MEMBRANE FRACTIONS FROM Strongyloides venezuelensis IN THE IMMUNODIAGNOSIS OF HUMAN STRONGYLOIDIASIS

• Published in: Revista do Instituto de Medicina Tropical de São Paulo
• Main Authors: Marcelo Andreetta Corral, Fabiana Martins Paula, Maiara Gottardi, Dirce Mary Correia Lima Meisel, Pedro Paulo Chieffi, Ronaldo César Borges Gryschek
• Format: Article in Journal/Newspaper
• Language: English
• Published: Universidade de São Paulo (USP) 2015
• Subjects: Strongyloides venezuelensis; Parasitological diagnosis; Immunological diagnosis; Membrane fractions; Arctic medicine. Tropical medicine; RC955-962; Infectious and parasitic diseases; RC109-216; Arctic
• Online Access: https://doi.org/10.1590/S0036-46652015000100011; https://doaj.org/article/ae7dcfbdfeb842b0895d52da0f4123c2

Strongyloides venezuelensis is a parasitic nematode of rodents frequently used to obtain heterologous antigens for the immunological diagnosis of human strongyloidiasis. The aim of this study was to evaluate membrane fractions from S. venezuelensis for human strongyloidiasis immunodiagnosis. Soluble and membrane fractions were obtained in phosphate saline (SS and SM) and Tris-HCl (TS and TM) from filariform larvae of S. venezuelensis. Ninety-two serum samples (n = 92) were obtained from 20 strongyloidiasis patients (Group I), 32 from patients with other parasitic diseases (Group II), and 40 from healthy individuals (Group III), and were analyzed by enzyme-linked immunosorbent assay (ELISA). Soluble fractions (SS and TS) showed 90.0% sensitivity and 88.9% specificity, whereas the membrane fractions (SM and TM) showed 95.0% sensitivity and 94.4% specificity. The present results suggest the possible use of membrane fractions of S. venezuelensis as an alternative antigen for human strongyloidiasis immunodiagnosis.