Radiometric detection of metabolic activity of Paracoccidioides brasiliensis and its susceptibility to amphotericin B and diethylstilbestrol
Paracoccidioidomycosis (South American blastomycosis) is a systemic disease, strikingly more frequent in males, caused by the dimorphic fungus Paracoccidioides brasiliensis. A radiometric assay system has been applied to study the metabolic activity and the effect of drugs on this fungus "in vi...
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Universidade de São Paulo (USP)
1987
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Online Access: | https://doi.org/10.1590/S0036-46651987000500005 https://doaj.org/article/b104e73659b04bf7b585355a7ac3d306 |
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ftdoajarticles:oai:doaj.org/article:b104e73659b04bf7b585355a7ac3d306 2024-09-09T19:26:48+00:00 Radiometric detection of metabolic activity of Paracoccidioides brasiliensis and its susceptibility to amphotericin B and diethylstilbestrol Edwaldo E. Camargo Maria K. Sato Gilda M. B. Del Negro Carlos da Silva Lacaz 1987-10-01T00:00:00Z https://doi.org/10.1590/S0036-46651987000500005 https://doaj.org/article/b104e73659b04bf7b585355a7ac3d306 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651987000500005&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46651987000500005 https://doaj.org/article/b104e73659b04bf7b585355a7ac3d306 Revista do Instituto de Medicina Tropical de São Paulo, Vol 29, Iss 5, Pp 289-294 (1987) Paracoccidioides brasiliensis Radiometric assay 14C-Substrates Amphotericin B Diethylstilbestrol Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 1987 ftdoajarticles https://doi.org/10.1590/S0036-46651987000500005 2024-08-05T17:49:30Z Paracoccidioidomycosis (South American blastomycosis) is a systemic disease, strikingly more frequent in males, caused by the dimorphic fungus Paracoccidioides brasiliensis. A radiometric assay system has been applied to study the metabolic activity and the effect of drugs on this fungus "in vitro". The Y form of the yeast, grown in liquid Sabouraud medium was inoculated into sterile reaction vials containing the 6B aerobic medium along with 2.0 μCi of 14C-substrates. Control vials, prepared in the same way, contained autoclaved fungi. To study the effects of amphotericin B (AB) (0.1 and 10 μg/ml) and diethylstilbestrol (DSB) (1.0, 5.0 and 10 μg/ml) extra controls with live fungi and no drug were used. All vials were incubated at 35°C and metabolism measured daily with a Bactec instrument. 14CO2 production by P. brasiliensis was slow and could be followed for as long as 50 days. AB at 10mg/ml and DSB at 5 μg/ml inhibited the metabolism and had a cidal effect on this fungus. The results with DSB might explain the low incidence of the disease in females. This technique shows promise for studying metabolic pathways, investi gating more convenient 14C-substrates to expedite radiometric detection and for monitoring the effects of other drugs and factors on the metabolism of P. brasiliensis "in vitro". Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic The ''Y'' ENVELOPE(-112.453,-112.453,57.591,57.591) Revista do Instituto de Medicina Tropical de São Paulo 29 5 289 294 |
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topic |
Paracoccidioides brasiliensis Radiometric assay 14C-Substrates Amphotericin B Diethylstilbestrol Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Paracoccidioides brasiliensis Radiometric assay 14C-Substrates Amphotericin B Diethylstilbestrol Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Edwaldo E. Camargo Maria K. Sato Gilda M. B. Del Negro Carlos da Silva Lacaz Radiometric detection of metabolic activity of Paracoccidioides brasiliensis and its susceptibility to amphotericin B and diethylstilbestrol |
topic_facet |
Paracoccidioides brasiliensis Radiometric assay 14C-Substrates Amphotericin B Diethylstilbestrol Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Paracoccidioidomycosis (South American blastomycosis) is a systemic disease, strikingly more frequent in males, caused by the dimorphic fungus Paracoccidioides brasiliensis. A radiometric assay system has been applied to study the metabolic activity and the effect of drugs on this fungus "in vitro". The Y form of the yeast, grown in liquid Sabouraud medium was inoculated into sterile reaction vials containing the 6B aerobic medium along with 2.0 μCi of 14C-substrates. Control vials, prepared in the same way, contained autoclaved fungi. To study the effects of amphotericin B (AB) (0.1 and 10 μg/ml) and diethylstilbestrol (DSB) (1.0, 5.0 and 10 μg/ml) extra controls with live fungi and no drug were used. All vials were incubated at 35°C and metabolism measured daily with a Bactec instrument. 14CO2 production by P. brasiliensis was slow and could be followed for as long as 50 days. AB at 10mg/ml and DSB at 5 μg/ml inhibited the metabolism and had a cidal effect on this fungus. The results with DSB might explain the low incidence of the disease in females. This technique shows promise for studying metabolic pathways, investi gating more convenient 14C-substrates to expedite radiometric detection and for monitoring the effects of other drugs and factors on the metabolism of P. brasiliensis "in vitro". |
format |
Article in Journal/Newspaper |
author |
Edwaldo E. Camargo Maria K. Sato Gilda M. B. Del Negro Carlos da Silva Lacaz |
author_facet |
Edwaldo E. Camargo Maria K. Sato Gilda M. B. Del Negro Carlos da Silva Lacaz |
author_sort |
Edwaldo E. Camargo |
title |
Radiometric detection of metabolic activity of Paracoccidioides brasiliensis and its susceptibility to amphotericin B and diethylstilbestrol |
title_short |
Radiometric detection of metabolic activity of Paracoccidioides brasiliensis and its susceptibility to amphotericin B and diethylstilbestrol |
title_full |
Radiometric detection of metabolic activity of Paracoccidioides brasiliensis and its susceptibility to amphotericin B and diethylstilbestrol |
title_fullStr |
Radiometric detection of metabolic activity of Paracoccidioides brasiliensis and its susceptibility to amphotericin B and diethylstilbestrol |
title_full_unstemmed |
Radiometric detection of metabolic activity of Paracoccidioides brasiliensis and its susceptibility to amphotericin B and diethylstilbestrol |
title_sort |
radiometric detection of metabolic activity of paracoccidioides brasiliensis and its susceptibility to amphotericin b and diethylstilbestrol |
publisher |
Universidade de São Paulo (USP) |
publishDate |
1987 |
url |
https://doi.org/10.1590/S0036-46651987000500005 https://doaj.org/article/b104e73659b04bf7b585355a7ac3d306 |
long_lat |
ENVELOPE(-112.453,-112.453,57.591,57.591) |
geographic |
Arctic The ''Y'' |
geographic_facet |
Arctic The ''Y'' |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 29, Iss 5, Pp 289-294 (1987) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651987000500005&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46651987000500005 https://doaj.org/article/b104e73659b04bf7b585355a7ac3d306 |
op_doi |
https://doi.org/10.1590/S0036-46651987000500005 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
container_volume |
29 |
container_issue |
5 |
container_start_page |
289 |
op_container_end_page |
294 |
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1809896351059673088 |