Paclitaxel-loaded biodegradable ROS-sensitive nanoparticles for cancer therapy
Abhilash D Pandya,1 Eliézer Jäger,2 Shahla Bagheri Fam,3 Anita Höcherl,2 Alessandro Jäger,2 Vladimir Sincari,2 Bo Nyström,4 Petr Štěpánek,2 Tore Skotland,5 Kirsten Sandvig,5,6 Martin Hrubý,2 Gunhild M Mælandsmo1,71Department of Tumor Biology, Institute for Cancer Research, Oslo University Hospital,...
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ftdoajarticles:oai:doaj.org/article:ae5a20d5062441a4968f7b39df48feca 2023-05-15T18:49:27+02:00 Paclitaxel-loaded biodegradable ROS-sensitive nanoparticles for cancer therapy Pandya AD Jäger E Bagheri Fam S Höcherl A Jäger A Sincari V Nyström B Štěpánek P Skotland T Sandvig K Hrubý M Mælandsmo GM 2019-08-01T00:00:00Z https://doaj.org/article/ae5a20d5062441a4968f7b39df48feca EN eng Dove Medical Press https://www.dovepress.com/paclitaxel-loaded-biodegradable-ros-sensitive-nanoparticles-for-cancer-peer-reviewed-article-IJN https://doaj.org/toc/1178-2013 1178-2013 https://doaj.org/article/ae5a20d5062441a4968f7b39df48feca International Journal of Nanomedicine, Vol Volume 14, Pp 6269-6285 (2019) Reactive oxygen species ROS-sensitive nanoparticles Paclitaxel Treatment efficacy Macrophage infiltration Medicine (General) R5-920 article 2019 ftdoajarticles 2022-12-31T08:01:38Z Abhilash D Pandya,1 Eliézer Jäger,2 Shahla Bagheri Fam,3 Anita Höcherl,2 Alessandro Jäger,2 Vladimir Sincari,2 Bo Nyström,4 Petr Štěpánek,2 Tore Skotland,5 Kirsten Sandvig,5,6 Martin Hrubý,2 Gunhild M Mælandsmo1,71Department of Tumor Biology, Institute for Cancer Research, Oslo University Hospital, The Norwegian Radium Hospital, Oslo, Norway; 2Institute of Macromolecular Chemistry v.v.i, Academy of Sciences of the Czech Republic, Prague, Czech Republic; 3Department of Radiation Biology, Institute for Cancer Research, Oslo University Hospital, The Norwegian Radium Hospital, Oslo, Norway; 4Department of Chemistry, University of Oslo, Oslo, Norway; 5Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital, The Norwegian Radium Hospital, Oslo, Norway; 6Department of Biosciences, University of Oslo, Oslo, Norway; 7Institute of Medical Biology, Faculty of Health Sciences, The Arctic University of Norway – University of Tromsø, Tromsø, NorwayBackground: Reactive oxygen species (ROS), such as hydrogen peroxide and superoxide, trigger biodegradation of polymer-based nanoparticles (NPs) bearing pinacol-type boronic ester groups. These NPs may selectively release their cargo, in this case paclitaxel (PTX), at the high levels of ROS present in the intracellular environment of inflamed tissues and most tumors.Purpose: The main objective was to determine anti-tumor efficacy of PTX-loaded ROS-sensitive NPs and to examine whether macrophage infiltration had any impact on treatment efficacy.Methods: NPs were synthesized and their characteristics in the presence of H2O2 were demonstrated. Both confocal microscopy as well as flow cytometry approaches were used to determine degradation of ROS-sensitive NPs. HeLa cells were cultured in vitro and used to establish tumor xenografts in nude mice. In vivo experiments were performed to understand toxicity, biodistribution and anti-tumor efficacy of the NPs. Moreover, we performed immunohistochemistry on tumor sections to study infiltration of M1 and ... Article in Journal/Newspaper Arctic University of Norway University of Tromsø Directory of Open Access Journals: DOAJ Articles Arctic Norway Tromsø |
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ftdoajarticles |
language |
English |
topic |
Reactive oxygen species ROS-sensitive nanoparticles Paclitaxel Treatment efficacy Macrophage infiltration Medicine (General) R5-920 |
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Reactive oxygen species ROS-sensitive nanoparticles Paclitaxel Treatment efficacy Macrophage infiltration Medicine (General) R5-920 Pandya AD Jäger E Bagheri Fam S Höcherl A Jäger A Sincari V Nyström B Štěpánek P Skotland T Sandvig K Hrubý M Mælandsmo GM Paclitaxel-loaded biodegradable ROS-sensitive nanoparticles for cancer therapy |
