Antarctic Krill Oil from Euphausia superba Ameliorates Carrageenan-Induced Thrombosis in a Mouse Model

• Published in: International Journal of Molecular Sciences
• Main Authors: Gi Ho Lee, Seung Yeon Lee, Ju Yeon Chae, Jae Won Kim, Jin-Hee Kim, Hye Gwang Jeong
• Format: Article in Journal/Newspaper
• Language: English
• Published: MDPI AG 2023
• Subjects: krill oil; carrageenan; thrombosis; inflammation; endothelial dysfunction; Biology (General); QH301-705.5; Chemistry; QD1-999; Antarctic; Antarc*; Antarctic Krill; Euphausia superba
• Online Access: https://doi.org/10.3390/ijms242417440; https://doaj.org/article/b102bca600c642c38e5027355409f269

FJH-KO obtained from Antarctic krill, especially Euphausia superba , has been reported to contain high amounts of omega-3 polyunsaturated fatty acids (n-3 PUFA) and to exhibit anticancer and anti-inflammatory properties. However, its antithrombotic effects have not yet been reported. This study aimed to investigate the antithrombotic effects of FJH-KO in carrageenan-induced thrombosis mouse models and human endothelial cells. Thrombosis was induced by carrageenan injection, whereas the mice received FJH-KO pretreatment. FJH-KO attenuated carrageenan-induced thrombus formation in mouse tissue vessels and prolonged tail bleeding. The inhibitory effect of FJH-KO was associated with decreased plasma levels of thromboxane B2, P-selectin, endothelin-1, β-thromboglobulin, platelet factor 4, serotonin, TNF-α, IL-1β, and IL-6. Meanwhile, FJH-KO induced plasma levels of prostacyclin I2 and plasminogen. In vitro, FJH-KO decreased the adhesion of THP-1 monocytes to human endothelial cells stimulated by TNF-α via eNOS activation and NO production. Furthermore, FJH-KO inhibited the expression of TNF-α-induced adhesion molecules such as ICAM-1 and VCAM-1 by suppressing the NF-κB signaling pathway. Taken together, our study demonstrates that FJH-KO protects against carrageenan-induced thrombosis by regulating endothelial cell activation and has potential as an antithrombotic agent.