Arsenate resistance in the unicellular marine diazotroph Crocosphaera watsonii
The toxic arsenate ion can behave as a phosphate analog, and this can result in arsenate toxicity especially in areas with elevated arsenate to phosphate ratios like the surface waters of the ocean gyres. In these systems, cellular arsenate resistance strategies would allow phytoplankton to ameliora...
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ftdoajarticles:oai:doaj.org/article:acb8789518ab444d8e308059e6de4622 2023-05-15T17:35:35+02:00 Arsenate resistance in the unicellular marine diazotroph Crocosphaera watsonii Sonya eDyhrman Sheean eHaley 2011-10-01T00:00:00Z https://doi.org/10.3389/fmicb.2011.00214 https://doaj.org/article/acb8789518ab444d8e308059e6de4622 EN eng Frontiers Media S.A. http://journal.frontiersin.org/Journal/10.3389/fmicb.2011.00214/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2011.00214 https://doaj.org/article/acb8789518ab444d8e308059e6de4622 Frontiers in Microbiology, Vol 2 (2011) Cyanobacteria Gene Expression Phosphorus arsenate marine Crocosphaera Microbiology QR1-502 article 2011 ftdoajarticles https://doi.org/10.3389/fmicb.2011.00214 2022-12-31T03:02:59Z The toxic arsenate ion can behave as a phosphate analog, and this can result in arsenate toxicity especially in areas with elevated arsenate to phosphate ratios like the surface waters of the ocean gyres. In these systems, cellular arsenate resistance strategies would allow phytoplankton to ameliorate the effects of arsenate transport into the cell. Despite the potential coupling between arsenate and phosphate cycling in oligotrophic marine waters, relatively little is known about arsenate resistance in the nitrogen-fixing marine cyanobacteria that are key components of the microbial community in low nutrient systems. The unicellular diazotroph, Crocosphaera watsonii WH8501, was able to grow at reduced rates with arsenate additions up to 30 nM, and estimated arsenate to phosphate ratios of 6:1. The genome of strain WH8501 contains homologs for arsA, arsH, arsB and arsC, allowing for the reduction of arsenate to arsenite and the pumping of arsenite out of the cell. The short-term addition of arsenate to the growth medium had no effect on nitrogen fixation. However, arsenate addition did result in the up-regulation of the arsB gene with increasing arsenate concentrations, indicating the induction of the arsenate detoxification response. The arsB gene was also up-regulated by phosphorus stress in concert with a gene encoding the high-affinity phosphate binding protein pstS. Both genes were down-regulated when phosphate was re-fed to phosphorus-stressed cells. A field survey of surface water from the low phosphate western North Atlantic detected expression of C. watsonii arsB, suggestive of the potential importance of arsenate resistance strategies in this and perhaps other systems. Article in Journal/Newspaper North Atlantic Directory of Open Access Journals: DOAJ Articles Frontiers in Microbiology 2 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Cyanobacteria Gene Expression Phosphorus arsenate marine Crocosphaera Microbiology QR1-502 |
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Cyanobacteria Gene Expression Phosphorus arsenate marine Crocosphaera Microbiology QR1-502 Sonya eDyhrman Sheean eHaley Arsenate resistance in the unicellular marine diazotroph Crocosphaera watsonii |
topic_facet |
Cyanobacteria Gene Expression Phosphorus arsenate marine Crocosphaera Microbiology QR1-502 |
description |
The toxic arsenate ion can behave as a phosphate analog, and this can result in arsenate toxicity especially in areas with elevated arsenate to phosphate ratios like the surface waters of the ocean gyres. In these systems, cellular arsenate resistance strategies would allow phytoplankton to ameliorate the effects of arsenate transport into the cell. Despite the potential coupling between arsenate and phosphate cycling in oligotrophic marine waters, relatively little is known about arsenate resistance in the nitrogen-fixing marine cyanobacteria that are key components of the microbial community in low nutrient systems. The unicellular diazotroph, Crocosphaera watsonii WH8501, was able to grow at reduced rates with arsenate additions up to 30 nM, and estimated arsenate to phosphate ratios of 6:1. The genome of strain WH8501 contains homologs for arsA, arsH, arsB and arsC, allowing for the reduction of arsenate to arsenite and the pumping of arsenite out of the cell. The short-term addition of arsenate to the growth medium had no effect on nitrogen fixation. However, arsenate addition did result in the up-regulation of the arsB gene with increasing arsenate concentrations, indicating the induction of the arsenate detoxification response. The arsB gene was also up-regulated by phosphorus stress in concert with a gene encoding the high-affinity phosphate binding protein pstS. Both genes were down-regulated when phosphate was re-fed to phosphorus-stressed cells. A field survey of surface water from the low phosphate western North Atlantic detected expression of C. watsonii arsB, suggestive of the potential importance of arsenate resistance strategies in this and perhaps other systems. |
format |
Article in Journal/Newspaper |
author |
Sonya eDyhrman Sheean eHaley |
author_facet |
Sonya eDyhrman Sheean eHaley |
author_sort |
Sonya eDyhrman |
title |
Arsenate resistance in the unicellular marine diazotroph Crocosphaera watsonii |
title_short |
Arsenate resistance in the unicellular marine diazotroph Crocosphaera watsonii |
title_full |
Arsenate resistance in the unicellular marine diazotroph Crocosphaera watsonii |
title_fullStr |
Arsenate resistance in the unicellular marine diazotroph Crocosphaera watsonii |
title_full_unstemmed |
Arsenate resistance in the unicellular marine diazotroph Crocosphaera watsonii |
title_sort |
arsenate resistance in the unicellular marine diazotroph crocosphaera watsonii |
publisher |
Frontiers Media S.A. |
publishDate |
2011 |
url |
https://doi.org/10.3389/fmicb.2011.00214 https://doaj.org/article/acb8789518ab444d8e308059e6de4622 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Frontiers in Microbiology, Vol 2 (2011) |
op_relation |
http://journal.frontiersin.org/Journal/10.3389/fmicb.2011.00214/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2011.00214 https://doaj.org/article/acb8789518ab444d8e308059e6de4622 |
op_doi |
https://doi.org/10.3389/fmicb.2011.00214 |
container_title |
Frontiers in Microbiology |
container_volume |
2 |
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1766134788147642368 |