Antibacterial Properties of Fucoidans from the Brown Algae Fucus vesiculosus L. of the Barents Sea
Fucoidans, sulfated polysaccharides found in cell walls of brown algae, are considered as a promising antimicrobial component for various applications in medicine and the food industry. In this study, we compare the antibacterial properties of two fractions of fucoidan from the brown algae Fucus ves...
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MDPI AG
2021
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Online Access: | https://doi.org/10.3390/biology10010067 https://doaj.org/article/addd276538b94055b13076c4ce281393 |
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ftdoajarticles:oai:doaj.org/article:addd276538b94055b13076c4ce281393 2024-01-07T09:42:24+01:00 Antibacterial Properties of Fucoidans from the Brown Algae Fucus vesiculosus L. of the Barents Sea Olga N. Ayrapetyan Ekaterina D. Obluchinskaya Elena V. Zhurishkina Yury A. Skorik Dmitry V. Lebedev Anna A. Kulminskaya Irina M. Lapina 2021-01-01T00:00:00Z https://doi.org/10.3390/biology10010067 https://doaj.org/article/addd276538b94055b13076c4ce281393 EN eng MDPI AG https://www.mdpi.com/2079-7737/10/1/67 https://doaj.org/toc/2079-7737 doi:10.3390/biology10010067 2079-7737 https://doaj.org/article/addd276538b94055b13076c4ce281393 Biology, Vol 10, Iss 1, p 67 (2021) Fucus vesiculosus fucoidan antibacterial activity atomic force microscopy Biology (General) QH301-705.5 article 2021 ftdoajarticles https://doi.org/10.3390/biology10010067 2023-12-10T01:44:50Z Fucoidans, sulfated polysaccharides found in cell walls of brown algae, are considered as a promising antimicrobial component for various applications in medicine and the food industry. In this study, we compare the antibacterial properties of two fractions of fucoidan from the brown algae Fucus vesiculosus gathered in the littoral of the Barents Sea and sampled at different stages of purification. The crude fraction of fucoidan was isolated from algae by extraction with aqueous ethanol and sonication. The purified fraction was obtained by additional treatment of the crude fraction with a solution of calcium chloride. The structural features of both fractions were characterized in detail and their antibacterial effects against several Gram-positive and Gram-negative bacteria were compared by photometry, acridine orange staining assay, and atomic force microscopy. Fucoidan inhibited growth in all of the above microorganisms, showing a bacteriostatic effect with minimum inhibitory concentrations (MIC) in the range between 4 and 6 mg/mL, with E. coli being the most sensitive to both fractions. Changes in the chemical composition after treatment of the crude fraction with a solution of calcium chloride led to a decrease in the content of sulfates and uronic acids and diminished antibacterial activity. Article in Journal/Newspaper Barents Sea Directory of Open Access Journals: DOAJ Articles Barents Sea Biology 10 1 67 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Fucus vesiculosus fucoidan antibacterial activity atomic force microscopy Biology (General) QH301-705.5 |
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Fucus vesiculosus fucoidan antibacterial activity atomic force microscopy Biology (General) QH301-705.5 Olga N. Ayrapetyan Ekaterina D. Obluchinskaya Elena V. Zhurishkina Yury A. Skorik Dmitry V. Lebedev Anna A. Kulminskaya Irina M. Lapina Antibacterial Properties of Fucoidans from the Brown Algae Fucus vesiculosus L. of the Barents Sea |
topic_facet |
Fucus vesiculosus fucoidan antibacterial activity atomic force microscopy Biology (General) QH301-705.5 |
description |
Fucoidans, sulfated polysaccharides found in cell walls of brown algae, are considered as a promising antimicrobial component for various applications in medicine and the food industry. In this study, we compare the antibacterial properties of two fractions of fucoidan from the brown algae Fucus vesiculosus gathered in the littoral of the Barents Sea and sampled at different stages of purification. The crude fraction of fucoidan was isolated from algae by extraction with aqueous ethanol and sonication. The purified fraction was obtained by additional treatment of the crude fraction with a solution of calcium chloride. The structural features of both fractions were characterized in detail and their antibacterial effects against several Gram-positive and Gram-negative bacteria were compared by photometry, acridine orange staining assay, and atomic force microscopy. Fucoidan inhibited growth in all of the above microorganisms, showing a bacteriostatic effect with minimum inhibitory concentrations (MIC) in the range between 4 and 6 mg/mL, with E. coli being the most sensitive to both fractions. Changes in the chemical composition after treatment of the crude fraction with a solution of calcium chloride led to a decrease in the content of sulfates and uronic acids and diminished antibacterial activity. |
format |
Article in Journal/Newspaper |
author |
Olga N. Ayrapetyan Ekaterina D. Obluchinskaya Elena V. Zhurishkina Yury A. Skorik Dmitry V. Lebedev Anna A. Kulminskaya Irina M. Lapina |
author_facet |
Olga N. Ayrapetyan Ekaterina D. Obluchinskaya Elena V. Zhurishkina Yury A. Skorik Dmitry V. Lebedev Anna A. Kulminskaya Irina M. Lapina |
author_sort |
Olga N. Ayrapetyan |
title |
Antibacterial Properties of Fucoidans from the Brown Algae Fucus vesiculosus L. of the Barents Sea |
title_short |
Antibacterial Properties of Fucoidans from the Brown Algae Fucus vesiculosus L. of the Barents Sea |
title_full |
Antibacterial Properties of Fucoidans from the Brown Algae Fucus vesiculosus L. of the Barents Sea |
title_fullStr |
Antibacterial Properties of Fucoidans from the Brown Algae Fucus vesiculosus L. of the Barents Sea |
title_full_unstemmed |
Antibacterial Properties of Fucoidans from the Brown Algae Fucus vesiculosus L. of the Barents Sea |
title_sort |
antibacterial properties of fucoidans from the brown algae fucus vesiculosus l. of the barents sea |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/biology10010067 https://doaj.org/article/addd276538b94055b13076c4ce281393 |
geographic |
Barents Sea |
geographic_facet |
Barents Sea |
genre |
Barents Sea |
genre_facet |
Barents Sea |
op_source |
Biology, Vol 10, Iss 1, p 67 (2021) |
op_relation |
https://www.mdpi.com/2079-7737/10/1/67 https://doaj.org/toc/2079-7737 doi:10.3390/biology10010067 2079-7737 https://doaj.org/article/addd276538b94055b13076c4ce281393 |
op_doi |
https://doi.org/10.3390/biology10010067 |
container_title |
Biology |
container_volume |
10 |
container_issue |
1 |
container_start_page |
67 |
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1787423367422279680 |