A single multilocus sequence typing (MLST) scheme for seven pathogenic Leptospira species.
The available Leptospira multilocus sequence typing (MLST) scheme supported by a MLST website is limited to L. interrogans and L. kirschneri. Our aim was to broaden the utility of this scheme to incorporate a total of seven pathogenic species.We modified the existing scheme by replacing one of the s...
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ftdoajarticles:oai:doaj.org/article:aeff3f0dd5f04311845459450c7a2bcb 2023-05-15T15:12:46+02:00 A single multilocus sequence typing (MLST) scheme for seven pathogenic Leptospira species. Siriphan Boonsilp Janjira Thaipadungpanit Premjit Amornchai Vanaporn Wuthiekanun Mark S Bailey Matthew T G Holden Cuicai Zhang Xiugao Jiang Nobuo Koizumi Kyle Taylor Renee Galloway Alex R Hoffmaster Scott Craig Lee D Smythe Rudy A Hartskeerl Nicholas P Day Narisara Chantratita Edward J Feil David M Aanensen Brian G Spratt Sharon J Peacock 2013-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001954 https://doaj.org/article/aeff3f0dd5f04311845459450c7a2bcb EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3554523?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001954 https://doaj.org/article/aeff3f0dd5f04311845459450c7a2bcb PLoS Neglected Tropical Diseases, Vol 7, Iss 1, p e1954 (2013) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2013 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001954 2022-12-31T01:48:39Z The available Leptospira multilocus sequence typing (MLST) scheme supported by a MLST website is limited to L. interrogans and L. kirschneri. Our aim was to broaden the utility of this scheme to incorporate a total of seven pathogenic species.We modified the existing scheme by replacing one of the seven MLST loci (fadD was changed to caiB), as the former gene did not appear to be present in some pathogenic species. Comparison of the original and modified schemes using data for L. interrogans and L. kirschneri demonstrated that the discriminatory power of the two schemes was not significantly different. The modified scheme was used to further characterize 325 isolates (L. alexanderi [n = 5], L. borgpetersenii [n = 34], L. interrogans [n = 222], L. kirschneri [n = 29], L. noguchii [n = 9], L. santarosai [n = 10], and L. weilii [n = 16]). Phylogenetic analysis using concatenated sequences of the 7 loci demonstrated that each species corresponded to a discrete clade, and that no strains were misclassified at the species level. Comparison between genotype and serovar was possible for 254 isolates. Of the 31 sequence types (STs) represented by at least two isolates, 18 STs included isolates assigned to two or three different serovars. Conversely, 14 serovars were identified that contained between 2 to 10 different STs. New observations were made on the global phylogeography of Leptospira spp., and the utility of MLST in making associations between human disease and specific maintenance hosts was demonstrated.The new MLST scheme, supported by an updated MLST website, allows the characterization and species assignment of isolates of the seven major pathogenic species associated with leptospirosis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 7 1 e1954 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Siriphan Boonsilp Janjira Thaipadungpanit Premjit Amornchai Vanaporn Wuthiekanun Mark S Bailey Matthew T G Holden Cuicai Zhang Xiugao Jiang Nobuo Koizumi Kyle Taylor Renee Galloway Alex R Hoffmaster Scott Craig Lee D Smythe Rudy A Hartskeerl Nicholas P Day Narisara Chantratita Edward J Feil David M Aanensen Brian G Spratt Sharon J Peacock A single multilocus sequence typing (MLST) scheme for seven pathogenic Leptospira species. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The available Leptospira multilocus sequence typing (MLST) scheme supported by a MLST website is limited to L. interrogans and L. kirschneri. Our aim was to broaden the utility of this scheme to incorporate a total of seven pathogenic species.We modified the existing scheme by replacing one of the seven MLST loci (fadD was changed to caiB), as the former gene did not appear to be present in some pathogenic species. Comparison of the original and modified schemes using data for L. interrogans and L. kirschneri demonstrated that the discriminatory power of the two schemes was not significantly different. The modified scheme was used to further characterize 325 isolates (L. alexanderi [n = 5], L. borgpetersenii [n = 34], L. interrogans [n = 222], L. kirschneri [n = 29], L. noguchii [n = 9], L. santarosai [n = 10], and L. weilii [n = 16]). Phylogenetic analysis using concatenated sequences of the 7 loci demonstrated that each species corresponded to a discrete clade, and that no strains were misclassified at the species level. Comparison between genotype and serovar was possible for 254 isolates. Of the 31 sequence types (STs) represented by at least two isolates, 18 STs included isolates assigned to two or three different serovars. Conversely, 14 serovars were identified that contained between 2 to 10 different STs. New observations were made on the global phylogeography of Leptospira spp., and the utility of MLST in making associations between human disease and specific maintenance hosts was demonstrated.The new MLST scheme, supported by an updated MLST website, allows the characterization and species assignment of isolates of the seven major pathogenic species associated with leptospirosis. |
format |
Article in Journal/Newspaper |
author |
Siriphan Boonsilp Janjira Thaipadungpanit Premjit Amornchai Vanaporn Wuthiekanun Mark S Bailey Matthew T G Holden Cuicai Zhang Xiugao Jiang Nobuo Koizumi Kyle Taylor Renee Galloway Alex R Hoffmaster Scott Craig Lee D Smythe Rudy A Hartskeerl Nicholas P Day Narisara Chantratita Edward J Feil David M Aanensen Brian G Spratt Sharon J Peacock |
author_facet |
Siriphan Boonsilp Janjira Thaipadungpanit Premjit Amornchai Vanaporn Wuthiekanun Mark S Bailey Matthew T G Holden Cuicai Zhang Xiugao Jiang Nobuo Koizumi Kyle Taylor Renee Galloway Alex R Hoffmaster Scott Craig Lee D Smythe Rudy A Hartskeerl Nicholas P Day Narisara Chantratita Edward J Feil David M Aanensen Brian G Spratt Sharon J Peacock |
author_sort |
Siriphan Boonsilp |
title |
A single multilocus sequence typing (MLST) scheme for seven pathogenic Leptospira species. |
title_short |
A single multilocus sequence typing (MLST) scheme for seven pathogenic Leptospira species. |
title_full |
A single multilocus sequence typing (MLST) scheme for seven pathogenic Leptospira species. |
title_fullStr |
A single multilocus sequence typing (MLST) scheme for seven pathogenic Leptospira species. |
title_full_unstemmed |
A single multilocus sequence typing (MLST) scheme for seven pathogenic Leptospira species. |
title_sort |
single multilocus sequence typing (mlst) scheme for seven pathogenic leptospira species. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pntd.0001954 https://doaj.org/article/aeff3f0dd5f04311845459450c7a2bcb |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 7, Iss 1, p e1954 (2013) |
op_relation |
http://europepmc.org/articles/PMC3554523?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001954 https://doaj.org/article/aeff3f0dd5f04311845459450c7a2bcb |
op_doi |
https://doi.org/10.1371/journal.pntd.0001954 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
7 |
container_issue |
1 |
container_start_page |
e1954 |
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1766343408604938240 |