Evaluation of l-dopa, proximate composition with in vitro anti-inflammatory and antioxidant activity of Mucuna macrocarpa beans: A future drug for Parkinson treatment
Objective: To investigate L-3, 4-dihydroxyphenylalanine (l-dopa, anti-Parkinson drug), anti-inflammatory activity, proximate nutritional composition and antioxidant potential of Mucuna macrocarpa (M. macrocarpa) beans. Methods: l-dopa content was determined and quantified by high performance thin la...
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Wolters Kluwer Medknow Publications
2017
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Online Access: | https://doi.org/10.1016/j.apjtb.2017.10.012 https://doaj.org/article/af4f6ae13d434eab8110a9fbb0bc9679 |
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ftdoajarticles:oai:doaj.org/article:af4f6ae13d434eab8110a9fbb0bc9679 2023-05-15T15:13:21+02:00 Evaluation of l-dopa, proximate composition with in vitro anti-inflammatory and antioxidant activity of Mucuna macrocarpa beans: A future drug for Parkinson treatment Chetan Aware Ravishankar Patil Swaroopsingh Gaikwad Shrirang Yadav Vishwas Bapat Jyoti Jadhav 2017-12-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2017.10.012 https://doaj.org/article/af4f6ae13d434eab8110a9fbb0bc9679 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169117309541 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2017.10.012 https://doaj.org/article/af4f6ae13d434eab8110a9fbb0bc9679 Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 12, Pp 1097-1106 (2017) Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2017 ftdoajarticles https://doi.org/10.1016/j.apjtb.2017.10.012 2022-12-31T03:23:51Z Objective: To investigate L-3, 4-dihydroxyphenylalanine (l-dopa, anti-Parkinson drug), anti-inflammatory activity, proximate nutritional composition and antioxidant potential of Mucuna macrocarpa (M. macrocarpa) beans. Methods: l-dopa content was determined and quantified by high performance thin layer chromatography and reversed phase high-performance liquid chromatography (RP-HPLC) methods. Anti-inflammatory activity was performed by in vitro protein denaturation inhibition and human red blood cell membrane stabilisation activity. Proximate composition and elemental analysis were also investigated. The antioxidant potential (2,2-diphenyl-1-picrylhydrazyl, N-N-dimethyl-phenylenediamine and ferric-reducing antioxidant power) of M. macrocarpa beans were evaluated by using different extraction solvents. The RP-HPLC analysis also quantified significant phenolics such as gallic acid, tannic acid, p-hydroxybenzoic acid and p-coumaric acid. Results: RP-HPLC quantification revealed that M. macrocarpa beans contain a high level of l-dopa [(115.41 ± 0.985) mg/g] which was the highest among the Mucuna species from Indian sub-continent. Water extract of seed powder showed strong anti-inflammatory and antioxidant potential. Proximate composition of M. macrocarpa beans revealed numerous nutritional and anti-nutritional components. RP-HPLC analysis of major phenolics such as tannic acid (43.795 mg/g), gallic acid (0.864 mg/g), p-coumaric acid (0.364 mg/g) and p-hydroxybenzoic acid (0.036 mg/g) quantified successfully from M. macrocarpa beans respectively. Conclusions: This study suggests that M. macrocarpa is a potential source of l-dopa with promising anti-inflammatory, antioxidant and nutritional benefits. Keywords: Anti-inflammatory, Antioxidants, l-dopa, Mucuna macrocarpa, Oxidative stress, Parkinson's disease Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Indian Asian Pacific Journal of Tropical Biomedicine 7 12 1097 1106 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Chetan Aware Ravishankar Patil Swaroopsingh Gaikwad Shrirang Yadav Vishwas Bapat Jyoti Jadhav Evaluation of l-dopa, proximate composition with in vitro anti-inflammatory and antioxidant activity of Mucuna macrocarpa beans: A future drug for Parkinson treatment |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate