Species-Specific Serological Detection for Schistosomiasis by Serine Protease Inhibitor (SERPIN) in Multiplex Assay.
Both Schistosoma mansoni and Schistosoma haematobium cause schistosomiasis in sub-Saharan Africa. We assessed the diagnostic value of selected Schistosoma antigens for the development of a multiplex serological immunoassay for sero-epidemiological surveillance.Diagnostic ability of recombinant antig...
Published in: | PLOS Neglected Tropical Diseases |
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2015
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Online Access: | https://doi.org/10.1371/journal.pntd.0004021 https://doaj.org/article/afe92342173147f4bf4fbd39f2f83f0f |
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ftdoajarticles:oai:doaj.org/article:afe92342173147f4bf4fbd39f2f83f0f 2023-05-15T15:11:11+02:00 Species-Specific Serological Detection for Schistosomiasis by Serine Protease Inhibitor (SERPIN) in Multiplex Assay. Chihiro Tanigawa Yoshito Fujii Masashi Miura Samson Muuo Nzou Anne Wanjiru Mwangi Sachiyo Nagi Shinjiro Hamano Sammy M Njenga Evaristus Chibunna Mbanefo Kenji Hirayama Matilu Mwau Satoshi Kaneko 2015-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0004021 https://doaj.org/article/afe92342173147f4bf4fbd39f2f83f0f EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4546333?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004021 https://doaj.org/article/afe92342173147f4bf4fbd39f2f83f0f PLoS Neglected Tropical Diseases, Vol 9, Iss 8, p e0004021 (2015) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2015 ftdoajarticles https://doi.org/10.1371/journal.pntd.0004021 2022-12-31T12:21:00Z Both Schistosoma mansoni and Schistosoma haematobium cause schistosomiasis in sub-Saharan Africa. We assessed the diagnostic value of selected Schistosoma antigens for the development of a multiplex serological immunoassay for sero-epidemiological surveillance.Diagnostic ability of recombinant antigens from S. mansoni and S. haematobium was assessed by Luminex multiplex immunoassay using plasma from school children in two areas of Kenya, endemic for different species of schistosomiasis. S. mansoni serine protease inhibitor (SERPIN) and Sm-RP26 showed significantly higher reactivity to patient plasma as compared to the control group. Sm-Filamin, Sm-GAPDH, Sm-GST, Sm-LAP1, Sm-LAP2, Sm-Sm31, Sm-Sm32 and Sm-Tropomyosin did not show difference in reactivity between S. mansoni infected and uninfected pupils. Sm-RP26 was cross-reactive to plasma from S. haematobium patients, whereas Sm-SERPIN was species-specific. Sh-SEPRIN was partially cross-reactive to S. mansoni infected patients. ROC analysis for Sm-RP26, Sm-SERPIN and Sh-SERPIN showed AUC values of 0.833, 0.888 and 0.947, respectively. Using Spearman's rank correlation coefficient analysis, we also found significant positive correlation between the number of excreted eggs and median fluorescence intensity (MFI) from the multiplex immunoassays for Sm-SERPIN (ρ = 0.430, p-value = 0.003) and Sh-SERPIN (ρ = 0.433, p-value = 0.006).Sm-SERPIN is a promising species-specific diagnostic antigen. Sh-SEPRIN was partially cross-reactive to S. mansoni infected patients. SERPINs showed correlation with the number of excreted eggs. These indicate prospects for inclusion of SERPINs in the multiplex serological immunoassay system. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 9 8 e0004021 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Chihiro Tanigawa Yoshito Fujii Masashi Miura Samson Muuo Nzou Anne Wanjiru Mwangi Sachiyo Nagi Shinjiro Hamano Sammy M Njenga Evaristus Chibunna Mbanefo Kenji Hirayama Matilu Mwau Satoshi Kaneko Species-Specific Serological Detection for Schistosomiasis by Serine Protease Inhibitor (SERPIN) in Multiplex Assay. