Comparative Genomics Analysis of Vibrio anguillarum Isolated from Lumpfish ( Cyclopterus lumpus ) in Newfoundland Reveal Novel Chromosomal Organizations
Vibrio anguillarum is a Gram-negative marine pathogen causative agent of vibriosis in a wide range of hosts, including invertebrates and teleosts. Lumpfish ( Cyclopterus lumpus ), a native fish of the North Atlantic Ocean, is utilized as cleaner fish to control sea lice ( Lepeophtheirus salmonis ) i...
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ftdoajarticles:oai:doaj.org/article:aceae1e943d54f9ba7b5a4f19fb39614 2023-05-15T15:32:35+02:00 Comparative Genomics Analysis of Vibrio anguillarum Isolated from Lumpfish ( Cyclopterus lumpus ) in Newfoundland Reveal Novel Chromosomal Organizations Ignacio Vasquez Trung Cao Setu Chakraborty Hajarooba Gnanagobal Nicole O’Brien Jennifer Monk Danny Boyce Jillian D. Westcott Javier Santander 2020-10-01T00:00:00Z https://doi.org/10.3390/microorganisms8111666 https://doaj.org/article/aceae1e943d54f9ba7b5a4f19fb39614 EN eng MDPI AG https://www.mdpi.com/2076-2607/8/11/1666 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms8111666 2076-2607 https://doaj.org/article/aceae1e943d54f9ba7b5a4f19fb39614 Microorganisms, Vol 8, Iss 1666, p 1666 (2020) Vibrio anguillarum lumpfish genomics insertion elements evolution Biology (General) QH301-705.5 article 2020 ftdoajarticles https://doi.org/10.3390/microorganisms8111666 2022-12-31T01:19:58Z Vibrio anguillarum is a Gram-negative marine pathogen causative agent of vibriosis in a wide range of hosts, including invertebrates and teleosts. Lumpfish ( Cyclopterus lumpus ), a native fish of the North Atlantic Ocean, is utilized as cleaner fish to control sea lice ( Lepeophtheirus salmonis ) infestations in the Atlantic salmon ( Salmo salar ) aquaculture industry. V. anguillarum is one of the most frequent bacterial pathogens affecting lumpfish. Here, we described the phenotype and genomic characteristics of V. anguillarum strain J360 isolated from infected cultured lumpfish in Newfoundland, Canada. Koch’s postulates determined in naïve lumpfish showed lethal acute vibriosis in lumpfish. The V. anguillarum J360 genome was shown to be composed of two chromosomes and two plasmids with a total genome size of 4.56 Mb with 44.85% G + C content. Phylogenetic and comparative analyses showed that V. anguillarum J360 is closely related to V. anguillarum strain VIB43, isolated in Scotland, with a 99.8% genome identity. Differences in the genomic organization were identified and associated with insertion sequence elements (ISs). Additionally, V. anguillarum J360 does not possess a pJM1-like plasmid, typically present in virulent isolates from the Pacific Ocean, suggesting that acquisition of this extrachromosomal element and the virulence of V. anguillarum J360 or other Atlantic isolates could increase. Article in Journal/Newspaper Atlantic salmon Newfoundland North Atlantic Salmo salar Directory of Open Access Journals: DOAJ Articles Canada Pacific Microorganisms 8 11 1666 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Vibrio anguillarum lumpfish genomics insertion elements evolution Biology (General) QH301-705.5 |
spellingShingle |
Vibrio anguillarum lumpfish genomics insertion elements evolution Biology (General) QH301-705.5 Ignacio Vasquez Trung Cao Setu Chakraborty Hajarooba Gnanagobal Nicole O’Brien Jennifer Monk Danny Boyce Jillian D. Westcott Javier Santander Comparative Genomics Analysis of Vibrio anguillarum Isolated from Lumpfish ( Cyclopterus lumpus ) in Newfoundland Reveal Novel Chromosomal Organizations |
topic_facet |
Vibrio anguillarum lumpfish genomics insertion elements evolution Biology (General) QH301-705.5 |
