Zika virus infection drives epigenetic modulation of immunity by the histone acetyltransferase CBP of Aedes aegypti.
Epigenetic mechanisms are responsible for a wide range of biological phenomena in insects, controlling embryonic development, growth, aging and nutrition. Despite this, the role of epigenetics in shaping insect-pathogen interactions has received little attention. Gene expression in eukaryotes is reg...
Published in: | PLOS Neglected Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:b030b789b8474bfe9cddcf9ecedc7fce 2023-05-15T15:07:16+02:00 Zika virus infection drives epigenetic modulation of immunity by the histone acetyltransferase CBP of Aedes aegypti. Anderson de Mendonça Amarante Isabel Caetano de Abreu da Silva Vitor Coutinho Carneiro Amanda Roberta Revoredo Vicentino Marcia de Amorim Pinto Luiza Mendonça Higa Kanhu Charan Moharana Octavio A C Talyuli Thiago Motta Venancio Pedro Lagerblad de Oliveira Marcelo Rosado Fantappié 2022-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0010559 https://doaj.org/article/b030b789b8474bfe9cddcf9ecedc7fce EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0010559 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010559 https://doaj.org/article/b030b789b8474bfe9cddcf9ecedc7fce PLoS Neglected Tropical Diseases, Vol 16, Iss 6, p e0010559 (2022) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2022 ftdoajarticles https://doi.org/10.1371/journal.pntd.0010559 2022-12-31T01:05:35Z Epigenetic mechanisms are responsible for a wide range of biological phenomena in insects, controlling embryonic development, growth, aging and nutrition. Despite this, the role of epigenetics in shaping insect-pathogen interactions has received little attention. Gene expression in eukaryotes is regulated by histone acetylation/deacetylation, an epigenetic process mediated by histone acetyltransferases (HATs) and histone deacetylases (HDACs). In this study, we explored the role of the Aedes aegypti histone acetyltransferase CBP (AaCBP) after infection with Zika virus (ZIKV), focusing on the two main immune tissues, the midgut and fat body. We showed that the expression and activity of AaCBP could be positively modulated by blood meal and ZIKV infection. Nevertheless, Zika-infected mosquitoes that were silenced for AaCBP revealed a significant reduction in the acetylation of H3K27 (CBP target marker), followed by downmodulation of the expression of immune genes, higher titers of ZIKV and lower survival rates. Importantly, in Zika-infected mosquitoes that were treated with sodium butyrate, a histone deacetylase inhibitor, their capacity to fight virus infection was rescued. Our data point to a direct correlation among histone hyperacetylation by AaCBP, upregulation of antimicrobial peptide genes and increased survival of Zika-infected-A. aegypti. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 16 6 e0010559 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Anderson de Mendonça Amarante Isabel Caetano de Abreu da Silva Vitor Coutinho Carneiro Amanda Roberta Revoredo Vicentino Marcia de Amorim Pinto Luiza Mendonça Higa Kanhu Charan Moharana Octavio A C Talyuli Thiago Motta Venancio Pedro Lagerblad de Oliveira Marcelo Rosado Fantappié Zika virus infection drives epigenetic modulation of immunity by the histone acetyltransferase CBP of Aedes aegypti. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Epigenetic mechanisms are responsible for a wide range of biological phenomena in insects, controlling embryonic development, growth, aging and nutrition. Despite this, the role of epigenetics in shaping insect-pathogen interactions has received little attention. Gene expression in eukaryotes is regulated by histone acetylation/deacetylation, an epigenetic process mediated by histone acetyltransferases (HATs) and histone deacetylases (HDACs). In this study, we explored the role of the Aedes aegypti histone acetyltransferase CBP (AaCBP) after infection with Zika virus (ZIKV), focusing on the two main immune tissues, the midgut and fat body. We showed that the expression and activity of AaCBP could be positively modulated by blood meal and ZIKV infection. Nevertheless, Zika-infected mosquitoes that were silenced for AaCBP revealed a significant reduction in the acetylation of H3K27 (CBP target marker), followed by downmodulation of the expression of immune genes, higher titers of ZIKV and lower survival rates. Importantly, in Zika-infected mosquitoes that were treated with sodium butyrate, a histone deacetylase inhibitor, their capacity to fight virus infection was rescued. Our data point to a direct correlation among histone hyperacetylation by AaCBP, upregulation of antimicrobial peptide genes and increased survival of Zika-infected-A. aegypti. |
format |
Article in Journal/Newspaper |
author |
Anderson de Mendonça Amarante Isabel Caetano de Abreu da Silva Vitor Coutinho Carneiro Amanda Roberta Revoredo Vicentino Marcia de Amorim Pinto Luiza Mendonça Higa Kanhu Charan Moharana Octavio A C Talyuli Thiago Motta Venancio Pedro Lagerblad de Oliveira Marcelo Rosado Fantappié |
author_facet |
Anderson de Mendonça Amarante Isabel Caetano de Abreu da Silva Vitor Coutinho Carneiro Amanda Roberta Revoredo Vicentino Marcia de Amorim Pinto Luiza Mendonça Higa Kanhu Charan Moharana Octavio A C Talyuli Thiago Motta Venancio Pedro Lagerblad de Oliveira Marcelo Rosado Fantappié |
author_sort |
Anderson de Mendonça Amarante |
title |
Zika virus infection drives epigenetic modulation of immunity by the histone acetyltransferase CBP of Aedes aegypti. |
title_short |
Zika virus infection drives epigenetic modulation of immunity by the histone acetyltransferase CBP of Aedes aegypti. |
title_full |
Zika virus infection drives epigenetic modulation of immunity by the histone acetyltransferase CBP of Aedes aegypti. |
title_fullStr |
Zika virus infection drives epigenetic modulation of immunity by the histone acetyltransferase CBP of Aedes aegypti. |
title_full_unstemmed |
Zika virus infection drives epigenetic modulation of immunity by the histone acetyltransferase CBP of Aedes aegypti. |
title_sort |
zika virus infection drives epigenetic modulation of immunity by the histone acetyltransferase cbp of aedes aegypti. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2022 |
url |
https://doi.org/10.1371/journal.pntd.0010559 https://doaj.org/article/b030b789b8474bfe9cddcf9ecedc7fce |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 16, Iss 6, p e0010559 (2022) |
op_relation |
https://doi.org/10.1371/journal.pntd.0010559 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010559 https://doaj.org/article/b030b789b8474bfe9cddcf9ecedc7fce |
op_doi |
https://doi.org/10.1371/journal.pntd.0010559 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
16 |
container_issue |
6 |
container_start_page |
e0010559 |
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1766338803693256704 |