Retrospective Enhanced Bat Lyssavirus Surveillance in Germany between 2018–2020
Lyssaviruses are the causative agents for rabies, a zoonotic and fatal disease. Bats are the ancestral reservoir host for lyssaviruses, and at least three different lyssaviruses have been found in bats from Germany. Across Europe, novel lyssaviruses were identified in bats recently and occasional sp...
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ftdoajarticles:oai:doaj.org/article:ad746c86f23b4ef09a09c38e155d1c27 2023-05-15T17:13:46+02:00 Retrospective Enhanced Bat Lyssavirus Surveillance in Germany between 2018–2020 Antonia Klein Sten Calvelage Kore Schlottau Bernd Hoffmann Elisa Eggerbauer Thomas Müller Conrad M. Freuling 2021-08-01T00:00:00Z https://doi.org/10.3390/v13081538 https://doaj.org/article/ad746c86f23b4ef09a09c38e155d1c27 EN eng MDPI AG https://www.mdpi.com/1999-4915/13/8/1538 https://doaj.org/toc/1999-4915 doi:10.3390/v13081538 1999-4915 https://doaj.org/article/ad746c86f23b4ef09a09c38e155d1c27 Viruses, Vol 13, Iss 1538, p 1538 (2021) bat lyssavirus bat rabies surveillance European bat lyssavirus 1 (EBLV-1) Bokeloh bat lyssavirus (BBLV) zoonosis Microbiology QR1-502 article 2021 ftdoajarticles https://doi.org/10.3390/v13081538 2022-12-31T04:28:55Z Lyssaviruses are the causative agents for rabies, a zoonotic and fatal disease. Bats are the ancestral reservoir host for lyssaviruses, and at least three different lyssaviruses have been found in bats from Germany. Across Europe, novel lyssaviruses were identified in bats recently and occasional spillover infections in other mammals and human cases highlight their public health relevance. Here, we report the results from an enhanced passive bat rabies surveillance that encompasses samples without human contact that would not be tested under routine conditions. To this end, 1236 bat brain samples obtained between 2018 and 2020 were screened for lyssaviruses via several RT-qPCR assays. European bat lyssavirus type 1 (EBLV-1) was dominant, with 15 positives exclusively found in serotine bats ( Eptesicus serotinus ) from northern Germany. Additionally, when an archived set of bat samples that had tested negative for rabies by the FAT were screened in the process of assay validation, four samples tested EBLV-1 positive, including two detected in Pipistrellus pipistrellus . Subsequent phylogenetic analysis of 17 full genomes assigned all except one of these viruses to the A1 cluster of the EBLV-1a sub-lineage. Furthermore, we report here another Bokeloh bat lyssavirus (BBLV) infection in a Natterer’s bat ( Myotis nattereri ) found in Lower Saxony, the tenth reported case of this novel bat lyssavirus. Article in Journal/Newspaper Myotis nattereri Pipistrellus pipistrellus Directory of Open Access Journals: DOAJ Articles Viruses 13 8 1538 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
bat lyssavirus bat rabies surveillance European bat lyssavirus 1 (EBLV-1) Bokeloh bat lyssavirus (BBLV) zoonosis Microbiology QR1-502 |
spellingShingle |
bat lyssavirus bat rabies surveillance European bat lyssavirus 1 (EBLV-1) Bokeloh bat lyssavirus (BBLV) zoonosis Microbiology QR1-502 Antonia Klein Sten Calvelage Kore Schlottau Bernd Hoffmann Elisa Eggerbauer Thomas Müller Conrad M. Freuling Retrospective Enhanced Bat Lyssavirus Surveillance in Germany between 2018–2020 |
topic_facet |
bat lyssavirus bat rabies surveillance European bat lyssavirus 1 (EBLV-1) Bokeloh bat lyssavirus (BBLV) zoonosis Microbiology QR1-502 |
description |
Lyssaviruses are the causative agents for rabies, a zoonotic and fatal disease. Bats are the ancestral reservoir host for lyssaviruses, and at least three different lyssaviruses have been found in bats from Germany. Across Europe, novel lyssaviruses were identified in bats recently and occasional spillover infections in other mammals and human cases highlight their public health relevance. Here, we report the results from an enhanced passive bat rabies surveillance that encompasses samples without human contact that would not be tested under routine conditions. To this end, 1236 bat brain samples obtained between 2018 and 2020 were screened for lyssaviruses via several RT-qPCR assays. European bat lyssavirus type 1 (EBLV-1) was dominant, with 15 positives exclusively found in serotine bats ( Eptesicus serotinus ) from northern Germany. Additionally, when an archived set of bat samples that had tested negative for rabies by the FAT were screened in the process of assay validation, four samples tested EBLV-1 positive, including two detected in Pipistrellus pipistrellus . Subsequent phylogenetic analysis of 17 full genomes assigned all except one of these viruses to the A1 cluster of the EBLV-1a sub-lineage. Furthermore, we report here another Bokeloh bat lyssavirus (BBLV) infection in a Natterer’s bat ( Myotis nattereri ) found in Lower Saxony, the tenth reported case of this novel bat lyssavirus. |
format |
Article in Journal/Newspaper |
author |
Antonia Klein Sten Calvelage Kore Schlottau Bernd Hoffmann Elisa Eggerbauer Thomas Müller Conrad M. Freuling |
author_facet |
Antonia Klein Sten Calvelage Kore Schlottau Bernd Hoffmann Elisa Eggerbauer Thomas Müller Conrad M. Freuling |
author_sort |
Antonia Klein |
title |
Retrospective Enhanced Bat Lyssavirus Surveillance in Germany between 2018–2020 |
title_short |
Retrospective Enhanced Bat Lyssavirus Surveillance in Germany between 2018–2020 |
title_full |
Retrospective Enhanced Bat Lyssavirus Surveillance in Germany between 2018–2020 |
title_fullStr |
Retrospective Enhanced Bat Lyssavirus Surveillance in Germany between 2018–2020 |
title_full_unstemmed |
Retrospective Enhanced Bat Lyssavirus Surveillance in Germany between 2018–2020 |
title_sort |
retrospective enhanced bat lyssavirus surveillance in germany between 2018–2020 |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/v13081538 https://doaj.org/article/ad746c86f23b4ef09a09c38e155d1c27 |
genre |
Myotis nattereri Pipistrellus pipistrellus |
genre_facet |
Myotis nattereri Pipistrellus pipistrellus |
op_source |
Viruses, Vol 13, Iss 1538, p 1538 (2021) |
op_relation |
https://www.mdpi.com/1999-4915/13/8/1538 https://doaj.org/toc/1999-4915 doi:10.3390/v13081538 1999-4915 https://doaj.org/article/ad746c86f23b4ef09a09c38e155d1c27 |
op_doi |
https://doi.org/10.3390/v13081538 |
container_title |
Viruses |
container_volume |
13 |
container_issue |
8 |
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1538 |
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1766070951923941376 |