Serodiagnosis of Echinococcus spp. infection: explorative selection of diagnostic antigens by peptide microarray.
BACKGROUND: Production of native antigens for serodiagnosis of helminthic infections is laborious and hampered by batch-to-batch variation. For serodiagnosis of echinococcosis, especially cystic disease, most screening tests rely on crude or purified Echinococcus granulosus hydatid cyst fluid. To re...
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ftdoajarticles:oai:doaj.org/article:ad600063c11143b0b04c414d176459e8 2023-05-15T15:13:48+02:00 Serodiagnosis of Echinococcus spp. infection: explorative selection of diagnostic antigens by peptide microarray. Claudia List Weihong Qi Eva Maag Bruno Gottstein Norbert Müller Ingrid Felger 2010-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0000771 https://doaj.org/article/ad600063c11143b0b04c414d176459e8 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC2914747?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0000771 https://doaj.org/article/ad600063c11143b0b04c414d176459e8 PLoS Neglected Tropical Diseases, Vol 4, Iss 8, p e771 (2010) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2010 ftdoajarticles https://doi.org/10.1371/journal.pntd.0000771 2022-12-31T16:23:01Z BACKGROUND: Production of native antigens for serodiagnosis of helminthic infections is laborious and hampered by batch-to-batch variation. For serodiagnosis of echinococcosis, especially cystic disease, most screening tests rely on crude or purified Echinococcus granulosus hydatid cyst fluid. To resolve limitations associated with native antigens in serological tests, the use of standardized and highly pure antigens produced by chemical synthesis offers considerable advantages, provided appropriate diagnostic sensitivity and specificity is achieved. METHODOLOGY/PRINCIPAL FINDINGS: Making use of the growing collection of genomic and proteomic data, we applied a set of bioinformatic selection criteria to a collection of protein sequences including conceptually translated nucleotide sequence data of two related tapeworms, Echinococcus multilocularis and Echinococcus granulosus. Our approach targeted alpha-helical coiled-coils and intrinsically unstructured regions of parasite proteins potentially exposed to the host immune system. From 6 proteins of E. multilocularis and 5 proteins of E. granulosus, 45 peptides between 24 and 30 amino acids in length were designed. These peptides were chemically synthesized, spotted on microarrays and screened for reactivity with sera from infected humans. Peptides reacting above the cut-off were validated in enzyme-linked immunosorbent assays (ELISA). Peptides identified failed to differentiate between E. multilocularis and E. granulosus infection. The peptide performing best reached 57% sensitivity and 94% specificity. This candidate derived from Echinococcus multilocularis antigen B8/1 and showed strong reactivity to sera from patients infected either with E. multilocularis or E. granulosus. CONCLUSIONS/SIGNIFICANCE: This study provides proof of principle for the discovery of diagnostically relevant peptides by bioinformatic selection complemented with screening on a high-throughput microarray platform. Our data showed that a single peptide cannot provide sufficient diagnostic ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 4 8 e771 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Claudia List Weihong Qi Eva Maag Bruno Gottstein Norbert Müller Ingrid Felger Serodiagnosis of Echinococcus spp. infection: explorative selection of diagnostic antigens by peptide microarray. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND: Production of native antigens for serodiagnosis of helminthic infections is laborious and hampered by batch-to-batch variation. For serodiagnosis of echinococcosis, especially cystic disease, most screening tests rely on crude or purified Echinococcus granulosus hydatid cyst fluid. To resolve limitations associated with native antigens in serological tests, the use of standardized and highly pure antigens produced by chemical synthesis offers considerable advantages, provided appropriate diagnostic sensitivity and specificity is achieved. METHODOLOGY/PRINCIPAL FINDINGS: Making use of the growing collection of genomic and proteomic data, we applied a set of bioinformatic selection criteria to a collection of protein sequences including conceptually translated nucleotide sequence data of two related tapeworms, Echinococcus multilocularis and Echinococcus granulosus. Our approach targeted alpha-helical coiled-coils and intrinsically unstructured regions of parasite proteins potentially exposed to the host immune system. From 6 proteins of E. multilocularis and 5 proteins of E. granulosus, 45 peptides between 24 and 30 amino acids in length were designed. These peptides were chemically synthesized, spotted on microarrays and screened for reactivity with sera from infected humans. Peptides reacting above the cut-off were validated in enzyme-linked immunosorbent assays (ELISA). Peptides identified failed to differentiate between E. multilocularis and E. granulosus infection. The peptide performing best reached 57% sensitivity and 94% specificity. This candidate derived from Echinococcus multilocularis antigen B8/1 and showed strong reactivity to sera from patients infected either with E. multilocularis or E. granulosus. CONCLUSIONS/SIGNIFICANCE: This study provides proof of principle for the discovery of diagnostically relevant peptides by bioinformatic selection complemented with screening on a high-throughput microarray platform. Our data showed that a single peptide cannot provide sufficient diagnostic ... |
format |
Article in Journal/Newspaper |
author |
Claudia List Weihong Qi Eva Maag Bruno Gottstein Norbert Müller Ingrid Felger |
author_facet |
Claudia List Weihong Qi Eva Maag Bruno Gottstein Norbert Müller Ingrid Felger |
author_sort |
Claudia List |
title |
Serodiagnosis of Echinococcus spp. infection: explorative selection of diagnostic antigens by peptide microarray. |
title_short |
Serodiagnosis of Echinococcus spp. infection: explorative selection of diagnostic antigens by peptide microarray. |
title_full |
Serodiagnosis of Echinococcus spp. infection: explorative selection of diagnostic antigens by peptide microarray. |
title_fullStr |
Serodiagnosis of Echinococcus spp. infection: explorative selection of diagnostic antigens by peptide microarray. |
title_full_unstemmed |
Serodiagnosis of Echinococcus spp. infection: explorative selection of diagnostic antigens by peptide microarray. |
title_sort |
serodiagnosis of echinococcus spp. infection: explorative selection of diagnostic antigens by peptide microarray. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2010 |
url |
https://doi.org/10.1371/journal.pntd.0000771 https://doaj.org/article/ad600063c11143b0b04c414d176459e8 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 4, Iss 8, p e771 (2010) |
op_relation |
http://europepmc.org/articles/PMC2914747?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0000771 https://doaj.org/article/ad600063c11143b0b04c414d176459e8 |
op_doi |
https://doi.org/10.1371/journal.pntd.0000771 |
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PLoS Neglected Tropical Diseases |
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4 |
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8 |
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e771 |
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