (gDE-miRNAs) were identified in the head-kidney, and thirty-nine in lesion sites, while the microarray analysis reproduced the differential expression signature of several thousand genes known as infection-responsive. In silico target prediction and enrichment analysis suggested that the gDE-miRNAs were predicted to target genes involved in immune responses, hemostasis, angiogenesis, stress responses, metabolism, cell growth, and apoptosis. The majority of the conserved gDE-miRNAs (e.g., miR-125, miR-132, miR-146, miR-152, miR-155, miR-223 and miR-2188) are known as infection-responsive in other vertebrates. Collectively, the findings indicate that gDE-miRNAs are important post-transcriptional gene regulators of the host response to bacterial infection. Article in Journal/Newspaper Atlantic salmon Directory of Open Access Journals: DOAJ Articles International Journal of Molecular Sciences 23 19 11200 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
winter-ulcer disease response microRNA small-RNA sequencing Atlantic salmon head kidney Biology (General) QH301-705.5 Chemistry QD1-999 |
spellingShingle |
winter-ulcer disease response microRNA small-RNA sequencing Atlantic salmon head kidney Biology (General) QH301-705.5 Chemistry QD1-999 Sigmund Ramberg Aleksei Krasnov Duncan Colquhoun Christian Wallace Rune Andreassen Expression Analysis of Moritella viscosa -Challenged Atlantic Salmon Identifies Disease-Responding Genes, MicroRNAs and Their Predicted Target Genes and Pathways |
topic_facet |
winter-ulcer disease response microRNA small-RNA sequencing Atlantic salmon head kidney Biology (General) QH301-705.5 Chemistry QD1-999 |
description |
Moritella viscosa is a bacterial pathogen causing winter-ulcer disease in Atlantic salmon. The lesions on affected fish lead to increased mortality, decreased fish welfare, and inferior meat quality in farmed salmon. MicroRNAs (miRNAs) are small non-coding RNAs involved in post-transcriptional regulation by guiding the miRNA-induced silencing complex to specific mRNA transcripts (target genes). The goal of this study was to identify miRNAs responding to Moritella viscosa in salmon by investigating miRNA expression in the head-kidney and the muscle/skin from lesion sites caused by the pathogen. Protein coding gene expression was investigated by microarray analysis in the same materials. Seventeen differentially expressed guide-miRNAs (gDE-miRNAs) were identified in the head-kidney, and thirty-nine in lesion sites, while the microarray analysis reproduced the differential expression signature of several thousand genes known as infection-responsive. In silico target prediction and enrichment analysis suggested that the gDE-miRNAs were predicted to target genes involved in immune responses, hemostasis, angiogenesis, stress responses, metabolism, cell growth, and apoptosis. The majority of the conserved gDE-miRNAs (e.g., miR-125, miR-132, miR-146, miR-152, miR-155, miR-223 and miR-2188) are known as infection-responsive in other vertebrates. Collectively, the findings indicate that gDE-miRNAs are important post-transcriptional gene regulators of the host response to bacterial infection. |
format |
Article in Journal/Newspaper |
author |
Sigmund Ramberg Aleksei Krasnov Duncan Colquhoun Christian Wallace Rune Andreassen |
author_facet |
Sigmund Ramberg Aleksei Krasnov Duncan Colquhoun Christian Wallace Rune Andreassen |
author_sort |
Sigmund Ramberg |
title |
Expression Analysis of Moritella viscosa -Challenged Atlantic Salmon Identifies Disease-Responding Genes, MicroRNAs and Their Predicted Target Genes and Pathways |
title_short |
Expression Analysis of Moritella viscosa -Challenged Atlantic Salmon Identifies Disease-Responding Genes, MicroRNAs and Their Predicted Target Genes and Pathways |
title_full |
Expression Analysis of Moritella viscosa -Challenged Atlantic Salmon Identifies Disease-Responding Genes, MicroRNAs and Their Predicted Target Genes and Pathways |
title_fullStr |
Expression Analysis of Moritella viscosa -Challenged Atlantic Salmon Identifies Disease-Responding Genes, MicroRNAs and Their Predicted Target Genes and Pathways |
title_full_unstemmed |
Expression Analysis of Moritella viscosa -Challenged Atlantic Salmon Identifies Disease-Responding Genes, MicroRNAs and Their Predicted Target Genes and Pathways |
title_sort |
expression analysis of moritella viscosa -challenged atlantic salmon identifies disease-responding genes, micrornas and their predicted target genes and pathways |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/ijms231911200 https://doaj.org/article/b125fc7f030f4d3bb9bbe7c0835c2984 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
International Journal of Molecular Sciences, Vol 23, Iss 11200, p 11200 (2022) |
op_relation |
https://www.mdpi.com/1422-0067/23/19/11200 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms231911200 1422-0067 1661-6596 https://doaj.org/article/b125fc7f030f4d3bb9bbe7c0835c2984 |
op_doi |
https://doi.org/10.3390/ijms231911200 |
container_title |
International Journal of Molecular Sciences |
container_volume |
23 |
container_issue |
19 |
container_start_page |
11200 |
_version_ |
1766361320973664256 |