topic_facet |
Reactive oxygen species ROS-sensitive nanoparticles Paclitaxel Treatment efficacy Macrophage infiltration Medicine (General) R5-920 |
description |
Abhilash D Pandya,1 Eliézer Jäger,2 Shahla Bagheri Fam,3 Anita Höcherl,2 Alessandro Jäger,2 Vladimir Sincari,2 Bo Nyström,4 Petr Štěpánek,2 Tore Skotland,5 Kirsten Sandvig,5,6 Martin Hrubý,2 Gunhild M Mælandsmo1,71Department of Tumor Biology, Institute for Cancer Research, Oslo University Hospital, The Norwegian Radium Hospital, Oslo, Norway; 2Institute of Macromolecular Chemistry v.v.i, Academy of Sciences of the Czech Republic, Prague, Czech Republic; 3Department of Radiation Biology, Institute for Cancer Research, Oslo University Hospital, The Norwegian Radium Hospital, Oslo, Norway; 4Department of Chemistry, University of Oslo, Oslo, Norway; 5Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital, The Norwegian Radium Hospital, Oslo, Norway; 6Department of Biosciences, University of Oslo, Oslo, Norway; 7Institute of Medical Biology, Faculty of Health Sciences, The Arctic University of Norway – University of Tromsø, Tromsø, NorwayBackground: Reactive oxygen species (ROS), such as hydrogen peroxide and superoxide, trigger biodegradation of polymer-based nanoparticles (NPs) bearing pinacol-type boronic ester groups. These NPs may selectively release their cargo, in this case paclitaxel (PTX), at the high levels of ROS present in the intracellular environment of inflamed tissues and most tumors.Purpose: The main objective was to determine anti-tumor efficacy of PTX-loaded ROS-sensitive NPs and to examine whether macrophage infiltration had any impact on treatment efficacy.Methods: NPs were synthesized and their characteristics in the presence of H2O2 were demonstrated. Both confocal microscopy as well as flow cytometry approaches were used to determine degradation of ROS-sensitive NPs. HeLa cells were cultured in vitro and used to establish tumor xenografts in nude mice. In vivo experiments were performed to understand toxicity, biodistribution and anti-tumor efficacy of the NPs. Moreover, we performed immunohistochemistry on tumor sections to study infiltration of M1 and ... |
format |
Article in Journal/Newspaper |
author |
Pandya AD Jäger E Bagheri Fam S Höcherl A Jäger A Sincari V Nyström B Štěpánek P Skotland T Sandvig K Hrubý M Mælandsmo GM |
author_facet |
Pandya AD Jäger E Bagheri Fam S Höcherl A Jäger A Sincari V Nyström B Štěpánek P Skotland T Sandvig K Hrubý M Mælandsmo GM |
author_sort |
Pandya AD |
title |
Paclitaxel-loaded biodegradable ROS-sensitive nanoparticles for cancer therapy |
title_short |
Paclitaxel-loaded biodegradable ROS-sensitive nanoparticles for cancer therapy |
title_full |
Paclitaxel-loaded biodegradable ROS-sensitive nanoparticles for cancer therapy |
title_fullStr |
Paclitaxel-loaded biodegradable ROS-sensitive nanoparticles for cancer therapy |
title_full_unstemmed |
Paclitaxel-loaded biodegradable ROS-sensitive nanoparticles for cancer therapy |
title_sort |
paclitaxel-loaded biodegradable ros-sensitive nanoparticles for cancer therapy |
publisher |
Dove Medical Press |
publishDate |
2019 |
url |
https://doaj.org/article/ae5a20d5062441a4968f7b39df48feca |
geographic |
Arctic Norway Tromsø |
geographic_facet |
Arctic Norway Tromsø |
genre |
Arctic University of Norway University of Tromsø |
genre_facet |
Arctic University of Norway University of Tromsø |
op_source |
International Journal of Nanomedicine, Vol Volume 14, Pp 6269-6285 (2019) |
op_relation |
https://www.dovepress.com/paclitaxel-loaded-biodegradable-ros-sensitive-nanoparticles-for-cancer-peer-reviewed-article-IJN https://doaj.org/toc/1178-2013 1178-2013 https://doaj.org/article/ae5a20d5062441a4968f7b39df48feca |
_version_ |
1766243040816529408 |