Evolution of duplicated IgH loci in Atlantic salmon, Salmo salar
Abstract Background The Atlantic salmon ( Salmo salar ) immunoglobulin heavy chain ( IgH ) locus possesses two parallel IgH isoloci ( IGH-A and IGH-B ), that are related to the genomic duplication event in the family Salmonidae. These duplicated IgH loci in Atlantic salmon provide a unique opportuni...
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ftdoajarticles:oai:doaj.org/article:ae79220c1c874b0794d9bd4bc3360a17 2023-05-15T15:28:43+02:00 Evolution of duplicated IgH loci in Atlantic salmon, Salmo salar Yasuike Motoshige de Boer Johan von Schalburg Kristian R Cooper Glenn A McKinnel Linda Messmer Amber So Stacy Davidson William S Koop Ben F 2010-09-01T00:00:00Z https://doi.org/10.1186/1471-2164-11-486 https://doaj.org/article/ae79220c1c874b0794d9bd4bc3360a17 EN eng BMC http://www.biomedcentral.com/1471-2164/11/486 https://doaj.org/toc/1471-2164 doi:10.1186/1471-2164-11-486 1471-2164 https://doaj.org/article/ae79220c1c874b0794d9bd4bc3360a17 BMC Genomics, Vol 11, Iss 1, p 486 (2010) Biotechnology TP248.13-248.65 Genetics QH426-470 article 2010 ftdoajarticles https://doi.org/10.1186/1471-2164-11-486 2022-12-31T08:32:54Z Abstract Background The Atlantic salmon ( Salmo salar ) immunoglobulin heavy chain ( IgH ) locus possesses two parallel IgH isoloci ( IGH-A and IGH-B ), that are related to the genomic duplication event in the family Salmonidae. These duplicated IgH loci in Atlantic salmon provide a unique opportunity to examine the mechanisms of genome diversity and genome evolution of the IgH loci in vertebrates. In this study, we defined the structure of these loci in Atlantic salmon, and sequenced 24 bacterial artificial chromosome (BAC) clones that were assembled into the IGH-A (1.1 Mb) and IGH-B (0.9 Mb) loci. In addition, over 7,000 cDNA clones from the IgH variable (V<smcaps>H</smcaps>) region have been sequenced and analyzed. Results The present study shows that the genomic organization of the duplicated IgH loci in Atlantic salmon differs from that in other teleosts and other vertebrates. The loci possess multiple Cτ genes upstream of the Cμ region, with three of the Cτ genes being functional. Moreover, the duplicated loci possess over 300 V<smcaps>H</smcaps> segments which could be classified into 18 families. This is the largest number of V<smcaps>H</smcaps> families currently defined in any vertebrate. There were significant structural differences between the two loci, indicating that both IGH-A and -B loci have evolved independently in the short time after the recent genome duplication approximately 60 mya. Conclusions Our results indicate that the duplication of the IgH loci in Atlantic salmon significantly contributes to the increased diversity of the antibody repertoire, as compared with the single IgH locus in other vertebrates. Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles BMC Genomics 11 1 486 |
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Directory of Open Access Journals: DOAJ Articles |
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Biotechnology TP248.13-248.65 Genetics QH426-470 |
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Biotechnology TP248.13-248.65 Genetics QH426-470 Yasuike Motoshige de Boer Johan von Schalburg Kristian R Cooper Glenn A McKinnel Linda Messmer Amber So Stacy Davidson William S Koop Ben F Evolution of duplicated IgH loci in Atlantic salmon, Salmo salar |
topic_facet |
Biotechnology TP248.13-248.65 Genetics QH426-470 |
description |
Abstract Background The Atlantic salmon ( Salmo salar ) immunoglobulin heavy chain ( IgH ) locus possesses two parallel IgH isoloci ( IGH-A and IGH-B ), that are related to the genomic duplication event in the family Salmonidae. These duplicated IgH loci in Atlantic salmon provide a unique opportunity to examine the mechanisms of genome diversity and genome evolution of the IgH loci in vertebrates. In this study, we defined the structure of these loci in Atlantic salmon, and sequenced 24 bacterial artificial chromosome (BAC) clones that were assembled into the IGH-A (1.1 Mb) and IGH-B (0.9 Mb) loci. In addition, over 7,000 cDNA clones from the IgH variable (V<smcaps>H</smcaps>) region have been sequenced and analyzed. Results The present study shows that the genomic organization of the duplicated IgH loci in Atlantic salmon differs from that in other teleosts and other vertebrates. The loci possess multiple Cτ genes upstream of the Cμ region, with three of the Cτ genes being functional. Moreover, the duplicated loci possess over 300 V<smcaps>H</smcaps> segments which could be classified into 18 families. This is the largest number of V<smcaps>H</smcaps> families currently defined in any vertebrate. There were significant structural differences between the two loci, indicating that both IGH-A and -B loci have evolved independently in the short time after the recent genome duplication approximately 60 mya. Conclusions Our results indicate that the duplication of the IgH loci in Atlantic salmon significantly contributes to the increased diversity of the antibody repertoire, as compared with the single IgH locus in other vertebrates. |
format |
Article in Journal/Newspaper |
author |
Yasuike Motoshige de Boer Johan von Schalburg Kristian R Cooper Glenn A McKinnel Linda Messmer Amber So Stacy Davidson William S Koop Ben F |
author_facet |
Yasuike Motoshige de Boer Johan von Schalburg Kristian R Cooper Glenn A McKinnel Linda Messmer Amber So Stacy Davidson William S Koop Ben F |
author_sort |
Yasuike Motoshige |
title |
Evolution of duplicated IgH loci in Atlantic salmon, Salmo salar |
title_short |
Evolution of duplicated IgH loci in Atlantic salmon, Salmo salar |
title_full |
Evolution of duplicated IgH loci in Atlantic salmon, Salmo salar |
title_fullStr |
Evolution of duplicated IgH loci in Atlantic salmon, Salmo salar |
title_full_unstemmed |
Evolution of duplicated IgH loci in Atlantic salmon, Salmo salar |
title_sort |
evolution of duplicated igh loci in atlantic salmon, salmo salar |
publisher |
BMC |
publishDate |
2010 |
url |
https://doi.org/10.1186/1471-2164-11-486 https://doaj.org/article/ae79220c1c874b0794d9bd4bc3360a17 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
BMC Genomics, Vol 11, Iss 1, p 486 (2010) |
op_relation |
http://www.biomedcentral.com/1471-2164/11/486 https://doaj.org/toc/1471-2164 doi:10.1186/1471-2164-11-486 1471-2164 https://doaj.org/article/ae79220c1c874b0794d9bd4bc3360a17 |
op_doi |
https://doi.org/10.1186/1471-2164-11-486 |
container_title |
BMC Genomics |
container_volume |
11 |
container_issue |
1 |
container_start_page |
486 |
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1766359051949572096 |