Dynamics of DNA methylomes underlie oyster development.
DNA methylation is a critical epigenetic regulator of development in mammals and social insects, but its significance in development outside these groups is not understood. Here we investigated the genome-wide dynamics of DNA methylation in a mollusc model, the oyster Crassostrea gigas, from the egg...
Published in: | PLOS Genetics |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2017
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pgen.1006807 https://doaj.org/article/afaa773cbf9241c593836cf31a1c6dff |
id |
ftdoajarticles:oai:doaj.org/article:afaa773cbf9241c593836cf31a1c6dff |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:afaa773cbf9241c593836cf31a1c6dff 2023-05-15T15:58:40+02:00 Dynamics of DNA methylomes underlie oyster development. Guillaume Riviere Yan He Samuele Tecchio Elizabeth Crowell Michaël Gras Pascal Sourdaine Ximing Guo Pascal Favrel 2017-06-01T00:00:00Z https://doi.org/10.1371/journal.pgen.1006807 https://doaj.org/article/afaa773cbf9241c593836cf31a1c6dff EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5481141?pdf=render https://doaj.org/toc/1553-7390 https://doaj.org/toc/1553-7404 1553-7390 1553-7404 doi:10.1371/journal.pgen.1006807 https://doaj.org/article/afaa773cbf9241c593836cf31a1c6dff PLoS Genetics, Vol 13, Iss 6, p e1006807 (2017) Genetics QH426-470 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pgen.1006807 2022-12-31T10:55:57Z DNA methylation is a critical epigenetic regulator of development in mammals and social insects, but its significance in development outside these groups is not understood. Here we investigated the genome-wide dynamics of DNA methylation in a mollusc model, the oyster Crassostrea gigas, from the egg to the completion of organogenesis. Large-scale methylation maps reveal that the oyster genome displays a succession of methylated and non methylated regions, which persist throughout development. Differentially methylated regions (DMRs) are strongly regulated during cleavage and metamorphosis. The distribution and levels of methylated DNA within genomic features (exons, introns, promoters, repeats and transposons) show different developmental lansdscapes marked by a strong increase in the methylation of exons against introns after metamorphosis. Kinetics of methylation in gene-bodies correlate to their transcription regulation and to distinct functional gene clusters, and DMRs at cleavage and metamorphosis bear the genes functionally related to these steps, respectively. This study shows that DNA methylome dynamics underlie development through transcription regulation in the oyster, a lophotrochozoan species. To our knowledge, this is the first demonstration of such epigenetic regulation outside vertebrates and ecdysozoan models, bringing new insights into the evolution and the epigenetic regulation of developmental processes. Article in Journal/Newspaper Crassostrea gigas Directory of Open Access Journals: DOAJ Articles PLOS Genetics 13 6 e1006807 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Genetics QH426-470 |
spellingShingle |
Genetics QH426-470 Guillaume Riviere Yan He Samuele Tecchio Elizabeth Crowell Michaël Gras Pascal Sourdaine Ximing Guo Pascal Favrel Dynamics of DNA methylomes underlie oyster development. |
topic_facet |
Genetics QH426-470 |
description |
DNA methylation is a critical epigenetic regulator of development in mammals and social insects, but its significance in development outside these groups is not understood. Here we investigated the genome-wide dynamics of DNA methylation in a mollusc model, the oyster Crassostrea gigas, from the egg to the completion of organogenesis. Large-scale methylation maps reveal that the oyster genome displays a succession of methylated and non methylated regions, which persist throughout development. Differentially methylated regions (DMRs) are strongly regulated during cleavage and metamorphosis. The distribution and levels of methylated DNA within genomic features (exons, introns, promoters, repeats and transposons) show different developmental lansdscapes marked by a strong increase in the methylation of exons against introns after metamorphosis. Kinetics of methylation in gene-bodies correlate to their transcription regulation and to distinct functional gene clusters, and DMRs at cleavage and metamorphosis bear the genes functionally related to these steps, respectively. This study shows that DNA methylome dynamics underlie development through transcription regulation in the oyster, a lophotrochozoan species. To our knowledge, this is the first demonstration of such epigenetic regulation outside vertebrates and ecdysozoan models, bringing new insights into the evolution and the epigenetic regulation of developmental processes. |
format |
Article in Journal/Newspaper |
author |
Guillaume Riviere Yan He Samuele Tecchio Elizabeth Crowell Michaël Gras Pascal Sourdaine Ximing Guo Pascal Favrel |
author_facet |
Guillaume Riviere Yan He Samuele Tecchio Elizabeth Crowell Michaël Gras Pascal Sourdaine Ximing Guo Pascal Favrel |
author_sort |
Guillaume Riviere |
title |
Dynamics of DNA methylomes underlie oyster development. |
title_short |
Dynamics of DNA methylomes underlie oyster development. |
title_full |
Dynamics of DNA methylomes underlie oyster development. |
title_fullStr |
Dynamics of DNA methylomes underlie oyster development. |
title_full_unstemmed |
Dynamics of DNA methylomes underlie oyster development. |
title_sort |
dynamics of dna methylomes underlie oyster development. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pgen.1006807 https://doaj.org/article/afaa773cbf9241c593836cf31a1c6dff |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
PLoS Genetics, Vol 13, Iss 6, p e1006807 (2017) |
op_relation |
http://europepmc.org/articles/PMC5481141?pdf=render https://doaj.org/toc/1553-7390 https://doaj.org/toc/1553-7404 1553-7390 1553-7404 doi:10.1371/journal.pgen.1006807 https://doaj.org/article/afaa773cbf9241c593836cf31a1c6dff |
op_doi |
https://doi.org/10.1371/journal.pgen.1006807 |
container_title |
PLOS Genetics |
container_volume |
13 |
container_issue |
6 |
container_start_page |
e1006807 |
_version_ |
1766394444697829376 |