Hemophagocytosis induced by Leishmania donovani infection is beneficial to parasite survival within macrophages.
Visceral leishmaniasis (VL) is caused by parasitic protozoa of the genus Leishmania and is characterized by clinical manifestations such as fever, hepatosplenomegaly and anemia. Hemophagocytosis, the phenomenon of phagocytosis of blood cells by macrophages, is found in VL patients. In a previous stu...
Published in: | PLOS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2019
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0007816 https://doaj.org/article/ac86b0ad717c43b98fdd2cfffb013099 |
id |
ftdoajarticles:oai:doaj.org/article:ac86b0ad717c43b98fdd2cfffb013099 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:ac86b0ad717c43b98fdd2cfffb013099 2023-05-15T15:10:06+02:00 Hemophagocytosis induced by Leishmania donovani infection is beneficial to parasite survival within macrophages. Ayako Morimoto Kazuyuki Uchida James K Chambers Kai Sato Jing Hong Chizu Sanjoba Yoshitsugu Matsumoto Junya Yamagishi Yasuyuki Goto 2019-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007816 https://doaj.org/article/ac86b0ad717c43b98fdd2cfffb013099 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007816 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007816 https://doaj.org/article/ac86b0ad717c43b98fdd2cfffb013099 PLoS Neglected Tropical Diseases, Vol 13, Iss 11, p e0007816 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007816 2022-12-31T10:07:30Z Visceral leishmaniasis (VL) is caused by parasitic protozoa of the genus Leishmania and is characterized by clinical manifestations such as fever, hepatosplenomegaly and anemia. Hemophagocytosis, the phenomenon of phagocytosis of blood cells by macrophages, is found in VL patients. In a previous study we established an experimental model of VL, reproducing anemia in mice for the first time, and identified hemophagocytosis by heavily infected macrophages in the spleen as a possible cause of anemia. However, the mechanism for parasite-induced hemophagocytosis or its role in parasite survival remained unclear. Here, we established an in vitro model of Leishmania-induced hemophagocytosis to explore the molecules involved in this process. In contrast to naïve RAW264.7 cells (mouse macrophage cell line) which did not uptake freshly isolated erythrocytes, RAW264.7 cells infected with L. donovani showed enhanced phagocytosis of erythrocytes. Additionally, for hemophagocytes found both in vitro and in vivo, the expression of signal regulatory protein α (SIRPα), one of the receptors responsible for the 'don't-eat-me' signal was suppressed by post-transcriptional control. Furthermore, the overlapped phagocytosis of erythrocytes and Leishmania parasites within a given macrophage appeared to be beneficial to the parasites; the in vitro experiments showed a higher number of parasites within macrophages that had been induced to engulf erythrocytes. Together, these results suggest that Leishmania parasites may actively induce hemophagocytosis by manipulating the expression of SIRPα in macrophages/hemophagocytes, in order to secure their parasitism. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 13 11 e0007816 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Ayako Morimoto Kazuyuki Uchida James K Chambers Kai Sato Jing Hong Chizu Sanjoba Yoshitsugu Matsumoto Junya Yamagishi Yasuyuki Goto Hemophagocytosis induced by Leishmania donovani infection is beneficial to parasite survival within macrophages. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Visceral leishmaniasis (VL) is caused by parasitic protozoa of the genus Leishmania and is characterized by clinical manifestations such as fever, hepatosplenomegaly and anemia. Hemophagocytosis, the phenomenon of phagocytosis of blood cells by macrophages, is found in VL patients. In a previous study we established an experimental model of VL, reproducing anemia in mice for the first time, and identified hemophagocytosis by heavily infected macrophages in the spleen as a possible cause of anemia. However, the mechanism for parasite-induced hemophagocytosis or its role in parasite survival remained unclear. Here, we established an in vitro model of Leishmania-induced hemophagocytosis to explore the molecules involved in this process. In contrast to naïve RAW264.7 cells (mouse macrophage cell line) which did not uptake freshly isolated erythrocytes, RAW264.7 cells infected with L. donovani showed enhanced phagocytosis of erythrocytes. Additionally, for hemophagocytes found both in vitro and in vivo, the expression of signal regulatory protein α (SIRPα), one of the receptors responsible for the 'don't-eat-me' signal was suppressed by post-transcriptional control. Furthermore, the overlapped phagocytosis of erythrocytes and Leishmania parasites within a given macrophage appeared to be beneficial to the parasites; the in vitro experiments showed a higher number of parasites within macrophages that had been induced to engulf erythrocytes. Together, these results suggest that Leishmania parasites may actively induce hemophagocytosis by manipulating the expression of SIRPα in macrophages/hemophagocytes, in order to secure their parasitism. |
format |
Article in Journal/Newspaper |
author |
Ayako Morimoto Kazuyuki Uchida James K Chambers Kai Sato Jing Hong Chizu Sanjoba Yoshitsugu Matsumoto Junya Yamagishi Yasuyuki Goto |
author_facet |
Ayako Morimoto Kazuyuki Uchida James K Chambers Kai Sato Jing Hong Chizu Sanjoba Yoshitsugu Matsumoto Junya Yamagishi Yasuyuki Goto |
author_sort |
Ayako Morimoto |
title |
Hemophagocytosis induced by Leishmania donovani infection is beneficial to parasite survival within macrophages. |
title_short |
Hemophagocytosis induced by Leishmania donovani infection is beneficial to parasite survival within macrophages. |
title_full |
Hemophagocytosis induced by Leishmania donovani infection is beneficial to parasite survival within macrophages. |
title_fullStr |
Hemophagocytosis induced by Leishmania donovani infection is beneficial to parasite survival within macrophages. |
title_full_unstemmed |
Hemophagocytosis induced by Leishmania donovani infection is beneficial to parasite survival within macrophages. |
title_sort |
hemophagocytosis induced by leishmania donovani infection is beneficial to parasite survival within macrophages. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007816 https://doaj.org/article/ac86b0ad717c43b98fdd2cfffb013099 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 11, p e0007816 (2019) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007816 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007816 https://doaj.org/article/ac86b0ad717c43b98fdd2cfffb013099 |
op_doi |
https://doi.org/10.1371/journal.pntd.0007816 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
13 |
container_issue |
11 |
container_start_page |
e0007816 |
_version_ |
1766341162116841472 |