Molecular and Structural Characterization of MHC Class II β Genes Reveals High Diversity in the Cold-Adapted Icefish Chionodraco hamatus
Abstract This study reports the presence of two distinct MHC class II β genes in the Antarctic icefish Chionodraco hamatus, belonging to the classical (ChhaDAB) and nonclassical (ChhaDBB) evolutionary lineages. By the application of targeted sequencing approach, a remarkable molecular diversity in t...
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ftdoajarticles:oai:doaj.org/article:ad64b1070fe44265ab097d244ba6a0b5 2023-05-15T13:52:07+02:00 Molecular and Structural Characterization of MHC Class II β Genes Reveals High Diversity in the Cold-Adapted Icefish Chionodraco hamatus Marco Gerdol Daniela Lucente Francesco Buonocore Elia Poerio Giuseppe Scapigliati Simonetta Mattiucci Alberto Pallavicini Roberta Cimmaruta 2019-04-01T00:00:00Z https://doi.org/10.1038/s41598-019-42003-5 https://doaj.org/article/ad64b1070fe44265ab097d244ba6a0b5 EN eng Nature Portfolio https://doi.org/10.1038/s41598-019-42003-5 https://doaj.org/toc/2045-2322 doi:10.1038/s41598-019-42003-5 2045-2322 https://doaj.org/article/ad64b1070fe44265ab097d244ba6a0b5 Scientific Reports, Vol 9, Iss 1, Pp 1-14 (2019) Medicine R Science Q article 2019 ftdoajarticles https://doi.org/10.1038/s41598-019-42003-5 2022-12-31T08:59:39Z Abstract This study reports the presence of two distinct MHC class II β genes in the Antarctic icefish Chionodraco hamatus, belonging to the classical (ChhaDAB) and nonclassical (ChhaDBB) evolutionary lineages. By the application of targeted sequencing approach, a remarkable molecular diversity in the exon 2 sequence of the highly expressed gene ChhaDAB has been observed, resulting in an estimate of 92 different variants translated in 87 different peptides from 54 analysed icefish individuals. A highly conservative estimate, based on a 95% sequence identity threshold clustering, translate this variability in 41 different peptide clusters belonging to four different clades and showing the signature of different kinds of selection. In stark contrast, the poorly expressed ChhaDBB gene displayed a very low level of molecular diversity within exon 2, in agreement with expectations for a nonclassical MHC class II β gene. Article in Journal/Newspaper Antarc* Antarctic Icefish Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic Scientific Reports 9 1 |
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Directory of Open Access Journals: DOAJ Articles |
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topic |
Medicine R Science Q |
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Medicine R Science Q Marco Gerdol Daniela Lucente Francesco Buonocore Elia Poerio Giuseppe Scapigliati Simonetta Mattiucci Alberto Pallavicini Roberta Cimmaruta Molecular and Structural Characterization of MHC Class II β Genes Reveals High Diversity in the Cold-Adapted Icefish Chionodraco hamatus |
topic_facet |
Medicine R Science Q |
description |
Abstract This study reports the presence of two distinct MHC class II β genes in the Antarctic icefish Chionodraco hamatus, belonging to the classical (ChhaDAB) and nonclassical (ChhaDBB) evolutionary lineages. By the application of targeted sequencing approach, a remarkable molecular diversity in the exon 2 sequence of the highly expressed gene ChhaDAB has been observed, resulting in an estimate of 92 different variants translated in 87 different peptides from 54 analysed icefish individuals. A highly conservative estimate, based on a 95% sequence identity threshold clustering, translate this variability in 41 different peptide clusters belonging to four different clades and showing the signature of different kinds of selection. In stark contrast, the poorly expressed ChhaDBB gene displayed a very low level of molecular diversity within exon 2, in agreement with expectations for a nonclassical MHC class II β gene. |
format |
Article in Journal/Newspaper |
author |
Marco Gerdol Daniela Lucente Francesco Buonocore Elia Poerio Giuseppe Scapigliati Simonetta Mattiucci Alberto Pallavicini Roberta Cimmaruta |
author_facet |
Marco Gerdol Daniela Lucente Francesco Buonocore Elia Poerio Giuseppe Scapigliati Simonetta Mattiucci Alberto Pallavicini Roberta Cimmaruta |
author_sort |
Marco Gerdol |
title |
Molecular and Structural Characterization of MHC Class II β Genes Reveals High Diversity in the Cold-Adapted Icefish Chionodraco hamatus |
title_short |
Molecular and Structural Characterization of MHC Class II β Genes Reveals High Diversity in the Cold-Adapted Icefish Chionodraco hamatus |
title_full |
Molecular and Structural Characterization of MHC Class II β Genes Reveals High Diversity in the Cold-Adapted Icefish Chionodraco hamatus |
title_fullStr |
Molecular and Structural Characterization of MHC Class II β Genes Reveals High Diversity in the Cold-Adapted Icefish Chionodraco hamatus |
title_full_unstemmed |
Molecular and Structural Characterization of MHC Class II β Genes Reveals High Diversity in the Cold-Adapted Icefish Chionodraco hamatus |
title_sort |
molecular and structural characterization of mhc class ii β genes reveals high diversity in the cold-adapted icefish chionodraco hamatus |
publisher |
Nature Portfolio |
publishDate |
2019 |
url |
https://doi.org/10.1038/s41598-019-42003-5 https://doaj.org/article/ad64b1070fe44265ab097d244ba6a0b5 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Icefish |
genre_facet |
Antarc* Antarctic Icefish |
op_source |
Scientific Reports, Vol 9, Iss 1, Pp 1-14 (2019) |
op_relation |
https://doi.org/10.1038/s41598-019-42003-5 https://doaj.org/toc/2045-2322 doi:10.1038/s41598-019-42003-5 2045-2322 https://doaj.org/article/ad64b1070fe44265ab097d244ba6a0b5 |
op_doi |
https://doi.org/10.1038/s41598-019-42003-5 |
container_title |
Scientific Reports |
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9 |
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1 |
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1766256362496458752 |