Geographic variation of individual venom profile of Crotalus durissus snakes
Abstract Background: South American rattlesnakes are represented in Brazil by a single species, Crotalus durissus, which has public health importance due to the severity of its envenomation and to its wide geographical distribution. The species is subdivided into several subspecies, but the current...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:aefc5961241446dd8da06fd461b590f2 2023-05-15T15:16:52+02:00 Geographic variation of individual venom profile of Crotalus durissus snakes Leandro Norberto da Silva-Júnior Lara de Souza Abreu Caroline Fabri Bittencourt Rodrigues Nathália da Costa Galizio Weslei da Silva Aguiar Caroline Serino-Silva Valdomiro Souza dos Santos Isabella Alves Costa Luis Vicente Franco Oliveira Sávio Stefanini Sant’Anna Kathleen Fernandes Grego Anita Mitico Tanaka-Azevedo Leandro Nascimento da Silva Rodrigues Karen de Morais-Zani 2020-08-01T00:00:00Z https://doi.org/10.1590/1678-9199-jvatitd-2020-0016 https://doaj.org/article/aefc5961241446dd8da06fd461b590f2 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100325&tlng=en http://www.scielo.br/pdf/jvatitd/v26/1678-9199-jvatitd-26-e20200016.pdf https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2020-0016 https://doaj.org/article/aefc5961241446dd8da06fd461b590f2 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 26 (2020) Intraspecific venom variability Geographic venom variability Crotalus durissus collilineatus Crotalus durissus terrificus Snake venom composition and function Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2020 ftdoajarticles https://doi.org/10.1590/1678-9199-jvatitd-2020-0016 2022-12-31T15:15:39Z Abstract Background: South American rattlesnakes are represented in Brazil by a single species, Crotalus durissus, which has public health importance due to the severity of its envenomation and to its wide geographical distribution. The species is subdivided into several subspecies, but the current classification is controversial. In Brazil, the venoms of C. d. terrificus and C. d. collilineatus are used for hyperimmunization of horses for antivenom production, even though the distinction of these two subspecies are mostly by their geographical distribution. In this context, we described a comparative compositional and functional characterization of individual C. d. collilineatus and C. d. terrificus venoms from three Brazilian states. Methods: We compared the compositional patterns of C. d. terrificus and C. d. collilineatus individual venoms by 1-DE and RP-HPLC. For functional analyzes, the enzymatic activities of PLA2, LAAO, and coagulant activity were evaluated. Finally, the immunorecognition of venom toxins by the crotalic antivenom produced at Butantan Institute was evaluated using Western blotting. Results: The protein profile of individual venoms from C. d. collilineatus and C. d. terrificus showed a comparable overall composition, despite some intraspecific variation, especially regarding crotamine and LAAO. Interestingly, HPLC analysis showed a geographic pattern concerning PLA2. In addition, a remarkable intraspecific variation was also observed in PLA2, LAAO and coagulant activities. The immunorecognition pattern of individual venoms from C. d. collilineatus and C. d. terrificus by crotalic antivenom produced at Butantan Institute was similar. Conclusions: The results highlighted the individual variability among the venoms of C. durissus ssp. specimens. Importantly, our data point to a geographical variation of C. durissus ssp. venom profile, regardless of the subspecies, as evidenced by PLA2 isoforms complexity, which may explain the increase in venom neurotoxicity from Northeastern through Southern ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 26 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Intraspecific venom variability Geographic venom variability Crotalus durissus collilineatus Crotalus durissus terrificus Snake venom composition and function Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Intraspecific venom variability Geographic venom variability Crotalus durissus collilineatus Crotalus durissus terrificus Snake venom composition and function Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Leandro Norberto da Silva-Júnior Lara de Souza Abreu Caroline Fabri Bittencourt Rodrigues Nathália da Costa Galizio Weslei da Silva Aguiar Caroline Serino-Silva Valdomiro Souza dos Santos Isabella Alves Costa Luis Vicente Franco Oliveira Sávio Stefanini Sant’Anna Kathleen Fernandes Grego Anita Mitico Tanaka-Azevedo Leandro Nascimento da Silva Rodrigues Karen de Morais-Zani Geographic variation of individual venom profile of Crotalus durissus snakes |
