Affinity-Enhanced CTC-Capturing Hydrogel Microparticles Fabricated by Degassed Mold Lithography
Technologies for the detection and isolation of circulating tumor cells (CTCs) are essential in liquid biopsy, a minimally invasive technique for early diagnosis and medical intervention in cancer patients. A promising method for CTC capture, using an affinity-based approach, is the use of functiona...
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2020
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Online Access: | https://doi.org/10.3390/jcm9020301 https://doaj.org/article/af29e7f3c0194f05bf88910f6c2d8f61 |
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ftdoajarticles:oai:doaj.org/article:af29e7f3c0194f05bf88910f6c2d8f61 2023-05-15T16:01:29+02:00 Affinity-Enhanced CTC-Capturing Hydrogel Microparticles Fabricated by Degassed Mold Lithography Nak Jun Lee Sejung Maeng Hyeon Ung Kim Yoon Ho Roh Changhyun Hwang Jongjin Kim Ki-Tae Hwang Ki Wan Bong 2020-01-01T00:00:00Z https://doi.org/10.3390/jcm9020301 https://doaj.org/article/af29e7f3c0194f05bf88910f6c2d8f61 EN eng MDPI AG https://www.mdpi.com/2077-0383/9/2/301 https://doaj.org/toc/2077-0383 2077-0383 doi:10.3390/jcm9020301 https://doaj.org/article/af29e7f3c0194f05bf88910f6c2d8f61 Journal of Clinical Medicine, Vol 9, Iss 2, p 301 (2020) circulating tumor cell cell capture hydrogel microparticle degassed mold lithography Medicine R article 2020 ftdoajarticles https://doi.org/10.3390/jcm9020301 2022-12-31T03:22:30Z Technologies for the detection and isolation of circulating tumor cells (CTCs) are essential in liquid biopsy, a minimally invasive technique for early diagnosis and medical intervention in cancer patients. A promising method for CTC capture, using an affinity-based approach, is the use of functionalized hydrogel microparticles (MP), which have the advantages of water-like reactivity, biologically compatible materials, and synergy with various analysis platforms. In this paper, we demonstrate the feasibility of CTC capture by hydrogel particles synthesized using a novel method called degassed mold lithography (DML). This technique increases the porosity and functionality of the MPs for effective conjugation with antibodies. Qualitative fluorescence analysis demonstrates that DML produces superior uniformity, integrity, and functionality of the MPs, as compared to conventional stop flow lithography (SFL). Analysis of the fluorescence intensity from porosity-controlled MPs by each reaction step of antibody conjugation elucidates that more antibodies are loaded when the particles are more porous. The feasibility of selective cell capture is demonstrated using breast cancer cell lines. In conclusion, using DML for the synthesis of porous MPs offers a powerful method for improving the cell affinity of the antibody-conjugated MPs. Article in Journal/Newspaper DML Directory of Open Access Journals: DOAJ Articles Journal of Clinical Medicine 9 2 301 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
circulating tumor cell cell capture hydrogel microparticle degassed mold lithography Medicine R |
spellingShingle |
circulating tumor cell cell capture hydrogel microparticle degassed mold lithography Medicine R Nak Jun Lee Sejung Maeng Hyeon Ung Kim Yoon Ho Roh Changhyun Hwang Jongjin Kim Ki-Tae Hwang Ki Wan Bong Affinity-Enhanced CTC-Capturing Hydrogel Microparticles Fabricated by Degassed Mold Lithography |
topic_facet |
circulating tumor cell cell capture hydrogel microparticle degassed mold lithography Medicine R |
description |
Technologies for the detection and isolation of circulating tumor cells (CTCs) are essential in liquid biopsy, a minimally invasive technique for early diagnosis and medical intervention in cancer patients. A promising method for CTC capture, using an affinity-based approach, is the use of functionalized hydrogel microparticles (MP), which have the advantages of water-like reactivity, biologically compatible materials, and synergy with various analysis platforms. In this paper, we demonstrate the feasibility of CTC capture by hydrogel particles synthesized using a novel method called degassed mold lithography (DML). This technique increases the porosity and functionality of the MPs for effective conjugation with antibodies. Qualitative fluorescence analysis demonstrates that DML produces superior uniformity, integrity, and functionality of the MPs, as compared to conventional stop flow lithography (SFL). Analysis of the fluorescence intensity from porosity-controlled MPs by each reaction step of antibody conjugation elucidates that more antibodies are loaded when the particles are more porous. The feasibility of selective cell capture is demonstrated using breast cancer cell lines. In conclusion, using DML for the synthesis of porous MPs offers a powerful method for improving the cell affinity of the antibody-conjugated MPs. |
format |
Article in Journal/Newspaper |
author |
Nak Jun Lee Sejung Maeng Hyeon Ung Kim Yoon Ho Roh Changhyun Hwang Jongjin Kim Ki-Tae Hwang Ki Wan Bong |
author_facet |
Nak Jun Lee Sejung Maeng Hyeon Ung Kim Yoon Ho Roh Changhyun Hwang Jongjin Kim Ki-Tae Hwang Ki Wan Bong |
author_sort |
Nak Jun Lee |
title |
Affinity-Enhanced CTC-Capturing Hydrogel Microparticles Fabricated by Degassed Mold Lithography |
title_short |
Affinity-Enhanced CTC-Capturing Hydrogel Microparticles Fabricated by Degassed Mold Lithography |
title_full |
Affinity-Enhanced CTC-Capturing Hydrogel Microparticles Fabricated by Degassed Mold Lithography |
title_fullStr |
Affinity-Enhanced CTC-Capturing Hydrogel Microparticles Fabricated by Degassed Mold Lithography |
title_full_unstemmed |
Affinity-Enhanced CTC-Capturing Hydrogel Microparticles Fabricated by Degassed Mold Lithography |
title_sort |
affinity-enhanced ctc-capturing hydrogel microparticles fabricated by degassed mold lithography |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/jcm9020301 https://doaj.org/article/af29e7f3c0194f05bf88910f6c2d8f61 |
genre |
DML |
genre_facet |
DML |
op_source |
Journal of Clinical Medicine, Vol 9, Iss 2, p 301 (2020) |
op_relation |
https://www.mdpi.com/2077-0383/9/2/301 https://doaj.org/toc/2077-0383 2077-0383 doi:10.3390/jcm9020301 https://doaj.org/article/af29e7f3c0194f05bf88910f6c2d8f61 |
op_doi |
https://doi.org/10.3390/jcm9020301 |
container_title |
Journal of Clinical Medicine |
container_volume |
9 |
container_issue |
2 |
container_start_page |
301 |
_version_ |
1766397310979276800 |