Effects of temperature and fertilization on the structure of total versus active bacterial communities from sub-Antarctic seawater exposed to crude oil and diesel fuel
Polar environments are exposed to the risk of oil pollution. However, there is limited knowledge regarding how the variation of physicochemical factors influencing biodegradation may affect bacterial community structure. The effects of temperature (4, 10 and 20°C) and organic fertilizatio...
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Norwegian Polar Institute
2013
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Online Access: | https://doi.org/10.3402/polar.v32i0.18521 https://doaj.org/article/aebdcc3006f84d7e872ac99215525c21 |
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ftdoajarticles:oai:doaj.org/article:aebdcc3006f84d7e872ac99215525c21 2023-05-15T13:43:59+02:00 Effects of temperature and fertilization on the structure of total versus active bacterial communities from sub-Antarctic seawater exposed to crude oil and diesel fuel Arturo Rodríguez-Blanco Audrey Duval Emilien Pelletier Daniel Delille Jean-François Ghiglione 2013-05-01T00:00:00Z https://doi.org/10.3402/polar.v32i0.18521 https://doaj.org/article/aebdcc3006f84d7e872ac99215525c21 EN eng Norwegian Polar Institute http://www.polarresearch.net/index.php/polar/article/download/18521/pdf_1 https://doaj.org/toc/0800-0395 https://doaj.org/toc/1751-8369 doi:10.3402/polar.v32i0.18521 0800-0395 1751-8369 https://doaj.org/article/aebdcc3006f84d7e872ac99215525c21 Polar Research, Vol 32, Iss 0, Pp 1-11 (2013) Oil hydrocarbons Inipol EAP 22 temperature 16S rDNA/rRNA sub-Antarctic seawater Environmental sciences GE1-350 Oceanography GC1-1581 article 2013 ftdoajarticles https://doi.org/10.3402/polar.v32i0.18521 2022-12-30T21:42:53Z Polar environments are exposed to the risk of oil pollution. However, there is limited knowledge regarding how the variation of physicochemical factors influencing biodegradation may affect bacterial community structure. The effects of temperature (4, 10 and 20°C) and organic fertilization (Inipol EAP 22) on community structure and diversity of bacteria inhabiting Kerguelen sub-Antarctic waters were studied in crude- and diesel-amended microcosms. Dynamics of total (i.e., 16S rDNA-based) and metabolically active (i.e., 16S rRNA-based) bacterial community structure and diversity were monitored using capillary-electrophoresis single-strand conformation polymorphism. Results showed that total and active community structures were differently influenced by temperature and fertilization in the presence of hydrocarbons. Both fertilization and temperature induced changes in total community structure in the presence of crude oil and diesel. However, temperature showed a limited influence on active community structure, and fertilization induced changes in the presence of crude oil only. Simpson's index decreased for total bacterial communities at all temperatures in the presence of crude oil and diesel, whereas a lower reduction was observed for active bacterial populations. In the presence of fertilizer, the diversity of the whole community approached control values after seven incubation weeks; this was not observed for the active bacterial community. This study evidenced qualitative differences in total and active bacterial community structures of Kerguelen seawaters in the presence of hydrocarbons and different responses relative to variation in temperature and fertilization. These factors and hydrocarbons composition have to be taken into account to understand bacterial community dynamics after an oil spill. Article in Journal/Newspaper Antarc* Antarctic Polar Research Directory of Open Access Journals: DOAJ Articles Antarctic Kerguelen Polar Research 32 1 18521 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Oil hydrocarbons Inipol EAP 22 temperature 16S rDNA/rRNA sub-Antarctic seawater Environmental sciences GE1-350 Oceanography GC1-1581 |
spellingShingle |
Oil hydrocarbons Inipol EAP 22 temperature 16S rDNA/rRNA sub-Antarctic seawater Environmental sciences GE1-350 Oceanography GC1-1581 Arturo Rodríguez-Blanco Audrey Duval Emilien Pelletier Daniel Delille Jean-François Ghiglione Effects of temperature and fertilization on the structure of total versus active bacterial communities from sub-Antarctic seawater exposed to crude oil and diesel fuel |
