Comparative Proteomics of Ostreid Herpesvirus 1 and Pacific Oyster Interactions With Two Families Exhibiting Contrasted Susceptibility to Viral Infection
Massive mortality outbreaks affecting Pacific oysters (Crassostrea gigas) spat/juveniles are often associated with the detection of a herpesvirus called ostreid herpesvirus type 1 (OsHV-1). In this work, experimental infection trials of C. gigas spat with OsHV-1 were conducted using two contrasted P...
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ftdoajarticles:oai:doaj.org/article:ad79cc3d48924a3a9b9555b70e349290 2023-05-15T15:59:04+02:00 Comparative Proteomics of Ostreid Herpesvirus 1 and Pacific Oyster Interactions With Two Families Exhibiting Contrasted Susceptibility to Viral Infection Maxime Leprêtre Nicole Faury Amélie Segarra Stéphane Claverol Lionel Degremont Mélissa Palos-Ladeiro Jean Armengaud Tristan Renault Benjamin Morga 2021-01-01T00:00:00Z https://doi.org/10.3389/fimmu.2020.621994 https://doaj.org/article/ad79cc3d48924a3a9b9555b70e349290 EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fimmu.2020.621994/full https://doaj.org/toc/1664-3224 1664-3224 doi:10.3389/fimmu.2020.621994 https://doaj.org/article/ad79cc3d48924a3a9b9555b70e349290 Frontiers in Immunology, Vol 11 (2021) proteomics interactions antiviral response OsHV-1 Pacific oysters Immunologic diseases. Allergy RC581-607 article 2021 ftdoajarticles https://doi.org/10.3389/fimmu.2020.621994 2022-12-31T07:41:34Z Massive mortality outbreaks affecting Pacific oysters (Crassostrea gigas) spat/juveniles are often associated with the detection of a herpesvirus called ostreid herpesvirus type 1 (OsHV-1). In this work, experimental infection trials of C. gigas spat with OsHV-1 were conducted using two contrasted Pacific oyster families for their susceptibility to viral infection. Live oysters were sampled at 12, 26, and 144 h post infection (hpi) to analyze host-pathogen interactions using comparative proteomics. Shotgun proteomics allowed the detection of seven viral proteins in infected oysters, some of them with potential immunomodulatoy functions. Viral proteins were mainly detected in susceptible oysters sampled at 26 hpi, which correlates with the mortality and viral load observed in this oyster family. Concerning the Pacific oyster proteome, more than 3,000 proteins were identified and contrasted proteomic responses were observed between infected A- and P-oysters, sampled at different post-injection times. Gene ontology (GO) and KEGG pathway enrichment analysis performed on significantly modulated proteins uncover the main immune processes (such as RNA interference, interferon-like pathway, antioxidant defense) which contribute to the defense and resistance of Pacific oysters to viral infection. In the more susceptible Pacific oysters, results suggest that OsHV-1 manipulate the molecular machinery of host immune response, in particular the autophagy system. This immunomodulation may lead to weakening and consecutively triggering death of Pacific oysters. The identification of several highly modulated and defense-related Pacific oyster proteins from the most resistant oysters supports the crucial role played by the innate immune system against OsHV-1 and the viral infection. Our results confirm the implication of proteins involved in an interferon-like pathway for efficient antiviral defenses and suggest that proteins involved in RNA interference process prevent viral replication in C. gigas. Overall, this study shows ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Directory of Open Access Journals: DOAJ Articles Pacific Frontiers in Immunology 11 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
proteomics interactions antiviral response OsHV-1 Pacific oysters Immunologic diseases. Allergy RC581-607 |
spellingShingle |
proteomics interactions antiviral response OsHV-1 Pacific oysters Immunologic diseases. Allergy RC581-607 Maxime Leprêtre Nicole Faury Amélie Segarra Stéphane Claverol Lionel Degremont Mélissa Palos-Ladeiro Jean Armengaud Tristan Renault Benjamin Morga Comparative Proteomics of Ostreid Herpesvirus 1 and Pacific Oyster Interactions With Two Families Exhibiting Contrasted Susceptibility to Viral Infection |
topic_facet |
