Detection of Trypanosoma cruzi strains circulating in Córdoba department (Colombia) isolated from triatomines (Hemiptera: Reduviidae) collected by the community
Introduction: From 2011 to 2016, 24 cases of Chagas disease were reported in Córdoba according to the national public health surveillance system (Sistema Nacional de Vigilancia en Salud Pública, Sivigila), but the information regarding Trypanosoma cruzi circulating strains and infection rates are un...
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Instituto Nacional de Salud
2019
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ftdoajarticles:oai:doaj.org/article:b0c5c960514641d98703a0086427a398 2023-05-15T15:13:40+02:00 Detection of Trypanosoma cruzi strains circulating in Córdoba department (Colombia) isolated from triatomines (Hemiptera: Reduviidae) collected by the community Cielo León Mario Iván Ortiz Catalina Tovar Jorge Negrete Ernesto Arroyo Camila González 2019-06-01T00:00:00Z https://doi.org/10.7705/biomedica.v39i2.3973 https://doaj.org/article/b0c5c960514641d98703a0086427a398 EN ES eng spa Instituto Nacional de Salud https://www.revistabiomedica.org/index.php/biomedica/article/view/3973 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.v39i2.3973 https://doaj.org/article/b0c5c960514641d98703a0086427a398 Biomédica: revista del Instituto Nacional de Salud, Vol 39, Iss 2, Pp 265-277 (2019) Trypanosoma cruzi Chagas disease Rhodnius Panstrongylus Reduviidae Triatominae Medicine R Arctic medicine. Tropical medicine RC955-962 article 2019 ftdoajarticles https://doi.org/10.7705/biomedica.v39i2.3973 2022-12-30T23:44:59Z Introduction: From 2011 to 2016, 24 cases of Chagas disease were reported in Córdoba according to the national public health surveillance system (Sistema Nacional de Vigilancia en Salud Pública, Sivigila), but the information regarding Trypanosoma cruzi circulating strains and infection rates are unknown. Objectives: To establish the triatomine species with which people come in contact and recognize as Chagas disease vectors, as well as to assess the infection with trypanosomes and make an exploratory approach to host feeding preferences with the participation of the local community. Materials and methods: Triatomines sampling was conducted in 12 municipalities between 2011 and 2016; T. cruzi infection was established by k-PCR, SAT-PCR, while strain genotyping was done by mini-exon and SL-IR (spliced-leader intergenic region) sequence characterization. We also screened for blood sources. Results: Local community members collected the majority of triatomines and we identified three species: Rhodnius pallescens, Panstrongylus geniculatus, and Eratyrus cuspidatus. The overall T. cruzi infection rate in collected triatomines was 66.6% and we detected the TcIDOM and TcI sylvatic strains. Community-based insect collection allowed reporting the presence of P. geniculatus in two new disperse rural settlements, T. cruzi infection of P. geniculatus in Córdoba, and the first report of triatomines infected with T. cruzi in Montería municipality. Conclusions: These results revealed the presence of triatomines infected with T. cruzi inside dwellings in five municipalities of Córdoba. The dominant circulating T. cruzi strain was TcIDOM, a genotype associated with human Chagas disease and cardiomyopathies in Colombia. Our results highlight the importance of local community participation in entomological surveillance tasks. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Biomédica 39 2 265 277 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English Spanish |
topic |
Trypanosoma cruzi Chagas disease Rhodnius Panstrongylus Reduviidae Triatominae Medicine R Arctic medicine. Tropical medicine RC955-962 |
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Trypanosoma cruzi Chagas disease Rhodnius Panstrongylus Reduviidae Triatominae Medicine R Arctic medicine. Tropical medicine RC955-962 Cielo León Mario Iván Ortiz Catalina Tovar Jorge Negrete Ernesto Arroyo Camila González Detection of Trypanosoma cruzi strains circulating in Córdoba department (Colombia) isolated from triatomines (Hemiptera: Reduviidae) collected by the community |
topic_facet |