description |
Vibrio anguillarum is a Gram-negative marine pathogen causative agent of vibriosis in a wide range of hosts, including invertebrates and teleosts. Lumpfish ( Cyclopterus lumpus ), a native fish of the North Atlantic Ocean, is utilized as cleaner fish to control sea lice ( Lepeophtheirus salmonis ) infestations in the Atlantic salmon ( Salmo salar ) aquaculture industry. V. anguillarum is one of the most frequent bacterial pathogens affecting lumpfish. Here, we described the phenotype and genomic characteristics of V. anguillarum strain J360 isolated from infected cultured lumpfish in Newfoundland, Canada. Koch’s postulates determined in naïve lumpfish showed lethal acute vibriosis in lumpfish. The V. anguillarum J360 genome was shown to be composed of two chromosomes and two plasmids with a total genome size of 4.56 Mb with 44.85% G + C content. Phylogenetic and comparative analyses showed that V. anguillarum J360 is closely related to V. anguillarum strain VIB43, isolated in Scotland, with a 99.8% genome identity. Differences in the genomic organization were identified and associated with insertion sequence elements (ISs). Additionally, V. anguillarum J360 does not possess a pJM1-like plasmid, typically present in virulent isolates from the Pacific Ocean, suggesting that acquisition of this extrachromosomal element and the virulence of V. anguillarum J360 or other Atlantic isolates could increase. |
format |
Article in Journal/Newspaper |
author |
Ignacio Vasquez Trung Cao Setu Chakraborty Hajarooba Gnanagobal Nicole O’Brien Jennifer Monk Danny Boyce Jillian D. Westcott Javier Santander |
author_facet |
Ignacio Vasquez Trung Cao Setu Chakraborty Hajarooba Gnanagobal Nicole O’Brien Jennifer Monk Danny Boyce Jillian D. Westcott Javier Santander |
author_sort |
Ignacio Vasquez |
title |
Comparative Genomics Analysis of Vibrio anguillarum Isolated from Lumpfish ( Cyclopterus lumpus ) in Newfoundland Reveal Novel Chromosomal Organizations |
title_short |
Comparative Genomics Analysis of Vibrio anguillarum Isolated from Lumpfish ( Cyclopterus lumpus ) in Newfoundland Reveal Novel Chromosomal Organizations |
title_full |
Comparative Genomics Analysis of Vibrio anguillarum Isolated from Lumpfish ( Cyclopterus lumpus ) in Newfoundland Reveal Novel Chromosomal Organizations |
title_fullStr |
Comparative Genomics Analysis of Vibrio anguillarum Isolated from Lumpfish ( Cyclopterus lumpus ) in Newfoundland Reveal Novel Chromosomal Organizations |
title_full_unstemmed |
Comparative Genomics Analysis of Vibrio anguillarum Isolated from Lumpfish ( Cyclopterus lumpus ) in Newfoundland Reveal Novel Chromosomal Organizations |
title_sort |
comparative genomics analysis of vibrio anguillarum isolated from lumpfish ( cyclopterus lumpus ) in newfoundland reveal novel chromosomal organizations |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/microorganisms8111666 https://doaj.org/article/aceae1e943d54f9ba7b5a4f19fb39614 |
geographic |
Canada Pacific |
geographic_facet |
Canada Pacific |
genre |
Atlantic salmon Newfoundland North Atlantic Salmo salar |
genre_facet |
Atlantic salmon Newfoundland North Atlantic Salmo salar |
op_source |
Microorganisms, Vol 8, Iss 1666, p 1666 (2020) |
op_relation |
https://www.mdpi.com/2076-2607/8/11/1666 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms8111666 2076-2607 https://doaj.org/article/aceae1e943d54f9ba7b5a4f19fb39614 |
op_doi |
https://doi.org/10.3390/microorganisms8111666 |
container_title |
Microorganisms |
container_volume |
8 |
container_issue |
11 |
container_start_page |
1666 |
_version_ |
1766363071924666368 |