Formulation and Biomedical Activity of Oil-in-Water Nanoemulsion Combining Tinospora smilacina Water Extract and Calophyllum inophyllum Seeds Oil
Elnaz Saki,1 Vinuthaa Murthy,1 Hao Wang,1 Roshanak Khandanlou,1 Johanna Wapling,2 Richard Weir3 1Faculty of Science and Technology, Charles Darwin University, Darwin, Northern Territory, Australia; 2Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia;...
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ftdoajarticles:oai:doaj.org/article:ada6c96dbae343df94af1ca63481a0bc 2023-06-11T04:11:45+02:00 Formulation and Biomedical Activity of Oil-in-Water Nanoemulsion Combining Tinospora smilacina Water Extract and Calophyllum inophyllum Seeds Oil Saki E Murthy V Wang H Khandanlou R Wapling J Weir R 2023-05-01T00:00:00Z https://doaj.org/article/ada6c96dbae343df94af1ca63481a0bc EN eng Dove Medical Press https://www.dovepress.com/formulation-and-biomedical-activity-of-oil-in-water-nanoemulsion-combi-peer-reviewed-fulltext-article-CCID https://doaj.org/toc/1178-7015 1178-7015 https://doaj.org/article/ada6c96dbae343df94af1ca63481a0bc Clinical, Cosmetic and Investigational Dermatology, Vol Volume 16, Pp 1159-1174 (2023) medicinal plants nanoemulsion rsm wound healing invitro Dermatology RL1-803 article 2023 ftdoajarticles 2023-05-07T00:34:08Z Elnaz Saki,1 Vinuthaa Murthy,1 Hao Wang,1 Roshanak Khandanlou,1 Johanna Wapling,2 Richard Weir3 1Faculty of Science and Technology, Charles Darwin University, Darwin, Northern Territory, Australia; 2Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia; 3Department of Industry, Tourism and Trade, Berrimah Veterinary Laboratory, Darwin, Northern Territory, AustraliaCorrespondence: Elnaz Saki, Faculty of Science and Technology, Charles Darwin University, Darwin, Northern Territory, 0909, Australia, Tel +61 42439 3238, Email elnaz.saki@cdu.edu.auIntroduction: Tinospora smilacina is a native plant used in traditional medicine by First Nations peoples in Australia to treat inflammation. In our previous study, an optimised Calophyllum inophyllum seed oil (CSO) nanoemulsion (NE) showed improved biomedical activities such as antimicrobial, antioxidant activity, cell viability and in vitro wound healing efficacy compared to CSO.Methods: In this study, a stable NE formulation combining T. smilacina water extract (TSWE) and CSO in a nanoemulsion (CTNE) was prepared to integrate the bioactive compounds in both native plants and improve wound healing efficacy. D-optimal mixture design was used to optimise the physicochemical characteristics of the CTNE, including droplet size and polydispersity index (PDI). Cell viability and in vitro wound healing studies were done in the presence of CTNE, TSWE and CSO against a clone of baby hamster kidney fibroblasts (BHK-21 cell clone BSR-T7/5).Results: The optimised CTNE had a 24 ± 5 nm particle size and 0.21± 0.02 PDI value and was stable after four weeks each at 4 °C and room temperature. According to the results, incorporating TSWE into CTNE improved its antioxidant activity, cell viability, and ability to promote wound healing. The study also revealed that TSWE has > 6% higher antioxidant activity than CSO. While CTNE did not significantly impact mammalian cell viability, it exhibited wound-healing properties in the BSR cell line ... Article in Journal/Newspaper First Nations Directory of Open Access Journals: DOAJ Articles |
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Directory of Open Access Journals: DOAJ Articles |
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medicinal plants nanoemulsion rsm wound healing invitro Dermatology RL1-803 |
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medicinal plants nanoemulsion rsm wound healing invitro Dermatology RL1-803 Saki E Murthy V Wang H Khandanlou R Wapling J Weir R Formulation and Biomedical Activity of Oil-in-Water Nanoemulsion Combining Tinospora smilacina Water Extract and Calophyllum inophyllum Seeds Oil |
