Unearthing soil arthropod diversity through DNA metabarcoding
DNA metabarcoding has the potential to greatly advance understanding of soil biodiversity, but this approach has seen limited application for the most abundant and species-rich group of soil fauna–the arthropods. This study begins to address this gap by comparing information on species composition r...
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ftdoajarticles:oai:doaj.org/article:ae336c2b8a7740caae334d46800ed682 2024-01-07T09:41:28+01:00 Unearthing soil arthropod diversity through DNA metabarcoding Monica R. Young Paul D. N. Hebert 2022-02-01T00:00:00Z https://doi.org/10.7717/peerj.12845 https://doaj.org/article/ae336c2b8a7740caae334d46800ed682 EN eng PeerJ Inc. https://peerj.com/articles/12845.pdf https://peerj.com/articles/12845/ https://doaj.org/toc/2167-8359 doi:10.7717/peerj.12845 2167-8359 https://doaj.org/article/ae336c2b8a7740caae334d46800ed682 PeerJ, Vol 10, p e12845 (2022) Metabarcoding Arthropods Soil Biodiversity NGS Environmental DNA Medicine R Biology (General) QH301-705.5 article 2022 ftdoajarticles https://doi.org/10.7717/peerj.12845 2023-12-10T01:52:30Z DNA metabarcoding has the potential to greatly advance understanding of soil biodiversity, but this approach has seen limited application for the most abundant and species-rich group of soil fauna–the arthropods. This study begins to address this gap by comparing information on species composition recovered from metabarcoding two types of bulk samples (specimens, soil) from a temperate zone site and from bulk soil samples collected at eight sites in the Arctic. Analysis of 22 samples (3 specimen, 19 soil) revealed 410 arthropod OTUs belonging to 112 families, 25 orders, and nine classes. Studies at the temperate zone site revealed little overlap in species composition between soil and specimen samples, but more overlap at higher taxonomic levels (families, orders) and congruent patterns of α- and β-diversity. Expansion of soil analyses to the Arctic revealed locally rich, highly dissimilar, and spatially structured assemblages compatible with dispersal limited and environmentally driven assembly. The current study demonstrates that DNA metabarcoding of bulk soil enables rapid, large-scale assessments of soil arthropod diversity. However, deep sequence coverage is required to adequately capture the species present in these samples, and expansion of the DNA barcode reference library is necessary to improve taxonomic resolution of the sequences recovered through this approach. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PeerJ 10 e12845 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Metabarcoding Arthropods Soil Biodiversity NGS Environmental DNA Medicine R Biology (General) QH301-705.5 |
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Metabarcoding Arthropods Soil Biodiversity NGS Environmental DNA Medicine R Biology (General) QH301-705.5 Monica R. Young Paul D. N. Hebert Unearthing soil arthropod diversity through DNA metabarcoding |
topic_facet |
Metabarcoding Arthropods Soil Biodiversity NGS Environmental DNA Medicine R Biology (General) QH301-705.5 |
description |
DNA metabarcoding has the potential to greatly advance understanding of soil biodiversity, but this approach has seen limited application for the most abundant and species-rich group of soil fauna–the arthropods. This study begins to address this gap by comparing information on species composition recovered from metabarcoding two types of bulk samples (specimens, soil) from a temperate zone site and from bulk soil samples collected at eight sites in the Arctic. Analysis of 22 samples (3 specimen, 19 soil) revealed 410 arthropod OTUs belonging to 112 families, 25 orders, and nine classes. Studies at the temperate zone site revealed little overlap in species composition between soil and specimen samples, but more overlap at higher taxonomic levels (families, orders) and congruent patterns of α- and β-diversity. Expansion of soil analyses to the Arctic revealed locally rich, highly dissimilar, and spatially structured assemblages compatible with dispersal limited and environmentally driven assembly. The current study demonstrates that DNA metabarcoding of bulk soil enables rapid, large-scale assessments of soil arthropod diversity. However, deep sequence coverage is required to adequately capture the species present in these samples, and expansion of the DNA barcode reference library is necessary to improve taxonomic resolution of the sequences recovered through this approach. |
format |
Article in Journal/Newspaper |
author |
Monica R. Young Paul D. N. Hebert |
author_facet |
Monica R. Young Paul D. N. Hebert |
author_sort |
Monica R. Young |
title |
Unearthing soil arthropod diversity through DNA metabarcoding |
title_short |
Unearthing soil arthropod diversity through DNA metabarcoding |
title_full |
Unearthing soil arthropod diversity through DNA metabarcoding |
title_fullStr |
Unearthing soil arthropod diversity through DNA metabarcoding |
title_full_unstemmed |
Unearthing soil arthropod diversity through DNA metabarcoding |
title_sort |
unearthing soil arthropod diversity through dna metabarcoding |
publisher |
PeerJ Inc. |
publishDate |
2022 |
url |
https://doi.org/10.7717/peerj.12845 https://doaj.org/article/ae336c2b8a7740caae334d46800ed682 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PeerJ, Vol 10, p e12845 (2022) |
op_relation |
https://peerj.com/articles/12845.pdf https://peerj.com/articles/12845/ https://doaj.org/toc/2167-8359 doi:10.7717/peerj.12845 2167-8359 https://doaj.org/article/ae336c2b8a7740caae334d46800ed682 |
op_doi |
https://doi.org/10.7717/peerj.12845 |
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PeerJ |
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10 |
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e12845 |
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