Elaborate evaluation of serum and tissue oxidized LDL level with darapladib therapy: A feasible diagnostic marker for early atherogenesis
Objective: To compare oxidized low density lipoprotein (oxLDL) levels in serum and vascular wall of Sprague-Dawley rats, identify their patterns in 8 weeks and 16 weeks of dyslipidemia induced by high fat diet, compare foam cells in aorta of each group and investigate lipoprotein-associated phosphol...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wolters Kluwer Medknow Publications
2017
|
Subjects: | |
Online Access: | https://doi.org/10.1016/j.apjtb.2016.11.014 https://doaj.org/article/ad67c055c1d4413f8b9e93c1941065c5 |
id |
ftdoajarticles:oai:doaj.org/article:ad67c055c1d4413f8b9e93c1941065c5 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:ad67c055c1d4413f8b9e93c1941065c5 2023-05-15T15:14:16+02:00 Elaborate evaluation of serum and tissue oxidized LDL level with darapladib therapy: A feasible diagnostic marker for early atherogenesis Teuku Heriansyah Aditya Angela Adam Titin Andri Wihastuti Mohammad Saifur Rohman 2017-02-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2016.11.014 https://doaj.org/article/ad67c055c1d4413f8b9e93c1941065c5 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169116300983 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.11.014 https://doaj.org/article/ad67c055c1d4413f8b9e93c1941065c5 Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 2, Pp 134-138 (2017) OxLDL Serum Aorta Lp-PLA2 Darapladib Foam cell Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2017 ftdoajarticles https://doi.org/10.1016/j.apjtb.2016.11.014 2022-12-30T21:26:57Z Objective: To compare oxidized low density lipoprotein (oxLDL) levels in serum and vascular wall of Sprague-Dawley rats, identify their patterns in 8 weeks and 16 weeks of dyslipidemia induced by high fat diet, compare foam cells in aorta of each group and investigate lipoprotein-associated phospholipase A2 (Lp-PLA2) role in atherosclerosis by darapladib administration. Methods: This study generated in twenty-four Sprague-Dawley rats. Rats were divided into 6 groups, which were received normal diet (normal group), high fat diet and high fat diet plus darapladib therapy for both 8 weeks and 16 weeks. Surgeries were performed at Week 8 and Week 16 to take the blood serum and aortic tissue. Level of oxLDL in serum, oxLDL aortic tissue, foam cell amount in aortic tissue, and Lp-PLA2 expression in aortic tissue were measured. Results: There were significant differences in oxLDL level in serum, aortic tissue and foam cell amount (P < 0.05). There was no significant difference in Lp-PLA2 expression in aortic tissue. OxLDL in serum and aortic tissue had a very strong correlation (r2 > 0.9, P < 0.05). This study also composed an equation for oxLDL level in aortic tissue prediction. Factorial ANOVA found that there was a significant difference of oxLDL level in the interactions between duration and location, location and treatment, and also duration, location and treatment (P < 0.01). Administration of darapladib was able to reduce levels of oxLDL in serum, aortic tissue and foam cell significantly (P < 0.05, P < 0.05 and P < 0.01, subsequently). Conclusions: OxLDL level is location-dependent and duration-dependent. As a feasible early diagnosis, we can predict oxLDL level in aortic tissue by its level in serum. Though Lp-PLA2 expression was unsignificant, Lp-PLA2 inhibition by darapladib can reduce oxidative stress and inflammation in atherogenesis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 7 2 134 138 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
OxLDL Serum Aorta Lp-PLA2 Darapladib Foam cell Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
OxLDL Serum Aorta Lp-PLA2 Darapladib Foam cell Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Teuku Heriansyah Aditya Angela Adam Titin Andri Wihastuti Mohammad Saifur Rohman Elaborate evaluation of serum and tissue oxidized LDL level with darapladib therapy: A feasible diagnostic marker for early atherogenesis |
topic_facet |
OxLDL Serum Aorta Lp-PLA2 Darapladib Foam cell Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To compare oxidized low density lipoprotein (oxLDL) levels in serum and vascular wall of Sprague-Dawley rats, identify their patterns in 8 weeks and 16 weeks of dyslipidemia induced by high fat diet, compare foam cells in aorta of each group and investigate lipoprotein-associated phospholipase A2 (Lp-PLA2) role in atherosclerosis by darapladib administration. Methods: This study generated in twenty-four Sprague-Dawley rats. Rats were divided into 6 groups, which were received normal diet (normal group), high fat diet and high fat diet plus darapladib therapy for both 8 weeks and 16 weeks. Surgeries were performed at Week 8 and Week 16 to take the blood serum and aortic tissue. Level of oxLDL in serum, oxLDL aortic tissue, foam cell amount in aortic tissue, and Lp-PLA2 expression in aortic tissue were measured. Results: There were significant differences in oxLDL level in serum, aortic tissue and foam cell amount (P < 0.05). There was no significant difference in Lp-PLA2 expression in aortic tissue. OxLDL in serum and aortic tissue had a very strong correlation (r2 > 0.9, P < 0.05). This study also composed an equation for oxLDL level in aortic tissue prediction. Factorial ANOVA found that there was a significant difference of oxLDL level in the interactions between duration and location, location and treatment, and also duration, location and treatment (P < 0.01). Administration of darapladib was able to reduce levels of oxLDL in serum, aortic tissue and foam cell significantly (P < 0.05, P < 0.05 and P < 0.01, subsequently). Conclusions: OxLDL level is location-dependent and duration-dependent. As a feasible early diagnosis, we can predict oxLDL level in aortic tissue by its level in serum. Though Lp-PLA2 expression was unsignificant, Lp-PLA2 inhibition by darapladib can reduce oxidative stress and inflammation in atherogenesis. |
format |
Article in Journal/Newspaper |
author |
Teuku Heriansyah Aditya Angela Adam Titin Andri Wihastuti Mohammad Saifur Rohman |
author_facet |
Teuku Heriansyah Aditya Angela Adam Titin Andri Wihastuti Mohammad Saifur Rohman |
author_sort |
Teuku Heriansyah |
title |
Elaborate evaluation of serum and tissue oxidized LDL level with darapladib therapy: A feasible diagnostic marker for early atherogenesis |
title_short |
Elaborate evaluation of serum and tissue oxidized LDL level with darapladib therapy: A feasible diagnostic marker for early atherogenesis |
title_full |
Elaborate evaluation of serum and tissue oxidized LDL level with darapladib therapy: A feasible diagnostic marker for early atherogenesis |
title_fullStr |
Elaborate evaluation of serum and tissue oxidized LDL level with darapladib therapy: A feasible diagnostic marker for early atherogenesis |
title_full_unstemmed |
Elaborate evaluation of serum and tissue oxidized LDL level with darapladib therapy: A feasible diagnostic marker for early atherogenesis |
title_sort |
elaborate evaluation of serum and tissue oxidized ldl level with darapladib therapy: a feasible diagnostic marker for early atherogenesis |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2017 |
url |
https://doi.org/10.1016/j.apjtb.2016.11.014 https://doaj.org/article/ad67c055c1d4413f8b9e93c1941065c5 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 2, Pp 134-138 (2017) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169116300983 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.11.014 https://doaj.org/article/ad67c055c1d4413f8b9e93c1941065c5 |
op_doi |
https://doi.org/10.1016/j.apjtb.2016.11.014 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
7 |
container_issue |
2 |
container_start_page |
134 |
op_container_end_page |
138 |
_version_ |
1766344736668385280 |