A high infectious simian adenovirus type 23 vector based vaccine efficiently protects common marmosets against Zika virus infection.
Zika virus (ZIKV) has spread in many countries or territories causing severe neurologic complications with potential fatal outcomes. The small primate common marmosets are susceptible to ZIKV, mimicking key features of human infection. Here, a novel simian adenovirus type 23 vector-based vaccine exp...
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Online Access: | https://doi.org/10.1371/journal.pntd.0008027 https://doaj.org/article/afb36d0bed1242518ac9f8ca33dd8313 |
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ftdoajarticles:oai:doaj.org/article:afb36d0bed1242518ac9f8ca33dd8313 2023-05-15T15:12:21+02:00 A high infectious simian adenovirus type 23 vector based vaccine efficiently protects common marmosets against Zika virus infection. Shengxue Luo Wei Zhao Xiaorui Ma Panli Zhang Bochao Liu Ling Zhang Wenjing Wang Yuanzhan Wang Yongshui Fu Jean-Pierre Allain Tingting Li Chengyao Li 2020-02-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008027 https://doaj.org/article/afb36d0bed1242518ac9f8ca33dd8313 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008027 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008027 https://doaj.org/article/afb36d0bed1242518ac9f8ca33dd8313 PLoS Neglected Tropical Diseases, Vol 14, Iss 2, p e0008027 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008027 2022-12-31T05:51:59Z Zika virus (ZIKV) has spread in many countries or territories causing severe neurologic complications with potential fatal outcomes. The small primate common marmosets are susceptible to ZIKV, mimicking key features of human infection. Here, a novel simian adenovirus type 23 vector-based vaccine expressing ZIKV pre-membrane-envelope proteins (Sad23L-prM-E) was produced in high infectious titer. Due to determination of immunogenicity in mice, a single-dose of 3×108 PFU Sad23L-prM-E vaccine was intramuscularly inoculated to marmosets. This vaccine raised antibody titers of 104.07 E-specific and 103.13 neutralizing antibody (NAb), as well as robust specific IFN-γ secreting T-cell response (1,219 SFCs/106 cells) to E peptides. The vaccinated marmosets, upon challenge with a high dose of ZIKV (105 PFU) six weeks post prime immunization, reduced viremia by more than 100 folds, and the low level of detectable viral RNA (<103 copies/ml) in blood, saliva, urine and feces was promptly eliminated when the secondary NAb (titer >103.66) and T-cell response (>726 SFCs/106 PBMCs) were acquired 1-2 weeks post exposure to ZIKV, while non-vaccinated control marmosets developed long-term high titer of ZIKV (105.73 copies/ml) (P<0.05). No significant pathological lesions were observed in marmoset tissues. Sad23L-prM-E vaccine was detectable in spleen, liver and PBMCs at least 4 months post challenge. In conclusion, a prime immunization with Sad23L-prM-E vaccine was able to protect marmosets against ZIKV infection when exposed to a high dose of ZIKV. This Sad23L-prM-E vaccine is a promising vaccine candidate for prevention of ZIKV infection in humans. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 2 e0008027 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Shengxue Luo Wei Zhao Xiaorui Ma Panli Zhang Bochao Liu Ling Zhang Wenjing Wang Yuanzhan Wang Yongshui Fu Jean-Pierre Allain Tingting Li Chengyao Li A high infectious simian adenovirus type 23 vector based vaccine efficiently protects common marmosets against Zika virus infection. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Zika virus (ZIKV) has spread in many countries or territories causing severe neurologic complications with potential fatal outcomes. The small primate common marmosets are susceptible to ZIKV, mimicking key features of human infection. Here, a novel simian adenovirus type 23 vector-based vaccine expressing ZIKV pre-membrane-envelope proteins (Sad23L-prM-E) was produced in high infectious titer. Due to determination of immunogenicity in mice, a single-dose of 3×108 PFU Sad23L-prM-E vaccine was intramuscularly inoculated to marmosets. This vaccine raised antibody titers of 104.07 E-specific and 103.13 neutralizing antibody (NAb), as well as robust specific IFN-γ secreting T-cell response (1,219 SFCs/106 cells) to E peptides. The vaccinated marmosets, upon challenge with a high dose of ZIKV (105 PFU) six weeks post prime immunization, reduced viremia by more than 100 folds, and the low level of detectable viral RNA (<103 copies/ml) in blood, saliva, urine and feces was promptly eliminated when the secondary NAb (titer >103.66) and T-cell response (>726 SFCs/106 PBMCs) were acquired 1-2 weeks post exposure to ZIKV, while non-vaccinated control marmosets developed long-term high titer of ZIKV (105.73 copies/ml) (P<0.05). No significant pathological lesions were observed in marmoset tissues. Sad23L-prM-E vaccine was detectable in spleen, liver and PBMCs at least 4 months post challenge. In conclusion, a prime immunization with Sad23L-prM-E vaccine was able to protect marmosets against ZIKV infection when exposed to a high dose of ZIKV. This Sad23L-prM-E vaccine is a promising vaccine candidate for prevention of ZIKV infection in humans. |
format |
Article in Journal/Newspaper |
author |
Shengxue Luo Wei Zhao Xiaorui Ma Panli Zhang Bochao Liu Ling Zhang Wenjing Wang Yuanzhan Wang Yongshui Fu Jean-Pierre Allain Tingting Li Chengyao Li |
author_facet |
Shengxue Luo Wei Zhao Xiaorui Ma Panli Zhang Bochao Liu Ling Zhang Wenjing Wang Yuanzhan Wang Yongshui Fu Jean-Pierre Allain Tingting Li Chengyao Li |
author_sort |
Shengxue Luo |
title |
A high infectious simian adenovirus type 23 vector based vaccine efficiently protects common marmosets against Zika virus infection. |
title_short |
A high infectious simian adenovirus type 23 vector based vaccine efficiently protects common marmosets against Zika virus infection. |
title_full |
A high infectious simian adenovirus type 23 vector based vaccine efficiently protects common marmosets against Zika virus infection. |
title_fullStr |
A high infectious simian adenovirus type 23 vector based vaccine efficiently protects common marmosets against Zika virus infection. |
title_full_unstemmed |
A high infectious simian adenovirus type 23 vector based vaccine efficiently protects common marmosets against Zika virus infection. |
title_sort |
high infectious simian adenovirus type 23 vector based vaccine efficiently protects common marmosets against zika virus infection. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008027 https://doaj.org/article/afb36d0bed1242518ac9f8ca33dd8313 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 2, p e0008027 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008027 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008027 https://doaj.org/article/afb36d0bed1242518ac9f8ca33dd8313 |
op_doi |
https://doi.org/10.1371/journal.pntd.0008027 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
14 |
container_issue |
2 |
container_start_page |
e0008027 |
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1766343044233166848 |