Astrovirus induced nonpurulent encephalomyelitis in sheep: First report from Türkiye by high‐throughput sequencing

• Published in: Veterinary Medicine and Science
• Main Authors: Yesari Eroksuz, Mehmet Ozkan Timurkan, Farzane Shams, Torsten Seuberlich, Burak Karabulut, Canan Akdeniz Incili, Emel Kara, Hatice Eroksuz
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2024
• Subjects: astrovirus; encephalitis; high‐throughput sequencing; immunohistochemistry; non‐purulent encephalomyelitiss; Veterinary medicine; SF600-1100; muskox
• Online Access: https://doi.org/10.1002/vms3.1499; https://doaj.org/article/afdd8f8c09944e6ebc60420d480484b8

Abstract Background This study presents the case of non‐purulent encephalomyelitis associated with astrovirus infection in a sheep from Eastern Anatolia, Türkiye. Methods A necropsy was performed on a sheep showing nervous signs. Afterwards, brain tissue samples were taken and examined with histopathological, immunohistochemical and molecular techniques. Results Neuropathologic changes included neuronal degeneration, diffuse gliosis, multifocal perivascular cuffing, neuronophagy and neuronal necrosis in the cerebrum, the cerebellum and the cervical spinal cord. Aerobic and anaerobic bacterial culture, selective culture for Listeria monocytogenes, and PCR analysis for rabies virus, tick‐borne encephalitis virus, Türkiye encephalitis virus, small ruminant lentiviruses and border disease virus were negative. However, the presence of astrovirus RNA in cerebral, cerebellar and spinal cord samples was demonstrated by a pan‐astrovirus RT‐PCR. Immunohistochemical examinations revealed astrovirus antigens within the neuronal cytoplasm. High‐throughput sequencing techniques identified the causative agent as a member of the genotype species Mamastrovirus 13 but representing a distinct genetic lineage with similarity to ovine astrovirus 1 in the open‐reading frames (ORF)1ab region and muskox astrovirus in the ORF2 region. Conclusion This report provides evidence that astroviruses are potentially encephalitis‐causing pathogens in ovine populations in Türkiye, featuring an astrovirus strain distinct from those previously identified in sheep.