topic_facet |
Oil hydrocarbons Inipol EAP 22 temperature 16S rDNA/rRNA sub-Antarctic seawater Environmental sciences GE1-350 Oceanography GC1-1581 |
description |
Polar environments are exposed to the risk of oil pollution. However, there is limited knowledge regarding how the variation of physicochemical factors influencing biodegradation may affect bacterial community structure. The effects of temperature (4, 10 and 20°C) and organic fertilization (Inipol EAP 22) on community structure and diversity of bacteria inhabiting Kerguelen sub-Antarctic waters were studied in crude- and diesel-amended microcosms. Dynamics of total (i.e., 16S rDNA-based) and metabolically active (i.e., 16S rRNA-based) bacterial community structure and diversity were monitored using capillary-electrophoresis single-strand conformation polymorphism. Results showed that total and active community structures were differently influenced by temperature and fertilization in the presence of hydrocarbons. Both fertilization and temperature induced changes in total community structure in the presence of crude oil and diesel. However, temperature showed a limited influence on active community structure, and fertilization induced changes in the presence of crude oil only. Simpson's index decreased for total bacterial communities at all temperatures in the presence of crude oil and diesel, whereas a lower reduction was observed for active bacterial populations. In the presence of fertilizer, the diversity of the whole community approached control values after seven incubation weeks; this was not observed for the active bacterial community. This study evidenced qualitative differences in total and active bacterial community structures of Kerguelen seawaters in the presence of hydrocarbons and different responses relative to variation in temperature and fertilization. These factors and hydrocarbons composition have to be taken into account to understand bacterial community dynamics after an oil spill. |
format |
Article in Journal/Newspaper |
author |
Arturo Rodríguez-Blanco Audrey Duval Emilien Pelletier Daniel Delille Jean-François Ghiglione |
author_facet |
Arturo Rodríguez-Blanco Audrey Duval Emilien Pelletier Daniel Delille Jean-François Ghiglione |
author_sort |
Arturo Rodríguez-Blanco |
title |
Effects of temperature and fertilization on the structure of total versus active bacterial communities from sub-Antarctic seawater exposed to crude oil and diesel fuel |
title_short |
Effects of temperature and fertilization on the structure of total versus active bacterial communities from sub-Antarctic seawater exposed to crude oil and diesel fuel |
title_full |
Effects of temperature and fertilization on the structure of total versus active bacterial communities from sub-Antarctic seawater exposed to crude oil and diesel fuel |
title_fullStr |
Effects of temperature and fertilization on the structure of total versus active bacterial communities from sub-Antarctic seawater exposed to crude oil and diesel fuel |
title_full_unstemmed |
Effects of temperature and fertilization on the structure of total versus active bacterial communities from sub-Antarctic seawater exposed to crude oil and diesel fuel |
title_sort |
effects of temperature and fertilization on the structure of total versus active bacterial communities from sub-antarctic seawater exposed to crude oil and diesel fuel |
publisher |
Norwegian Polar Institute |
publishDate |
2013 |
url |
https://doi.org/10.3402/polar.v32i0.18521 https://doaj.org/article/aebdcc3006f84d7e872ac99215525c21 |
geographic |
Antarctic Kerguelen |
geographic_facet |
Antarctic Kerguelen |
genre |
Antarc* Antarctic Polar Research |
genre_facet |
Antarc* Antarctic Polar Research |
op_source |
Polar Research, Vol 32, Iss 0, Pp 1-11 (2013) |
op_relation |
http://www.polarresearch.net/index.php/polar/article/download/18521/pdf_1 https://doaj.org/toc/0800-0395 https://doaj.org/toc/1751-8369 doi:10.3402/polar.v32i0.18521 0800-0395 1751-8369 https://doaj.org/article/aebdcc3006f84d7e872ac99215525c21 |
op_doi |
https://doi.org/10.3402/polar.v32i0.18521 |
container_title |
Polar Research |
container_volume |
32 |
container_issue |
1 |
container_start_page |
18521 |
_version_ |
1766195615209881600 |