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Both Schistosoma mansoni and Schistosoma haematobium cause schistosomiasis in sub-Saharan Africa. We assessed the diagnostic value of selected Schistosoma antigens for the development of a multiplex serological immunoassay for sero-epidemiological surveillance.Diagnostic ability of recombinant antigens from S. mansoni and S. haematobium was assessed by Luminex multiplex immunoassay using plasma from school children in two areas of Kenya, endemic for different species of schistosomiasis. S. mansoni serine protease inhibitor (SERPIN) and Sm-RP26 showed significantly higher reactivity to patient plasma as compared to the control group. Sm-Filamin, Sm-GAPDH, Sm-GST, Sm-LAP1, Sm-LAP2, Sm-Sm31, Sm-Sm32 and Sm-Tropomyosin did not show difference in reactivity between S. mansoni infected and uninfected pupils. Sm-RP26 was cross-reactive to plasma from S. haematobium patients, whereas Sm-SERPIN was species-specific. Sh-SEPRIN was partially cross-reactive to S. mansoni infected patients. ROC analysis for Sm-RP26, Sm-SERPIN and Sh-SERPIN showed AUC values of 0.833, 0.888 and 0.947, respectively. Using Spearman's rank correlation coefficient analysis, we also found significant positive correlation between the number of excreted eggs and median fluorescence intensity (MFI) from the multiplex immunoassays for Sm-SERPIN (ρ = 0.430, p-value = 0.003) and Sh-SERPIN (ρ = 0.433, p-value = 0.006).Sm-SERPIN is a promising species-specific diagnostic antigen. Sh-SEPRIN was partially cross-reactive to S. mansoni infected patients. SERPINs showed correlation with the number of excreted eggs. These indicate prospects for inclusion of SERPINs in the multiplex serological immunoassay system. |
format |
Article in Journal/Newspaper |
author |
Chihiro Tanigawa Yoshito Fujii Masashi Miura Samson Muuo Nzou Anne Wanjiru Mwangi Sachiyo Nagi Shinjiro Hamano Sammy M Njenga Evaristus Chibunna Mbanefo Kenji Hirayama Matilu Mwau Satoshi Kaneko |
author_facet |
Chihiro Tanigawa Yoshito Fujii Masashi Miura Samson Muuo Nzou Anne Wanjiru Mwangi Sachiyo Nagi Shinjiro Hamano Sammy M Njenga Evaristus Chibunna Mbanefo Kenji Hirayama Matilu Mwau Satoshi Kaneko |
author_sort |
Chihiro Tanigawa |
title |
Species-Specific Serological Detection for Schistosomiasis by Serine Protease Inhibitor (SERPIN) in Multiplex Assay. |
title_short |
Species-Specific Serological Detection for Schistosomiasis by Serine Protease Inhibitor (SERPIN) in Multiplex Assay. |
title_full |
Species-Specific Serological Detection for Schistosomiasis by Serine Protease Inhibitor (SERPIN) in Multiplex Assay. |
title_fullStr |
Species-Specific Serological Detection for Schistosomiasis by Serine Protease Inhibitor (SERPIN) in Multiplex Assay. |
title_full_unstemmed |
Species-Specific Serological Detection for Schistosomiasis by Serine Protease Inhibitor (SERPIN) in Multiplex Assay. |
title_sort |
species-specific serological detection for schistosomiasis by serine protease inhibitor (serpin) in multiplex assay. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pntd.0004021 https://doaj.org/article/afe92342173147f4bf4fbd39f2f83f0f |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 9, Iss 8, p e0004021 (2015) |
op_relation |
http://europepmc.org/articles/PMC4546333?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004021 https://doaj.org/article/afe92342173147f4bf4fbd39f2f83f0f |
op_doi |
https://doi.org/10.1371/journal.pntd.0004021 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
9 |
container_issue |
8 |
container_start_page |
e0004021 |
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1766342087355138048 |