Co-production of biodiesel and bioethanol using psychrophilic microalga Chlamydomonas sp. KNM0029C isolated from Arctic sea ice
Abstract Background Biofuels, generated using microalgae as sustainable energy, have received a lot of attention. Microalgae can be cultivated at low cost with CO2 and solar energy without competition from edible crops. Psychrophilic microalgae can be a suitable feedstock to produce biofuels without...
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ftdoajarticles:oai:doaj.org/article:af11ab4ee3e14885904a03cb5b2a2a69 2023-05-15T14:51:07+02:00 Co-production of biodiesel and bioethanol using psychrophilic microalga Chlamydomonas sp. KNM0029C isolated from Arctic sea ice Eun Jae Kim Sanghee Kim Han-Gu Choi Se Jong Han 2020-02-01T00:00:00Z https://doi.org/10.1186/s13068-020-1660-z https://doaj.org/article/af11ab4ee3e14885904a03cb5b2a2a69 EN eng BMC https://doi.org/10.1186/s13068-020-1660-z https://doaj.org/toc/1754-6834 doi:10.1186/s13068-020-1660-z 1754-6834 https://doaj.org/article/af11ab4ee3e14885904a03cb5b2a2a69 Biotechnology for Biofuels, Vol 13, Iss 1, Pp 1-13 (2020) Arctic Biodiesel Bioethanol Chlamydomonas Psychrophilic microalgae Fuel TP315-360 Biotechnology TP248.13-248.65 article 2020 ftdoajarticles https://doi.org/10.1186/s13068-020-1660-z 2022-12-31T02:52:45Z Abstract Background Biofuels, generated using microalgae as sustainable energy, have received a lot of attention. Microalgae can be cultivated at low cost with CO2 and solar energy without competition from edible crops. Psychrophilic microalgae can be a suitable feedstock to produce biofuels without the environmental constraints of low temperatures, because they can grow below 10 °C. However, there is a lack of efficient strategies using psychrophilic microalgae to produce biodiesel and bioethanol. Therefore, the current study aimed to optimize the production of biodiesel and bioethanol from Arctic Chlamydomonas sp. KNM0029C at low temperatures. Results After incubation in a 20-L photobioreactor, fatty acid methyl ester (FAME) was extracted using modified FAME extraction methods, producing a maximum yield of 0.16-g FAME/g KNM0029C. Residual biomass was pretreated for bioethanol production, and the yields from different methods were compared. The highest bioethanol yield (0.22-g/g residual biomass) was obtained by pretreatment with enzyme (amyloglucosidase) after sonication. Approximately 300-mg biofuel was obtained, including 156-mg FAME biodiesel and 144-mg bioethanol per g dried cells, representing the highest recorded yield from psychrophilic microalgae. Conclusions This is the first to attempt at utilizing biomass from psychrophilic Arctic microalga Chlamydomonas sp. KNM0029C for the co-production of bioethanol and biodiesel, and it yielded the highest values among reported studies using psychrophilic organisms. These results can be used as a source for the efficient biofuel production using polar microalgae. Article in Journal/Newspaper Arctic Sea ice Directory of Open Access Journals: DOAJ Articles Arctic Biotechnology for Biofuels 13 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic Biodiesel Bioethanol Chlamydomonas Psychrophilic microalgae Fuel TP315-360 Biotechnology TP248.13-248.65 |
spellingShingle |
Arctic Biodiesel Bioethanol Chlamydomonas Psychrophilic microalgae Fuel TP315-360 Biotechnology TP248.13-248.65 Eun Jae Kim Sanghee Kim Han-Gu Choi Se Jong Han Co-production of biodiesel and bioethanol using psychrophilic microalga Chlamydomonas sp. KNM0029C isolated from Arctic sea ice |
topic_facet |
Arctic Biodiesel Bioethanol Chlamydomonas Psychrophilic microalgae Fuel TP315-360 Biotechnology TP248.13-248.65 |
description |
Abstract Background Biofuels, generated using microalgae as sustainable energy, have received a lot of attention. Microalgae can be cultivated at low cost with CO2 and solar energy without competition from edible crops. Psychrophilic microalgae can be a suitable feedstock to produce biofuels without the environmental constraints of low temperatures, because they can grow below 10 °C. However, there is a lack of efficient strategies using psychrophilic microalgae to produce biodiesel and bioethanol. Therefore, the current study aimed to optimize the production of biodiesel and bioethanol from Arctic Chlamydomonas sp. KNM0029C at low temperatures. Results After incubation in a 20-L photobioreactor, fatty acid methyl ester (FAME) was extracted using modified FAME extraction methods, producing a maximum yield of 0.16-g FAME/g KNM0029C. Residual biomass was pretreated for bioethanol production, and the yields from different methods were compared. The highest bioethanol yield (0.22-g/g residual biomass) was obtained by pretreatment with enzyme (amyloglucosidase) after sonication. Approximately 300-mg biofuel was obtained, including 156-mg FAME biodiesel and 144-mg bioethanol per g dried cells, representing the highest recorded yield from psychrophilic microalgae. Conclusions This is the first to attempt at utilizing biomass from psychrophilic Arctic microalga Chlamydomonas sp. KNM0029C for the co-production of bioethanol and biodiesel, and it yielded the highest values among reported studies using psychrophilic organisms. These results can be used as a source for the efficient biofuel production using polar microalgae. |
format |
Article in Journal/Newspaper |
author |
Eun Jae Kim Sanghee Kim Han-Gu Choi Se Jong Han |
author_facet |
Eun Jae Kim Sanghee Kim Han-Gu Choi Se Jong Han |
author_sort |
Eun Jae Kim |
title |
Co-production of biodiesel and bioethanol using psychrophilic microalga Chlamydomonas sp. KNM0029C isolated from Arctic sea ice |
title_short |
Co-production of biodiesel and bioethanol using psychrophilic microalga Chlamydomonas sp. KNM0029C isolated from Arctic sea ice |
title_full |
Co-production of biodiesel and bioethanol using psychrophilic microalga Chlamydomonas sp. KNM0029C isolated from Arctic sea ice |
title_fullStr |
Co-production of biodiesel and bioethanol using psychrophilic microalga Chlamydomonas sp. KNM0029C isolated from Arctic sea ice |
title_full_unstemmed |
Co-production of biodiesel and bioethanol using psychrophilic microalga Chlamydomonas sp. KNM0029C isolated from Arctic sea ice |
title_sort |
co-production of biodiesel and bioethanol using psychrophilic microalga chlamydomonas sp. knm0029c isolated from arctic sea ice |
publisher |
BMC |
publishDate |
2020 |
url |
https://doi.org/10.1186/s13068-020-1660-z https://doaj.org/article/af11ab4ee3e14885904a03cb5b2a2a69 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Sea ice |
genre_facet |
Arctic Sea ice |
op_source |
Biotechnology for Biofuels, Vol 13, Iss 1, Pp 1-13 (2020) |
op_relation |
https://doi.org/10.1186/s13068-020-1660-z https://doaj.org/toc/1754-6834 doi:10.1186/s13068-020-1660-z 1754-6834 https://doaj.org/article/af11ab4ee3e14885904a03cb5b2a2a69 |
op_doi |
https://doi.org/10.1186/s13068-020-1660-z |
container_title |
Biotechnology for Biofuels |
container_volume |
13 |
container_issue |
1 |
_version_ |
1766322177622147072 |