Trypanosoma cruzi Chagas disease Rhodnius Panstrongylus Reduviidae Triatominae Medicine R Arctic medicine. Tropical medicine RC955-962 |
description |
Introduction: From 2011 to 2016, 24 cases of Chagas disease were reported in Córdoba according to the national public health surveillance system (Sistema Nacional de Vigilancia en Salud Pública, Sivigila), but the information regarding Trypanosoma cruzi circulating strains and infection rates are unknown. Objectives: To establish the triatomine species with which people come in contact and recognize as Chagas disease vectors, as well as to assess the infection with trypanosomes and make an exploratory approach to host feeding preferences with the participation of the local community. Materials and methods: Triatomines sampling was conducted in 12 municipalities between 2011 and 2016; T. cruzi infection was established by k-PCR, SAT-PCR, while strain genotyping was done by mini-exon and SL-IR (spliced-leader intergenic region) sequence characterization. We also screened for blood sources. Results: Local community members collected the majority of triatomines and we identified three species: Rhodnius pallescens, Panstrongylus geniculatus, and Eratyrus cuspidatus. The overall T. cruzi infection rate in collected triatomines was 66.6% and we detected the TcIDOM and TcI sylvatic strains. Community-based insect collection allowed reporting the presence of P. geniculatus in two new disperse rural settlements, T. cruzi infection of P. geniculatus in Córdoba, and the first report of triatomines infected with T. cruzi in Montería municipality. Conclusions: These results revealed the presence of triatomines infected with T. cruzi inside dwellings in five municipalities of Córdoba. The dominant circulating T. cruzi strain was TcIDOM, a genotype associated with human Chagas disease and cardiomyopathies in Colombia. Our results highlight the importance of local community participation in entomological surveillance tasks. |
format |
Article in Journal/Newspaper |
author |
Cielo León Mario Iván Ortiz Catalina Tovar Jorge Negrete Ernesto Arroyo Camila González |
author_facet |
Cielo León Mario Iván Ortiz Catalina Tovar Jorge Negrete Ernesto Arroyo Camila González |
author_sort |
Cielo León |
title |
Detection of Trypanosoma cruzi strains circulating in Córdoba department (Colombia) isolated from triatomines (Hemiptera: Reduviidae) collected by the community |
title_short |
Detection of Trypanosoma cruzi strains circulating in Córdoba department (Colombia) isolated from triatomines (Hemiptera: Reduviidae) collected by the community |
title_full |
Detection of Trypanosoma cruzi strains circulating in Córdoba department (Colombia) isolated from triatomines (Hemiptera: Reduviidae) collected by the community |
title_fullStr |
Detection of Trypanosoma cruzi strains circulating in Córdoba department (Colombia) isolated from triatomines (Hemiptera: Reduviidae) collected by the community |
title_full_unstemmed |
Detection of Trypanosoma cruzi strains circulating in Córdoba department (Colombia) isolated from triatomines (Hemiptera: Reduviidae) collected by the community |
title_sort |
detection of trypanosoma cruzi strains circulating in córdoba department (colombia) isolated from triatomines (hemiptera: reduviidae) collected by the community |
publisher |
Instituto Nacional de Salud |
publishDate |
2019 |
url |
https://doi.org/10.7705/biomedica.v39i2.3973 https://doaj.org/article/b0c5c960514641d98703a0086427a398 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Biomédica: revista del Instituto Nacional de Salud, Vol 39, Iss 2, Pp 265-277 (2019) |
op_relation |
https://www.revistabiomedica.org/index.php/biomedica/article/view/3973 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.v39i2.3973 https://doaj.org/article/b0c5c960514641d98703a0086427a398 |
op_doi |
https://doi.org/10.7705/biomedica.v39i2.3973 |
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Biomédica |
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39 |
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2 |
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265 |
op_container_end_page |
277 |
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