Anti-inflammatory properties of oolong tea (Camellia sinensis) ethanol extract and epigallocatechin gallate in LPS-induced RAW 264.7 cells
Objective: To evaluate the anti-inflammatory activity of oolong tea ethanol extract (OTEE) and epigallocatechin gallate (EGCG) on lipopolysaccharide-induced murine macrophage cell line (RAW 264.7). Methods: A cytotoxic assay using MTS tetrazolium was conducted to find a nontoxic level of OTEE and EG...
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Wolters Kluwer Medknow Publications
2017
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Online Access: | https://doi.org/10.1016/j.apjtb.2017.10.002 https://doaj.org/article/ae172e1923cd41c7a87e37a5e937c713 |
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ftdoajarticles:oai:doaj.org/article:ae172e1923cd41c7a87e37a5e937c713 2023-05-15T15:11:31+02:00 Anti-inflammatory properties of oolong tea (Camellia sinensis) ethanol extract and epigallocatechin gallate in LPS-induced RAW 264.7 cells Arina Novilla Dedi Somantri Djamhuri Betty Nurhayati Dwi Davidson Rihibiha Ervi Afifah Wahyu Widowati 2017-11-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2017.10.002 https://doaj.org/article/ae172e1923cd41c7a87e37a5e937c713 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169117310687 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2017.10.002 https://doaj.org/article/ae172e1923cd41c7a87e37a5e937c713 Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 11, Pp 1005-1009 (2017) Inflammation Camellia sinensis EGCG Macrophages Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2017 ftdoajarticles https://doi.org/10.1016/j.apjtb.2017.10.002 2022-12-30T22:58:16Z Objective: To evaluate the anti-inflammatory activity of oolong tea ethanol extract (OTEE) and epigallocatechin gallate (EGCG) on lipopolysaccharide-induced murine macrophage cell line (RAW 264.7). Methods: A cytotoxic assay using MTS tetrazolium was conducted to find a nontoxic level of OTEE and EGCG toward RAW 264.7 cells. Interleukins (IL-6, IL-1β), tumor necrosis factor-α (TNF-α), and cyclooxigenase-2 (COX-2) levels were measured by ELISA, and nitric oxide (NO) levels measured by a nitrate/nitrite colorimetric assay to determine the inhibition activity of OTEE and EGCG. Results: Lipopolysaccharide induction increases NO, COX-2, IL-6, IL-1β, and TNF-α levels compared with the untreated cell (negative control). The positive control, lipopolysaccharide-induced RAW 264.7 without treatments showed the highest level of all pro-inflammatory cytokines and modulators tested in this study. The positive control was used as standard to obtain OTEE and EGCG inhibition activity toward NO, COX-2, IL-6, IL-1β, and TNF-α. OTEE had a higher inhibition activity toward NO, COX-2, IL-6, and IL-1β than EGCG; the reverse was seen for TNF-α. However, both OTEE and EGCG suppressed production of NO, COX-2, IL-6, IL-1β, and TNF-α. Conclusions: OTEE and EGCG have the potential for use as anti-inflammatory drugs, which is shown by their ability to reduce the production of NO, COX-2, IL-6, IL-1β, and TNF-α in active macrophages. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 7 11 1005 1009 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Inflammation Camellia sinensis EGCG Macrophages Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Inflammation Camellia sinensis EGCG Macrophages Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Arina Novilla Dedi Somantri Djamhuri Betty Nurhayati Dwi Davidson Rihibiha Ervi Afifah Wahyu Widowati Anti-inflammatory properties of oolong tea (Camellia sinensis) ethanol extract and epigallocatechin gallate in LPS-induced RAW 264.7 cells |
topic_facet |
Inflammation Camellia sinensis EGCG Macrophages Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To evaluate the anti-inflammatory activity of oolong tea ethanol extract (OTEE) and epigallocatechin gallate (EGCG) on lipopolysaccharide-induced murine macrophage cell line (RAW 264.7). Methods: A cytotoxic assay using MTS tetrazolium was conducted to find a nontoxic level of OTEE and EGCG toward RAW 264.7 cells. Interleukins (IL-6, IL-1β), tumor necrosis factor-α (TNF-α), and cyclooxigenase-2 (COX-2) levels were measured by ELISA, and nitric oxide (NO) levels measured by a nitrate/nitrite colorimetric assay to determine the inhibition activity of OTEE and EGCG. Results: Lipopolysaccharide induction increases NO, COX-2, IL-6, IL-1β, and TNF-α levels compared with the untreated cell (negative control). The positive control, lipopolysaccharide-induced RAW 264.7 without treatments showed the highest level of all pro-inflammatory cytokines and modulators tested in this study. The positive control was used as standard to obtain OTEE and EGCG inhibition activity toward NO, COX-2, IL-6, IL-1β, and TNF-α. OTEE had a higher inhibition activity toward NO, COX-2, IL-6, and IL-1β than EGCG; the reverse was seen for TNF-α. However, both OTEE and EGCG suppressed production of NO, COX-2, IL-6, IL-1β, and TNF-α. Conclusions: OTEE and EGCG have the potential for use as anti-inflammatory drugs, which is shown by their ability to reduce the production of NO, COX-2, IL-6, IL-1β, and TNF-α in active macrophages. |
format |
Article in Journal/Newspaper |
author |
Arina Novilla Dedi Somantri Djamhuri Betty Nurhayati Dwi Davidson Rihibiha Ervi Afifah Wahyu Widowati |
author_facet |
Arina Novilla Dedi Somantri Djamhuri Betty Nurhayati Dwi Davidson Rihibiha Ervi Afifah Wahyu Widowati |
author_sort |
Arina Novilla |
title |
Anti-inflammatory properties of oolong tea (Camellia sinensis) ethanol extract and epigallocatechin gallate in LPS-induced RAW 264.7 cells |
title_short |
Anti-inflammatory properties of oolong tea (Camellia sinensis) ethanol extract and epigallocatechin gallate in LPS-induced RAW 264.7 cells |
title_full |
Anti-inflammatory properties of oolong tea (Camellia sinensis) ethanol extract and epigallocatechin gallate in LPS-induced RAW 264.7 cells |
title_fullStr |
Anti-inflammatory properties of oolong tea (Camellia sinensis) ethanol extract and epigallocatechin gallate in LPS-induced RAW 264.7 cells |
title_full_unstemmed |
Anti-inflammatory properties of oolong tea (Camellia sinensis) ethanol extract and epigallocatechin gallate in LPS-induced RAW 264.7 cells |
title_sort |
anti-inflammatory properties of oolong tea (camellia sinensis) ethanol extract and epigallocatechin gallate in lps-induced raw 264.7 cells |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2017 |
url |
https://doi.org/10.1016/j.apjtb.2017.10.002 https://doaj.org/article/ae172e1923cd41c7a87e37a5e937c713 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 11, Pp 1005-1009 (2017) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169117310687 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2017.10.002 https://doaj.org/article/ae172e1923cd41c7a87e37a5e937c713 |
op_doi |
https://doi.org/10.1016/j.apjtb.2017.10.002 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
7 |
container_issue |
11 |
container_start_page |
1005 |
op_container_end_page |
1009 |
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1766342364782133248 |