Specific IgY anti-group 1 dust mite allergens induced by unglycosylated synthetic oligopeptides
Introduction: The use of specific antibodies capable of detecting allergens of the group 1 of house dust mites represents a potential strategy to reduce exposure and clinical symptomatology associated with asthma and allergic rhinitis. Objective: To produce and purify chicken antibodies specific for...
Published in: | Biomédica |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English Spanish |
Published: |
Instituto Nacional de Salud
2018
|
Subjects: | |
Online Access: | https://doi.org/10.7705/biomedica.v38i0.3689 https://doaj.org/article/ad3dd29ffe0c43c9a7ee51ca614dd3d0 |
id |
ftdoajarticles:oai:doaj.org/article:ad3dd29ffe0c43c9a7ee51ca614dd3d0 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:ad3dd29ffe0c43c9a7ee51ca614dd3d0 2023-05-15T15:09:28+02:00 Specific IgY anti-group 1 dust mite allergens induced by unglycosylated synthetic oligopeptides Eduardo Egea Dary Mendoza Gloria Garavito Ángela Espejo Lina Maria Lizaraso Elkin Navarro Luis Alejandro Barrera 2018-06-01T00:00:00Z https://doi.org/10.7705/biomedica.v38i0.3689 https://doaj.org/article/ad3dd29ffe0c43c9a7ee51ca614dd3d0 EN ES eng spa Instituto Nacional de Salud https://www.revistabiomedica.org/index.php/biomedica/article/view/3689 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.v38i0.3689 https://doaj.org/article/ad3dd29ffe0c43c9a7ee51ca614dd3d0 Biomédica: revista del Instituto Nacional de Salud, Vol 38, Iss 2, Pp 232-243 (2018) alérgenos anticuerpos ácaros oligopéptidos yema de huevo Pyroglyphidae Medicine R Arctic medicine. Tropical medicine RC955-962 article 2018 ftdoajarticles https://doi.org/10.7705/biomedica.v38i0.3689 2022-12-31T00:47:12Z Introduction: The use of specific antibodies capable of detecting allergens of the group 1 of house dust mites represents a potential strategy to reduce exposure and clinical symptomatology associated with asthma and allergic rhinitis. Objective: To produce and purify chicken antibodies specific for the dust mites Dermatophagoides sp. and B. tropicalis using the IgY technology. Materials and methods: We designed and synthesized oligopeptides showing immunogenic epitopes of Der p1, Der f1, and Blo t1. These were used to produce IgY antibodies in Hy Line Brown chickens. IgY were extracted from egg yolk using thiophilic chromatography. The immunogenicity and specificity were assayed by indirect ELISA and Dot Blot. Results: We obtained high reactivity of IgY antibodies against epitopes of allergens present in whole body mites extracts of D. farinae, D. pteronyssinus, and B. tropicalis. The highest IgY levels were registered between days 32 and 40 after immunization. The antibodies showed high immunoreactivity and specificity towards D. farinae proteins with detection limits above 0.03 μg of mite proteins under the experimental conditions used. Purified IgY did not show significant reactivity when binding to Periplaneta americana extract. Conclusion: The IgY technology allowed the production of specific antibodies against house dust mites group 1 allergens using non-glycosylated synthetic peptides. To our knowledge, this is the first time that this immunochemicals are used in the detection of mites of medical relevance. Article in Journal/Newspaper Arctic Mite Directory of Open Access Journals: DOAJ Articles Arctic Huevo ENVELOPE(-57.733,-57.733,-63.683,-63.683) Biomédica 38 2 232 243 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Spanish |
topic |
alérgenos anticuerpos ácaros oligopéptidos yema de huevo Pyroglyphidae Medicine R Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
