A novel beta-defensin antimicrobial peptide in Atlantic cod with stimulatory effect on phagocytic activity.
A novel defensin antimicrobial peptide gene was identified in Atlantic cod, Gadus morhua. This three exon/two intron defensin gene codes for a peptide precursor consisting of two domains: a signal peptide of 26 amino acids and a mature peptide of 40 residues. The mature cod defensin has six conserve...
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ftdoajarticles:oai:doaj.org/article:af38be5a43304b19a328b800b5b4c5b8 2023-05-15T15:27:04+02:00 A novel beta-defensin antimicrobial peptide in Atlantic cod with stimulatory effect on phagocytic activity. Jareeporn Ruangsri Yoichiro Kitani Viswanath Kiron Jep Lokesh Monica F Brinchmann Bård Ove Karlsen Jorge M O Fernandes 2013-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0062302 https://doaj.org/article/af38be5a43304b19a328b800b5b4c5b8 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3636224?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0062302 https://doaj.org/article/af38be5a43304b19a328b800b5b4c5b8 PLoS ONE, Vol 8, Iss 4, p e62302 (2013) Medicine R Science Q article 2013 ftdoajarticles https://doi.org/10.1371/journal.pone.0062302 2022-12-31T14:19:57Z A novel defensin antimicrobial peptide gene was identified in Atlantic cod, Gadus morhua. This three exon/two intron defensin gene codes for a peptide precursor consisting of two domains: a signal peptide of 26 amino acids and a mature peptide of 40 residues. The mature cod defensin has six conserved cysteine residues that form 1-5, 2-4 and 3-6 disulphide bridges. This pattern is typical of beta-defensins and this gene was therefore named cod beta-defensin (defb). The tertiary structure of Defb exhibits an α/β fold with one α helix and β1β2β3 sheets. RT-PCR analysis indicated that defb transcripts were present mainly in the swim bladder and peritoneum wall but could also be detected at moderate to low levels in skin, head- and excretory kidneys. In situ hybridisation revealed that defb was specifically expressed by cells located in the swim bladder submucosa and the oocytes. During embryonic development, defb gene transcripts were detectable from the golden eye stage onwards and their expression was restricted to the swim bladder and retina. Defb was differentially expressed in several tissues following antigenic challenge with Vibrio anguillarum, being up-regulated up to 25-fold in head kidney. Recombinant Defb displayed antibacterial activity, with a minimal inhibitory concentration of 0.4-0.8 µM and 25-50 µM against the Gram-(+) bacteria Planococcus citreus and Micrococcus luteus, respectively. In addition, Defb stimulated phagocytic activity of cod head kidney leucocytes in vitro. These findings imply that beta-defensins may play an important role in the innate immune response of Atlantic cod. Article in Journal/Newspaper atlantic cod Gadus morhua Directory of Open Access Journals: DOAJ Articles PLoS ONE 8 4 e62302 |
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Medicine R Science Q Jareeporn Ruangsri Yoichiro Kitani Viswanath Kiron Jep Lokesh Monica F Brinchmann Bård Ove Karlsen Jorge M O Fernandes A novel beta-defensin antimicrobial peptide in Atlantic cod with stimulatory effect on phagocytic activity. |
topic_facet |
Medicine R Science Q |
description |
A novel defensin antimicrobial peptide gene was identified in Atlantic cod, Gadus morhua. This three exon/two intron defensin gene codes for a peptide precursor consisting of two domains: a signal peptide of 26 amino acids and a mature peptide of 40 residues. The mature cod defensin has six conserved cysteine residues that form 1-5, 2-4 and 3-6 disulphide bridges. This pattern is typical of beta-defensins and this gene was therefore named cod beta-defensin (defb). The tertiary structure of Defb exhibits an α/β fold with one α helix and β1β2β3 sheets. RT-PCR analysis indicated that defb transcripts were present mainly in the swim bladder and peritoneum wall but could also be detected at moderate to low levels in skin, head- and excretory kidneys. In situ hybridisation revealed that defb was specifically expressed by cells located in the swim bladder submucosa and the oocytes. During embryonic development, defb gene transcripts were detectable from the golden eye stage onwards and their expression was restricted to the swim bladder and retina. Defb was differentially expressed in several tissues following antigenic challenge with Vibrio anguillarum, being up-regulated up to 25-fold in head kidney. Recombinant Defb displayed antibacterial activity, with a minimal inhibitory concentration of 0.4-0.8 µM and 25-50 µM against the Gram-(+) bacteria Planococcus citreus and Micrococcus luteus, respectively. In addition, Defb stimulated phagocytic activity of cod head kidney leucocytes in vitro. These findings imply that beta-defensins may play an important role in the innate immune response of Atlantic cod. |
format |
Article in Journal/Newspaper |
author |
Jareeporn Ruangsri Yoichiro Kitani Viswanath Kiron Jep Lokesh Monica F Brinchmann Bård Ove Karlsen Jorge M O Fernandes |
author_facet |
Jareeporn Ruangsri Yoichiro Kitani Viswanath Kiron Jep Lokesh Monica F Brinchmann Bård Ove Karlsen Jorge M O Fernandes |
author_sort |
Jareeporn Ruangsri |
title |
A novel beta-defensin antimicrobial peptide in Atlantic cod with stimulatory effect on phagocytic activity. |
title_short |
A novel beta-defensin antimicrobial peptide in Atlantic cod with stimulatory effect on phagocytic activity. |
title_full |
A novel beta-defensin antimicrobial peptide in Atlantic cod with stimulatory effect on phagocytic activity. |
title_fullStr |
A novel beta-defensin antimicrobial peptide in Atlantic cod with stimulatory effect on phagocytic activity. |
title_full_unstemmed |
A novel beta-defensin antimicrobial peptide in Atlantic cod with stimulatory effect on phagocytic activity. |
title_sort |
novel beta-defensin antimicrobial peptide in atlantic cod with stimulatory effect on phagocytic activity. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pone.0062302 https://doaj.org/article/af38be5a43304b19a328b800b5b4c5b8 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
PLoS ONE, Vol 8, Iss 4, p e62302 (2013) |
op_relation |
http://europepmc.org/articles/PMC3636224?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0062302 https://doaj.org/article/af38be5a43304b19a328b800b5b4c5b8 |
op_doi |
https://doi.org/10.1371/journal.pone.0062302 |
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PLoS ONE |
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8 |
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4 |
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e62302 |
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1766357533798170624 |