topic_facet |
medicinal plants nanoemulsion rsm wound healing invitro Dermatology RL1-803 |
description |
Elnaz Saki,1 Vinuthaa Murthy,1 Hao Wang,1 Roshanak Khandanlou,1 Johanna Wapling,2 Richard Weir3 1Faculty of Science and Technology, Charles Darwin University, Darwin, Northern Territory, Australia; 2Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia; 3Department of Industry, Tourism and Trade, Berrimah Veterinary Laboratory, Darwin, Northern Territory, AustraliaCorrespondence: Elnaz Saki, Faculty of Science and Technology, Charles Darwin University, Darwin, Northern Territory, 0909, Australia, Tel +61 42439 3238, Email elnaz.saki@cdu.edu.auIntroduction: Tinospora smilacina is a native plant used in traditional medicine by First Nations peoples in Australia to treat inflammation. In our previous study, an optimised Calophyllum inophyllum seed oil (CSO) nanoemulsion (NE) showed improved biomedical activities such as antimicrobial, antioxidant activity, cell viability and in vitro wound healing efficacy compared to CSO.Methods: In this study, a stable NE formulation combining T. smilacina water extract (TSWE) and CSO in a nanoemulsion (CTNE) was prepared to integrate the bioactive compounds in both native plants and improve wound healing efficacy. D-optimal mixture design was used to optimise the physicochemical characteristics of the CTNE, including droplet size and polydispersity index (PDI). Cell viability and in vitro wound healing studies were done in the presence of CTNE, TSWE and CSO against a clone of baby hamster kidney fibroblasts (BHK-21 cell clone BSR-T7/5).Results: The optimised CTNE had a 24 ± 5 nm particle size and 0.21± 0.02 PDI value and was stable after four weeks each at 4 °C and room temperature. According to the results, incorporating TSWE into CTNE improved its antioxidant activity, cell viability, and ability to promote wound healing. The study also revealed that TSWE has > 6% higher antioxidant activity than CSO. While CTNE did not significantly impact mammalian cell viability, it exhibited wound-healing properties in the BSR cell line ... |
format |
Article in Journal/Newspaper |
author |
Saki E Murthy V Wang H Khandanlou R Wapling J Weir R |
author_facet |
Saki E Murthy V Wang H Khandanlou R Wapling J Weir R |
author_sort |
Saki E |
title |
Formulation and Biomedical Activity of Oil-in-Water Nanoemulsion Combining Tinospora smilacina Water Extract and Calophyllum inophyllum Seeds Oil |
title_short |
Formulation and Biomedical Activity of Oil-in-Water Nanoemulsion Combining Tinospora smilacina Water Extract and Calophyllum inophyllum Seeds Oil |
title_full |
Formulation and Biomedical Activity of Oil-in-Water Nanoemulsion Combining Tinospora smilacina Water Extract and Calophyllum inophyllum Seeds Oil |
title_fullStr |
Formulation and Biomedical Activity of Oil-in-Water Nanoemulsion Combining Tinospora smilacina Water Extract and Calophyllum inophyllum Seeds Oil |
title_full_unstemmed |
Formulation and Biomedical Activity of Oil-in-Water Nanoemulsion Combining Tinospora smilacina Water Extract and Calophyllum inophyllum Seeds Oil |
title_sort |
formulation and biomedical activity of oil-in-water nanoemulsion combining tinospora smilacina water extract and calophyllum inophyllum seeds oil |
publisher |
Dove Medical Press |
publishDate |
2023 |
url |
https://doaj.org/article/ada6c96dbae343df94af1ca63481a0bc |
genre |
First Nations |
genre_facet |
First Nations |
op_source |
Clinical, Cosmetic and Investigational Dermatology, Vol Volume 16, Pp 1159-1174 (2023) |
op_relation |
https://www.dovepress.com/formulation-and-biomedical-activity-of-oil-in-water-nanoemulsion-combi-peer-reviewed-fulltext-article-CCID https://doaj.org/toc/1178-7015 1178-7015 https://doaj.org/article/ada6c96dbae343df94af1ca63481a0bc |
_version_ |
1768387036891316224 |