Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination
The adjuvant effect of Quillaja saponaria saponin (QSS) on protection of turbot fry was investigated with immersion vaccination of formalin-killed Vibrio anguillarum O1 and various concentrations of QSS (5, 25, 45 and 65 mg/L). Fish were challenged at days 7, 14 and 28 post-vaccination. Significantl...
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ftdoajarticles:oai:doaj.org/article:ad1acd5bf97b4d34b0fb39038159cd4f 2023-05-15T18:15:44+02:00 Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination Yujuan Wang Xiuhua Wang Jie Huang Jun Li 2016-03-01T00:00:00Z https://doi.org/10.3390/ijms17030325 https://doaj.org/article/ad1acd5bf97b4d34b0fb39038159cd4f EN eng MDPI AG http://www.mdpi.com/1422-0067/17/3/325 https://doaj.org/toc/1422-0067 1422-0067 doi:10.3390/ijms17030325 https://doaj.org/article/ad1acd5bf97b4d34b0fb39038159cd4f International Journal of Molecular Sciences, Vol 17, Iss 3, p 325 (2016) Quillaja saponaria saponins adjuvant vaccination Scophthalmus maximus relative percent of survival (RPS) Biology (General) QH301-705.5 Chemistry QD1-999 article 2016 ftdoajarticles https://doi.org/10.3390/ijms17030325 2022-12-31T02:56:38Z The adjuvant effect of Quillaja saponaria saponin (QSS) on protection of turbot fry was investigated with immersion vaccination of formalin-killed Vibrio anguillarum O1 and various concentrations of QSS (5, 25, 45 and 65 mg/L). Fish were challenged at days 7, 14 and 28 post-vaccination. Significantly high relative percent of survival (RPS) ((59.1 ± 13.6)%, (81.7 ± 8.2)%, (77.8 ± 9.6)%) were recorded in the fish that received bacterins immersion with QSS at 45 mg/L, which is comparable to the positive control group vaccinated by intraperitoneal injection (IP). Moreover, a remarkably higher serum antibody titer was also demonstrated after 28 days in the vaccinated fish with QSS (45 mg/L) than those vaccinated fish without QSS (p < 0.05), but lower than the IP immunized fish (p < 0.05). Significant upregulation of IgM gene expression has also been identified in the tissues of skin, gill, spleen and kidney from the immunized fish in comparison to the control fish. Taken together, the present study indicated that QSS was able to dramatically evoke systemic and mucosal immune responses in immunized fish. Therefore, QSS might be a promising adjuvant candidate for fish vaccination via an immersion administering route. Article in Journal/Newspaper Scophthalmus maximus Turbot Directory of Open Access Journals: DOAJ Articles International Journal of Molecular Sciences 17 3 325 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Quillaja saponaria saponins adjuvant vaccination Scophthalmus maximus relative percent of survival (RPS) Biology (General) QH301-705.5 Chemistry QD1-999 |
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Quillaja saponaria saponins adjuvant vaccination Scophthalmus maximus relative percent of survival (RPS) Biology (General) QH301-705.5 Chemistry QD1-999 Yujuan Wang Xiuhua Wang Jie Huang Jun Li Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination |
topic_facet |
Quillaja saponaria saponins adjuvant vaccination Scophthalmus maximus relative percent of survival (RPS) Biology (General) QH301-705.5 Chemistry QD1-999 |
description |
The adjuvant effect of Quillaja saponaria saponin (QSS) on protection of turbot fry was investigated with immersion vaccination of formalin-killed Vibrio anguillarum O1 and various concentrations of QSS (5, 25, 45 and 65 mg/L). Fish were challenged at days 7, 14 and 28 post-vaccination. Significantly high relative percent of survival (RPS) ((59.1 ± 13.6)%, (81.7 ± 8.2)%, (77.8 ± 9.6)%) were recorded in the fish that received bacterins immersion with QSS at 45 mg/L, which is comparable to the positive control group vaccinated by intraperitoneal injection (IP). Moreover, a remarkably higher serum antibody titer was also demonstrated after 28 days in the vaccinated fish with QSS (45 mg/L) than those vaccinated fish without QSS (p < 0.05), but lower than the IP immunized fish (p < 0.05). Significant upregulation of IgM gene expression has also been identified in the tissues of skin, gill, spleen and kidney from the immunized fish in comparison to the control fish. Taken together, the present study indicated that QSS was able to dramatically evoke systemic and mucosal immune responses in immunized fish. Therefore, QSS might be a promising adjuvant candidate for fish vaccination via an immersion administering route. |
format |
Article in Journal/Newspaper |
author |
Yujuan Wang Xiuhua Wang Jie Huang Jun Li |
author_facet |
Yujuan Wang Xiuhua Wang Jie Huang Jun Li |
author_sort |
Yujuan Wang |
title |
Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination |
title_short |
Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination |
title_full |
Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination |
title_fullStr |
Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination |
title_full_unstemmed |
Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination |
title_sort |
adjuvant effect of quillaja saponaria saponin (qss) on protective efficacy and igm generation in turbot (scophthalmus maximus) upon immersion vaccination |
publisher |
MDPI AG |
publishDate |
2016 |
url |
https://doi.org/10.3390/ijms17030325 https://doaj.org/article/ad1acd5bf97b4d34b0fb39038159cd4f |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
International Journal of Molecular Sciences, Vol 17, Iss 3, p 325 (2016) |
op_relation |
http://www.mdpi.com/1422-0067/17/3/325 https://doaj.org/toc/1422-0067 1422-0067 doi:10.3390/ijms17030325 https://doaj.org/article/ad1acd5bf97b4d34b0fb39038159cd4f |
op_doi |
https://doi.org/10.3390/ijms17030325 |
container_title |
International Journal of Molecular Sciences |
container_volume |
17 |
container_issue |
3 |
container_start_page |
325 |
_version_ |
1766188925637885952 |