Phytochemical and in vitro biological investigations of methanolic extracts of Enhydra fluctuans Lour.
Objective: To study the phytochemical and biological properties (antioxidant, anthelmintic and thrombolytic) of methanolic extracts of Enhydra fluctuans Lour., a plant belonging to the Asteraceae family. Methods: The phytochemical evaluation was carried out by qualitative analysis. In vitro antioxid...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wolters Kluwer Medknow Publications
2014
|
Subjects: | |
Online Access: | https://doi.org/10.12980/APJTB.4.2014C677 https://doaj.org/article/ad9cee22abeb4dc68db88fabcf57456a |
id |
ftdoajarticles:oai:doaj.org/article:ad9cee22abeb4dc68db88fabcf57456a |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:ad9cee22abeb4dc68db88fabcf57456a 2023-05-15T15:11:09+02:00 Phytochemical and in vitro biological investigations of methanolic extracts of Enhydra fluctuans Lour. Sourov Kuri Md. Mustahsan Billah S.M. Masud Rana Zannatul Naim Md. Mahmodul Islam Md. Hasanuzzaman Md. Ramjan Ali Rana Banik 2014-04-01T00:00:00Z https://doi.org/10.12980/APJTB.4.2014C677 https://doaj.org/article/ad9cee22abeb4dc68db88fabcf57456a EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115302100 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.2014C677 https://doaj.org/article/ad9cee22abeb4dc68db88fabcf57456a Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss 4, Pp 299-305 (2014) Enhydra fluctuans Lour Phytochemicals Antioxidant DPPH radical scavenging Pheretima posthuma Streptokinase Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2014 ftdoajarticles https://doi.org/10.12980/APJTB.4.2014C677 2022-12-31T00:14:11Z Objective: To study the phytochemical and biological properties (antioxidant, anthelmintic and thrombolytic) of methanolic extracts of Enhydra fluctuans Lour., a plant belonging to the Asteraceae family. Methods: The phytochemical evaluation was carried out by qualitative analysis. In vitro antioxidant activity of extract was studied using free radical scavenging assay, ability of reduction, total phenol and total flavonoid contents determination assays. The anthelmintic activity was determined using paralysis and death time of Pheretima posthuma (earthworm) and thrombolytic activity by clot disruption assay. Results: The phytochemical evaluation showed significant presence of flavonoids, triterpenes, carbohydrate, reducing sugars, saponins, phenols, diterpenes, protein and tannin. The antioxidant activity was found significant [IC50=(135.20±0.56) μg/mL] as compared to ascorbic acid [(130.00±0.76) µg/mL]. The reducing power was increased with concentration. Total phenol and total flavonoid contents were (153.08±0.38) mg/mL and (172.04±0.56) mg/mL respectively. The paralysis and death time of earthworms for different concentrations of extract were determined and compared with albendazole. The results showed that 10 mg/mL of the crude extract had similar effect with albendazole. Additionally, the crude extract showed a concentration depended relationship with its anthelmintic property. The clot lysis activity of crude extract was compared to the standard streptokinase's clot lysis (40.13%) activity and found significant (31%). Conclusions: The study proves that the crude methanolic extract of Enhydra fluctuans Lour. has significant antioxidant, anthelmintic and thrombolytic activity containing wide range of phytochemicals. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 4 4 299 305 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Enhydra fluctuans Lour Phytochemicals Antioxidant DPPH radical scavenging Pheretima posthuma Streptokinase Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Enhydra fluctuans Lour Phytochemicals Antioxidant DPPH radical scavenging Pheretima posthuma Streptokinase Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Sourov Kuri Md. Mustahsan Billah S.M. Masud Rana Zannatul Naim Md. Mahmodul Islam Md. Hasanuzzaman Md. Ramjan Ali Rana Banik Phytochemical and in vitro biological investigations of methanolic extracts of Enhydra fluctuans Lour. |
topic_facet |
Enhydra fluctuans Lour Phytochemicals Antioxidant DPPH radical scavenging Pheretima posthuma Streptokinase Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To study the phytochemical and biological properties (antioxidant, anthelmintic and thrombolytic) of methanolic extracts of Enhydra fluctuans Lour., a plant belonging to the Asteraceae family. Methods: The phytochemical evaluation was carried out by qualitative analysis. In vitro antioxidant activity of extract was studied using free radical scavenging assay, ability of reduction, total phenol and total flavonoid contents determination assays. The anthelmintic activity was determined using paralysis and death time of Pheretima posthuma (earthworm) and thrombolytic activity by clot disruption assay. Results: The phytochemical evaluation showed significant presence of flavonoids, triterpenes, carbohydrate, reducing sugars, saponins, phenols, diterpenes, protein and tannin. The antioxidant activity was found significant [IC50=(135.20±0.56) μg/mL] as compared to ascorbic acid [(130.00±0.76) µg/mL]. The reducing power was increased with concentration. Total phenol and total flavonoid contents were (153.08±0.38) mg/mL and (172.04±0.56) mg/mL respectively. The paralysis and death time of earthworms for different concentrations of extract were determined and compared with albendazole. The results showed that 10 mg/mL of the crude extract had similar effect with albendazole. Additionally, the crude extract showed a concentration depended relationship with its anthelmintic property. The clot lysis activity of crude extract was compared to the standard streptokinase's clot lysis (40.13%) activity and found significant (31%). Conclusions: The study proves that the crude methanolic extract of Enhydra fluctuans Lour. has significant antioxidant, anthelmintic and thrombolytic activity containing wide range of phytochemicals. |
format |
Article in Journal/Newspaper |
author |
Sourov Kuri Md. Mustahsan Billah S.M. Masud Rana Zannatul Naim Md. Mahmodul Islam Md. Hasanuzzaman Md. Ramjan Ali Rana Banik |
author_facet |
Sourov Kuri Md. Mustahsan Billah S.M. Masud Rana Zannatul Naim Md. Mahmodul Islam Md. Hasanuzzaman Md. Ramjan Ali Rana Banik |
author_sort |
Sourov Kuri |
title |
Phytochemical and in vitro biological investigations of methanolic extracts of Enhydra fluctuans Lour. |
title_short |
Phytochemical and in vitro biological investigations of methanolic extracts of Enhydra fluctuans Lour. |
title_full |
Phytochemical and in vitro biological investigations of methanolic extracts of Enhydra fluctuans Lour. |
title_fullStr |
Phytochemical and in vitro biological investigations of methanolic extracts of Enhydra fluctuans Lour. |
title_full_unstemmed |
Phytochemical and in vitro biological investigations of methanolic extracts of Enhydra fluctuans Lour. |
title_sort |
phytochemical and in vitro biological investigations of methanolic extracts of enhydra fluctuans lour. |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2014 |
url |
https://doi.org/10.12980/APJTB.4.2014C677 https://doaj.org/article/ad9cee22abeb4dc68db88fabcf57456a |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss 4, Pp 299-305 (2014) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115302100 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.2014C677 https://doaj.org/article/ad9cee22abeb4dc68db88fabcf57456a |
op_doi |
https://doi.org/10.12980/APJTB.4.2014C677 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
4 |
container_issue |
4 |
container_start_page |
299 |
op_container_end_page |
305 |
_version_ |
1766342053273272320 |