L-3, 4-dihydroxyphenylalanine (l-dopa, anti-Parkinson drug), anti-inflammatory activity, proximate nutritional composition and antioxidant potential of Mucuna macrocarpa (M. macrocarpa) beans. Methods: l-dopa content was determined and quantified by high performance thin layer chromatography and reversed phase high-performance liquid chromatography (RP-HPLC) methods. Anti-inflammatory activity was performed by in vitro protein denaturation inhibition and human red blood cell membrane stabilisation activity. Proximate composition and elemental analysis were also investigated. The antioxidant potential (2,2-diphenyl-1-picrylhydrazyl, N-N-dimethyl-phenylenediamine and ferric-reducing antioxidant power) of M. macrocarpa beans were evaluated by using different extraction solvents. The RP-HPLC analysis also quantified significant phenolics such as gallic acid, tannic acid, p-hydroxybenzoic acid and p-coumaric acid. Results: RP-HPLC quantification revealed that M. macrocarpa beans contain a high level of l-dopa [(115.41 ± 0.985) mg/g] which was the highest among the Mucuna species from Indian sub-continent. Water extract of seed powder showed strong anti-inflammatory and antioxidant potential. Proximate composition of M. macrocarpa beans revealed numerous nutritional and anti-nutritional components. RP-HPLC analysis of major phenolics such as tannic acid (43.795 mg/g), gallic acid (0.864 mg/g), p-coumaric acid (0.364 mg/g) and p-hydroxybenzoic acid (0.036 mg/g) quantified successfully from M. macrocarpa beans respectively. Conclusions: This study suggests that M. macrocarpa is a potential source of l-dopa with promising anti-inflammatory, antioxidant and nutritional benefits. Keywords: Anti-inflammatory, Antioxidants, l-dopa, Mucuna macrocarpa, Oxidative stress, Parkinson's disease |
format |
Article in Journal/Newspaper |
author |
Chetan Aware Ravishankar Patil Swaroopsingh Gaikwad Shrirang Yadav Vishwas Bapat Jyoti Jadhav |
author_facet |
Chetan Aware Ravishankar Patil Swaroopsingh Gaikwad Shrirang Yadav Vishwas Bapat Jyoti Jadhav |
author_sort |
Chetan Aware |
title |
Evaluation of l-dopa, proximate composition with in vitro anti-inflammatory and antioxidant activity of Mucuna macrocarpa beans: A future drug for Parkinson treatment |
title_short |
Evaluation of l-dopa, proximate composition with in vitro anti-inflammatory and antioxidant activity of Mucuna macrocarpa beans: A future drug for Parkinson treatment |
title_full |
Evaluation of l-dopa, proximate composition with in vitro anti-inflammatory and antioxidant activity of Mucuna macrocarpa beans: A future drug for Parkinson treatment |
title_fullStr |
Evaluation of l-dopa, proximate composition with in vitro anti-inflammatory and antioxidant activity of Mucuna macrocarpa beans: A future drug for Parkinson treatment |
title_full_unstemmed |
Evaluation of l-dopa, proximate composition with in vitro anti-inflammatory and antioxidant activity of Mucuna macrocarpa beans: A future drug for Parkinson treatment |
title_sort |
evaluation of l-dopa, proximate composition with in vitro anti-inflammatory and antioxidant activity of mucuna macrocarpa beans: a future drug for parkinson treatment |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2017 |
url |
https://doi.org/10.1016/j.apjtb.2017.10.012 https://doaj.org/article/af4f6ae13d434eab8110a9fbb0bc9679 |
geographic |
Arctic Indian |
geographic_facet |
Arctic Indian |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 12, Pp 1097-1106 (2017) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169117309541 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2017.10.012 https://doaj.org/article/af4f6ae13d434eab8110a9fbb0bc9679 |
op_doi |
https://doi.org/10.1016/j.apjtb.2017.10.012 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
7 |
container_issue |
12 |
container_start_page |
1097 |
op_container_end_page |
1106 |
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1766343919313879040 |