proteomics interactions antiviral response OsHV-1 Pacific oysters Immunologic diseases. Allergy RC581-607 |
description |
Massive mortality outbreaks affecting Pacific oysters (Crassostrea gigas) spat/juveniles are often associated with the detection of a herpesvirus called ostreid herpesvirus type 1 (OsHV-1). In this work, experimental infection trials of C. gigas spat with OsHV-1 were conducted using two contrasted Pacific oyster families for their susceptibility to viral infection. Live oysters were sampled at 12, 26, and 144 h post infection (hpi) to analyze host-pathogen interactions using comparative proteomics. Shotgun proteomics allowed the detection of seven viral proteins in infected oysters, some of them with potential immunomodulatoy functions. Viral proteins were mainly detected in susceptible oysters sampled at 26 hpi, which correlates with the mortality and viral load observed in this oyster family. Concerning the Pacific oyster proteome, more than 3,000 proteins were identified and contrasted proteomic responses were observed between infected A- and P-oysters, sampled at different post-injection times. Gene ontology (GO) and KEGG pathway enrichment analysis performed on significantly modulated proteins uncover the main immune processes (such as RNA interference, interferon-like pathway, antioxidant defense) which contribute to the defense and resistance of Pacific oysters to viral infection. In the more susceptible Pacific oysters, results suggest that OsHV-1 manipulate the molecular machinery of host immune response, in particular the autophagy system. This immunomodulation may lead to weakening and consecutively triggering death of Pacific oysters. The identification of several highly modulated and defense-related Pacific oyster proteins from the most resistant oysters supports the crucial role played by the innate immune system against OsHV-1 and the viral infection. Our results confirm the implication of proteins involved in an interferon-like pathway for efficient antiviral defenses and suggest that proteins involved in RNA interference process prevent viral replication in C. gigas. Overall, this study shows ... |
format |
Article in Journal/Newspaper |
author |
Maxime Leprêtre Nicole Faury Amélie Segarra Stéphane Claverol Lionel Degremont Mélissa Palos-Ladeiro Jean Armengaud Tristan Renault Benjamin Morga |
author_facet |
Maxime Leprêtre Nicole Faury Amélie Segarra Stéphane Claverol Lionel Degremont Mélissa Palos-Ladeiro Jean Armengaud Tristan Renault Benjamin Morga |
author_sort |
Maxime Leprêtre |
title |
Comparative Proteomics of Ostreid Herpesvirus 1 and Pacific Oyster Interactions With Two Families Exhibiting Contrasted Susceptibility to Viral Infection |
title_short |
Comparative Proteomics of Ostreid Herpesvirus 1 and Pacific Oyster Interactions With Two Families Exhibiting Contrasted Susceptibility to Viral Infection |
title_full |
Comparative Proteomics of Ostreid Herpesvirus 1 and Pacific Oyster Interactions With Two Families Exhibiting Contrasted Susceptibility to Viral Infection |
title_fullStr |
Comparative Proteomics of Ostreid Herpesvirus 1 and Pacific Oyster Interactions With Two Families Exhibiting Contrasted Susceptibility to Viral Infection |
title_full_unstemmed |
Comparative Proteomics of Ostreid Herpesvirus 1 and Pacific Oyster Interactions With Two Families Exhibiting Contrasted Susceptibility to Viral Infection |
title_sort |
comparative proteomics of ostreid herpesvirus 1 and pacific oyster interactions with two families exhibiting contrasted susceptibility to viral infection |
publisher |
Frontiers Media S.A. |
publishDate |
2021 |
url |
https://doi.org/10.3389/fimmu.2020.621994 https://doaj.org/article/ad79cc3d48924a3a9b9555b70e349290 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Frontiers in Immunology, Vol 11 (2021) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fimmu.2020.621994/full https://doaj.org/toc/1664-3224 1664-3224 doi:10.3389/fimmu.2020.621994 https://doaj.org/article/ad79cc3d48924a3a9b9555b70e349290 |
op_doi |
https://doi.org/10.3389/fimmu.2020.621994 |
container_title |
Frontiers in Immunology |
container_volume |
11 |
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1766394848521224192 |