topic_facet |
Intraspecific venom variability Geographic venom variability Crotalus durissus collilineatus Crotalus durissus terrificus Snake venom composition and function Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Abstract Background: South American rattlesnakes are represented in Brazil by a single species, Crotalus durissus, which has public health importance due to the severity of its envenomation and to its wide geographical distribution. The species is subdivided into several subspecies, but the current classification is controversial. In Brazil, the venoms of C. d. terrificus and C. d. collilineatus are used for hyperimmunization of horses for antivenom production, even though the distinction of these two subspecies are mostly by their geographical distribution. In this context, we described a comparative compositional and functional characterization of individual C. d. collilineatus and C. d. terrificus venoms from three Brazilian states. Methods: We compared the compositional patterns of C. d. terrificus and C. d. collilineatus individual venoms by 1-DE and RP-HPLC. For functional analyzes, the enzymatic activities of PLA2, LAAO, and coagulant activity were evaluated. Finally, the immunorecognition of venom toxins by the crotalic antivenom produced at Butantan Institute was evaluated using Western blotting. Results: The protein profile of individual venoms from C. d. collilineatus and C. d. terrificus showed a comparable overall composition, despite some intraspecific variation, especially regarding crotamine and LAAO. Interestingly, HPLC analysis showed a geographic pattern concerning PLA2. In addition, a remarkable intraspecific variation was also observed in PLA2, LAAO and coagulant activities. The immunorecognition pattern of individual venoms from C. d. collilineatus and C. d. terrificus by crotalic antivenom produced at Butantan Institute was similar. Conclusions: The results highlighted the individual variability among the venoms of C. durissus ssp. specimens. Importantly, our data point to a geographical variation of C. durissus ssp. venom profile, regardless of the subspecies, as evidenced by PLA2 isoforms complexity, which may explain the increase in venom neurotoxicity from Northeastern through Southern ... |
format |
Article in Journal/Newspaper |
author |
Leandro Norberto da Silva-Júnior Lara de Souza Abreu Caroline Fabri Bittencourt Rodrigues Nathália da Costa Galizio Weslei da Silva Aguiar Caroline Serino-Silva Valdomiro Souza dos Santos Isabella Alves Costa Luis Vicente Franco Oliveira Sávio Stefanini Sant’Anna Kathleen Fernandes Grego Anita Mitico Tanaka-Azevedo Leandro Nascimento da Silva Rodrigues Karen de Morais-Zani |
author_facet |
Leandro Norberto da Silva-Júnior Lara de Souza Abreu Caroline Fabri Bittencourt Rodrigues Nathália da Costa Galizio Weslei da Silva Aguiar Caroline Serino-Silva Valdomiro Souza dos Santos Isabella Alves Costa Luis Vicente Franco Oliveira Sávio Stefanini Sant’Anna Kathleen Fernandes Grego Anita Mitico Tanaka-Azevedo Leandro Nascimento da Silva Rodrigues Karen de Morais-Zani |
author_sort |
Leandro Norberto da Silva-Júnior |
title |
Geographic variation of individual venom profile of Crotalus durissus snakes |
title_short |
Geographic variation of individual venom profile of Crotalus durissus snakes |
title_full |
Geographic variation of individual venom profile of Crotalus durissus snakes |
title_fullStr |
Geographic variation of individual venom profile of Crotalus durissus snakes |
title_full_unstemmed |
Geographic variation of individual venom profile of Crotalus durissus snakes |
title_sort |
geographic variation of individual venom profile of crotalus durissus snakes |
publisher |
SciELO |
publishDate |
2020 |
url |
https://doi.org/10.1590/1678-9199-jvatitd-2020-0016 https://doaj.org/article/aefc5961241446dd8da06fd461b590f2 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 26 (2020) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100325&tlng=en http://www.scielo.br/pdf/jvatitd/v26/1678-9199-jvatitd-26-e20200016.pdf https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2020-0016 https://doaj.org/article/aefc5961241446dd8da06fd461b590f2 |
op_doi |
https://doi.org/10.1590/1678-9199-jvatitd-2020-0016 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
26 |
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1766347170988949504 |