Shotgun Lipidomics for the Determination of Phospholipid and Eicosanoid Profiles in Atlantic Salmon ( Salmo salar L.) Muscle Tissue Using Electrospray Ionization (ESI)-MS/MS Spectrometric Analysis
Shotgun lipidomics was applied to identify and quantify phospholipids (PLs) in salmon muscle tissue by focusing on the distribution of ω-3 fatty acids (e.g., docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA)) in the form of phospholipids, as well as to identify and quantify eicosanoids, whi...
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ftdoajarticles:oai:doaj.org/article:ad98c0d41ba64928a72a500d8658f0ed 2024-01-14T10:05:27+01:00 Shotgun Lipidomics for the Determination of Phospholipid and Eicosanoid Profiles in Atlantic Salmon ( Salmo salar L.) Muscle Tissue Using Electrospray Ionization (ESI)-MS/MS Spectrometric Analysis JuDong Yeo Christopher C. Parrish 2021-02-01T00:00:00Z https://doi.org/10.3390/ijms22052272 https://doaj.org/article/ad98c0d41ba64928a72a500d8658f0ed EN eng MDPI AG https://www.mdpi.com/1422-0067/22/5/2272 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms22052272 1422-0067 1661-6596 https://doaj.org/article/ad98c0d41ba64928a72a500d8658f0ed International Journal of Molecular Sciences, Vol 22, Iss 5, p 2272 (2021) eicosanoid MS/MS phospholipids salmon lipidomics Biology (General) QH301-705.5 Chemistry QD1-999 article 2021 ftdoajarticles https://doi.org/10.3390/ijms22052272 2023-12-17T01:45:09Z Shotgun lipidomics was applied to identify and quantify phospholipids (PLs) in salmon muscle tissue by focusing on the distribution of ω-3 fatty acids (e.g., docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA)) in the form of phospholipids, as well as to identify and quantify eicosanoids, which has not yet been attempted in Atlantic salmon muscle. Shotgun lipidomics enabled the identification of 43 PL species belonging to four different classes: phosphatidylcholines (PCs), phosphatidylethanolamines (PEs), phosphatidylserines (PSs), and phosphatidylinositols (PIs). Among others, 16:0-22:6 PtdCho m/z [M + Na] + at 828.4 was the predominant PL species in salmon muscle tissue. The present study provided the quantification of individual phospholipid species, which has not been performed for salmon muscle tissue so far. In addition, two eicosanoids—prostaglandin E2 (PGE2) and prostaglandin F3α (PGF3α)—were identified for the first time in salmon muscle. Thus, the rapid and high-throughput shotgun lipidomics approach should shed new light on phospholipids and eicosanoids in salmon muscle tissue. Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles International Journal of Molecular Sciences 22 5 2272 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
eicosanoid MS/MS phospholipids salmon lipidomics Biology (General) QH301-705.5 Chemistry QD1-999 |
spellingShingle |
eicosanoid MS/MS phospholipids salmon lipidomics Biology (General) QH301-705.5 Chemistry QD1-999 JuDong Yeo Christopher C. Parrish Shotgun Lipidomics for the Determination of Phospholipid and Eicosanoid Profiles in Atlantic Salmon ( Salmo salar L.) Muscle Tissue Using Electrospray Ionization (ESI)-MS/MS Spectrometric Analysis |
topic_facet |
eicosanoid MS/MS phospholipids salmon lipidomics Biology (General) QH301-705.5 Chemistry QD1-999 |
description |
Shotgun lipidomics was applied to identify and quantify phospholipids (PLs) in salmon muscle tissue by focusing on the distribution of ω-3 fatty acids (e.g., docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA)) in the form of phospholipids, as well as to identify and quantify eicosanoids, which has not yet been attempted in Atlantic salmon muscle. Shotgun lipidomics enabled the identification of 43 PL species belonging to four different classes: phosphatidylcholines (PCs), phosphatidylethanolamines (PEs), phosphatidylserines (PSs), and phosphatidylinositols (PIs). Among others, 16:0-22:6 PtdCho m/z [M + Na] + at 828.4 was the predominant PL species in salmon muscle tissue. The present study provided the quantification of individual phospholipid species, which has not been performed for salmon muscle tissue so far. In addition, two eicosanoids—prostaglandin E2 (PGE2) and prostaglandin F3α (PGF3α)—were identified for the first time in salmon muscle. Thus, the rapid and high-throughput shotgun lipidomics approach should shed new light on phospholipids and eicosanoids in salmon muscle tissue. |
format |
Article in Journal/Newspaper |
author |
JuDong Yeo Christopher C. Parrish |
author_facet |
JuDong Yeo Christopher C. Parrish |
author_sort |
JuDong Yeo |
title |
Shotgun Lipidomics for the Determination of Phospholipid and Eicosanoid Profiles in Atlantic Salmon ( Salmo salar L.) Muscle Tissue Using Electrospray Ionization (ESI)-MS/MS Spectrometric Analysis |
title_short |
Shotgun Lipidomics for the Determination of Phospholipid and Eicosanoid Profiles in Atlantic Salmon ( Salmo salar L.) Muscle Tissue Using Electrospray Ionization (ESI)-MS/MS Spectrometric Analysis |
title_full |
Shotgun Lipidomics for the Determination of Phospholipid and Eicosanoid Profiles in Atlantic Salmon ( Salmo salar L.) Muscle Tissue Using Electrospray Ionization (ESI)-MS/MS Spectrometric Analysis |
title_fullStr |
Shotgun Lipidomics for the Determination of Phospholipid and Eicosanoid Profiles in Atlantic Salmon ( Salmo salar L.) Muscle Tissue Using Electrospray Ionization (ESI)-MS/MS Spectrometric Analysis |
title_full_unstemmed |
Shotgun Lipidomics for the Determination of Phospholipid and Eicosanoid Profiles in Atlantic Salmon ( Salmo salar L.) Muscle Tissue Using Electrospray Ionization (ESI)-MS/MS Spectrometric Analysis |
title_sort |
shotgun lipidomics for the determination of phospholipid and eicosanoid profiles in atlantic salmon ( salmo salar l.) muscle tissue using electrospray ionization (esi)-ms/ms spectrometric analysis |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/ijms22052272 https://doaj.org/article/ad98c0d41ba64928a72a500d8658f0ed |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
International Journal of Molecular Sciences, Vol 22, Iss 5, p 2272 (2021) |
op_relation |
https://www.mdpi.com/1422-0067/22/5/2272 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms22052272 1422-0067 1661-6596 https://doaj.org/article/ad98c0d41ba64928a72a500d8658f0ed |
op_doi |
https://doi.org/10.3390/ijms22052272 |
container_title |
International Journal of Molecular Sciences |
container_volume |
22 |
container_issue |
5 |
container_start_page |
2272 |
_version_ |
1788059809352777728 |