alérgenos anticuerpos ácaros oligopéptidos yema de huevo Pyroglyphidae Medicine R Arctic medicine. Tropical medicine RC955-962 Eduardo Egea Dary Mendoza Gloria Garavito Ángela Espejo Lina Maria Lizaraso Elkin Navarro Luis Alejandro Barrera Specific IgY anti-group 1 dust mite allergens induced by unglycosylated synthetic oligopeptides |
topic_facet |
alérgenos anticuerpos ácaros oligopéptidos yema de huevo Pyroglyphidae Medicine R Arctic medicine. Tropical medicine RC955-962 |
description |
Introduction: The use of specific antibodies capable of detecting allergens of the group 1 of house dust mites represents a potential strategy to reduce exposure and clinical symptomatology associated with asthma and allergic rhinitis. Objective: To produce and purify chicken antibodies specific for the dust mites Dermatophagoides sp. and B. tropicalis using the IgY technology. Materials and methods: We designed and synthesized oligopeptides showing immunogenic epitopes of Der p1, Der f1, and Blo t1. These were used to produce IgY antibodies in Hy Line Brown chickens. IgY were extracted from egg yolk using thiophilic chromatography. The immunogenicity and specificity were assayed by indirect ELISA and Dot Blot. Results: We obtained high reactivity of IgY antibodies against epitopes of allergens present in whole body mites extracts of D. farinae, D. pteronyssinus, and B. tropicalis. The highest IgY levels were registered between days 32 and 40 after immunization. The antibodies showed high immunoreactivity and specificity towards D. farinae proteins with detection limits above 0.03 μg of mite proteins under the experimental conditions used. Purified IgY did not show significant reactivity when binding to Periplaneta americana extract. Conclusion: The IgY technology allowed the production of specific antibodies against house dust mites group 1 allergens using non-glycosylated synthetic peptides. To our knowledge, this is the first time that this immunochemicals are used in the detection of mites of medical relevance. |
format |
Article in Journal/Newspaper |
author |
Eduardo Egea Dary Mendoza Gloria Garavito Ángela Espejo Lina Maria Lizaraso Elkin Navarro Luis Alejandro Barrera |
author_facet |
Eduardo Egea Dary Mendoza Gloria Garavito Ángela Espejo Lina Maria Lizaraso Elkin Navarro Luis Alejandro Barrera |
author_sort |
Eduardo Egea |
title |
Specific IgY anti-group 1 dust mite allergens induced by unglycosylated synthetic oligopeptides |
title_short |
Specific IgY anti-group 1 dust mite allergens induced by unglycosylated synthetic oligopeptides |
title_full |
Specific IgY anti-group 1 dust mite allergens induced by unglycosylated synthetic oligopeptides |
title_fullStr |
Specific IgY anti-group 1 dust mite allergens induced by unglycosylated synthetic oligopeptides |
title_full_unstemmed |
Specific IgY anti-group 1 dust mite allergens induced by unglycosylated synthetic oligopeptides |
title_sort |
specific igy anti-group 1 dust mite allergens induced by unglycosylated synthetic oligopeptides |
publisher |
Instituto Nacional de Salud |
publishDate |
2018 |
url |
https://doi.org/10.7705/biomedica.v38i0.3689 https://doaj.org/article/ad3dd29ffe0c43c9a7ee51ca614dd3d0 |
long_lat |
ENVELOPE(-57.733,-57.733,-63.683,-63.683) |
geographic |
Arctic Huevo |
geographic_facet |
Arctic Huevo |
genre |
Arctic Mite |
genre_facet |
Arctic Mite |
op_source |
Biomédica: revista del Instituto Nacional de Salud, Vol 38, Iss 2, Pp 232-243 (2018) |
op_relation |
https://www.revistabiomedica.org/index.php/biomedica/article/view/3689 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.v38i0.3689 https://doaj.org/article/ad3dd29ffe0c43c9a7ee51ca614dd3d0 |
op_doi |
https://doi.org/10.7705/biomedica.v38i0.3689 |
container_title |
Biomédica |
container_volume |
38 |
container_issue |
2 |
container_start_page |
232 |
op_container_end_page |
243 |
_version_ |
1766340658607423488 |