Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas
Abstract This study investigated oyster infection dynamics by different strains of Vibrio aestuarianus isolated before and after the apparent re-emergence of this pathogen observed in France in 2011. We conducted experiments to compare minimal infective dose, lethal dose 50 and bacterial shedding fo...
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ftdoajarticles:oai:doaj.org/article:b0924b973b2b4cf883d8c33d7e00e3c7 2023-05-15T15:58:47+02:00 Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas Marie-Agnès Travers Delphine Tourbiez Leïla Parizadeh Philippe Haffner Angélique Kozic-Djellouli Mohamed Aboubaker Marcel Koken Lionel Dégremont Coralie Lupo 2017-05-01T00:00:00Z https://doi.org/10.1186/s13567-017-0438-1 https://doaj.org/article/b0924b973b2b4cf883d8c33d7e00e3c7 EN eng BMC http://link.springer.com/article/10.1186/s13567-017-0438-1 https://doaj.org/toc/1297-9716 doi:10.1186/s13567-017-0438-1 1297-9716 https://doaj.org/article/b0924b973b2b4cf883d8c33d7e00e3c7 Veterinary Research, Vol 48, Iss 1, Pp 1-8 (2017) Veterinary medicine SF600-1100 article 2017 ftdoajarticles https://doi.org/10.1186/s13567-017-0438-1 2022-12-31T14:41:12Z Abstract This study investigated oyster infection dynamics by different strains of Vibrio aestuarianus isolated before and after the apparent re-emergence of this pathogen observed in France in 2011. We conducted experiments to compare minimal infective dose, lethal dose 50 and bacterial shedding for six V. aestuarianus strains. Whatever the strain used, mortality was induced in juvenile oysters by intramuscular injection and reached 90–100% of mortality within 5 days. Moreover, bacterial shedding was comparable among strains and reached its maximum after 20 h (≈10 EXP5 bacteria/mL/animal). Similarly, our first estimations of lethal dose 50 were comparable among strains (minimal infective dose around 0.4 × 10EXP5 bacteria/mL and LD50 around 10EXP5 bacteria/mL) by using seawater containing freshly shed bacteria. These results indicate that, at least with these criteria, despite V. aestuarianus strains genetic diversity, the disease process is similar. The strains isolated after the apparent re-emergence of the bacteria in 2011, do not present a more acute virulence phenotype than the reference strains isolated between 2002 and 2007. Finally, our study provides original and noteworthy data indicating that infected oysters shed bacteria at a level above the threshold of LD50 a few days before they die, meaning that infection is expected to spread in a susceptible population. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Directory of Open Access Journals: DOAJ Articles Pacific Veterinary Research 48 1 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
Veterinary medicine SF600-1100 |
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Veterinary medicine SF600-1100 Marie-Agnès Travers Delphine Tourbiez Leïla Parizadeh Philippe Haffner Angélique Kozic-Djellouli Mohamed Aboubaker Marcel Koken Lionel Dégremont Coralie Lupo Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas |
topic_facet |
Veterinary medicine SF600-1100 |
description |
Abstract This study investigated oyster infection dynamics by different strains of Vibrio aestuarianus isolated before and after the apparent re-emergence of this pathogen observed in France in 2011. We conducted experiments to compare minimal infective dose, lethal dose 50 and bacterial shedding for six V. aestuarianus strains. Whatever the strain used, mortality was induced in juvenile oysters by intramuscular injection and reached 90–100% of mortality within 5 days. Moreover, bacterial shedding was comparable among strains and reached its maximum after 20 h (≈10 EXP5 bacteria/mL/animal). Similarly, our first estimations of lethal dose 50 were comparable among strains (minimal infective dose around 0.4 × 10EXP5 bacteria/mL and LD50 around 10EXP5 bacteria/mL) by using seawater containing freshly shed bacteria. These results indicate that, at least with these criteria, despite V. aestuarianus strains genetic diversity, the disease process is similar. The strains isolated after the apparent re-emergence of the bacteria in 2011, do not present a more acute virulence phenotype than the reference strains isolated between 2002 and 2007. Finally, our study provides original and noteworthy data indicating that infected oysters shed bacteria at a level above the threshold of LD50 a few days before they die, meaning that infection is expected to spread in a susceptible population. |
format |
Article in Journal/Newspaper |
author |
Marie-Agnès Travers Delphine Tourbiez Leïla Parizadeh Philippe Haffner Angélique Kozic-Djellouli Mohamed Aboubaker Marcel Koken Lionel Dégremont Coralie Lupo |
author_facet |
Marie-Agnès Travers Delphine Tourbiez Leïla Parizadeh Philippe Haffner Angélique Kozic-Djellouli Mohamed Aboubaker Marcel Koken Lionel Dégremont Coralie Lupo |
author_sort |
Marie-Agnès Travers |
title |
Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas |
title_short |
Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas |
title_full |
Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas |
title_fullStr |
Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas |
title_full_unstemmed |
Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas |
title_sort |
several strains, one disease: experimental investigation of vibrio aestuarianus infection parameters in the pacific oyster, crassostrea gigas |
publisher |
BMC |
publishDate |
2017 |
url |
https://doi.org/10.1186/s13567-017-0438-1 https://doaj.org/article/b0924b973b2b4cf883d8c33d7e00e3c7 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Veterinary Research, Vol 48, Iss 1, Pp 1-8 (2017) |
op_relation |
http://link.springer.com/article/10.1186/s13567-017-0438-1 https://doaj.org/toc/1297-9716 doi:10.1186/s13567-017-0438-1 1297-9716 https://doaj.org/article/b0924b973b2b4cf883d8c33d7e00e3c7 |
op_doi |
https://doi.org/10.1186/s13567-017-0438-1 |
container_title |
Veterinary Research |
container_volume |
48 |
container_issue |
1 |
_version_ |
1766394554061160448 |