Improvement of a tissue maceration technique for the determination of placental involvement in schistosomiasis.
Schistosomiasis in pregnancy may cause low birth weight, prematurity and stillbirth of the offspring. The placenta of pregnant women might be involved when schistosome ova are trapped in placental tissue. Standard histopathological methods only allow the examination of a limited amount of placental...
Published in: | PLOS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2017
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0005551 https://doaj.org/article/ac84a6ed295449fea3b1dbed345f5446 |
id |
ftdoajarticles:oai:doaj.org/article:ac84a6ed295449fea3b1dbed345f5446 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:ac84a6ed295449fea3b1dbed345f5446 2023-05-15T15:10:01+02:00 Improvement of a tissue maceration technique for the determination of placental involvement in schistosomiasis. Martha Charlotte Holtfreter Heinrich Neubauer Tanja Groten Hosny El-Adawy Jana Pastuschek Joachim Richter Dieter Häussinger Mathias Wilhelm Pletz Benjamin Thomas Schleenvoigt 2017-04-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005551 https://doaj.org/article/ac84a6ed295449fea3b1dbed345f5446 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5402960?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005551 https://doaj.org/article/ac84a6ed295449fea3b1dbed345f5446 PLoS Neglected Tropical Diseases, Vol 11, Iss 4, p e0005551 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005551 2022-12-30T21:04:39Z Schistosomiasis in pregnancy may cause low birth weight, prematurity and stillbirth of the offspring. The placenta of pregnant women might be involved when schistosome ova are trapped in placental tissue. Standard histopathological methods only allow the examination of a limited amount of placental tissue and are therefore not sufficiently sensitive. Thus, placental schistosomiasis remains underdiagnosed and its role in contributing to schistosomiasis-associated pregnancy outcomes remains unclear. Here we investigated an advanced maceration method in order to recover a maximum number of schistosome ova from the placenta. We examined the effect of different potassium hydroxide (KOH) concentrations and different tissue fixatives with respect to maceration success and egg morphology. Placental tissue was kept either in 0.9% saline, 5% formalin or 70% ethanol and was macerated together with Schistosoma mansoni infested mouse livers and KOH 4% or 10%, respectively. We found that placenta maceration using 4% KOH at 37°C for 24 h was the most effective method: placental tissue was completely digested, egg morphology was well preserved and alkaline concentration was the lowest. Ethanol proved to be the best fixative for this method. Here we propose an improved maceration technique in terms of sensitivity, safety and required skills, which may enable its wider use also in endemic areas. This technique may contribute to clarifying the role of placental involvement in pregnant women with schistosomiasis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 4 e0005551 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Martha Charlotte Holtfreter Heinrich Neubauer Tanja Groten Hosny El-Adawy Jana Pastuschek Joachim Richter Dieter Häussinger Mathias Wilhelm Pletz Benjamin Thomas Schleenvoigt Improvement of a tissue maceration technique for the determination of placental involvement in schistosomiasis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Schistosomiasis in pregnancy may cause low birth weight, prematurity and stillbirth of the offspring. The placenta of pregnant women might be involved when schistosome ova are trapped in placental tissue. Standard histopathological methods only allow the examination of a limited amount of placental tissue and are therefore not sufficiently sensitive. Thus, placental schistosomiasis remains underdiagnosed and its role in contributing to schistosomiasis-associated pregnancy outcomes remains unclear. Here we investigated an advanced maceration method in order to recover a maximum number of schistosome ova from the placenta. We examined the effect of different potassium hydroxide (KOH) concentrations and different tissue fixatives with respect to maceration success and egg morphology. Placental tissue was kept either in 0.9% saline, 5% formalin or 70% ethanol and was macerated together with Schistosoma mansoni infested mouse livers and KOH 4% or 10%, respectively. We found that placenta maceration using 4% KOH at 37°C for 24 h was the most effective method: placental tissue was completely digested, egg morphology was well preserved and alkaline concentration was the lowest. Ethanol proved to be the best fixative for this method. Here we propose an improved maceration technique in terms of sensitivity, safety and required skills, which may enable its wider use also in endemic areas. This technique may contribute to clarifying the role of placental involvement in pregnant women with schistosomiasis. |
format |
Article in Journal/Newspaper |
author |
Martha Charlotte Holtfreter Heinrich Neubauer Tanja Groten Hosny El-Adawy Jana Pastuschek Joachim Richter Dieter Häussinger Mathias Wilhelm Pletz Benjamin Thomas Schleenvoigt |
author_facet |
Martha Charlotte Holtfreter Heinrich Neubauer Tanja Groten Hosny El-Adawy Jana Pastuschek Joachim Richter Dieter Häussinger Mathias Wilhelm Pletz Benjamin Thomas Schleenvoigt |
author_sort |
Martha Charlotte Holtfreter |
title |
Improvement of a tissue maceration technique for the determination of placental involvement in schistosomiasis. |
title_short |
Improvement of a tissue maceration technique for the determination of placental involvement in schistosomiasis. |
title_full |
Improvement of a tissue maceration technique for the determination of placental involvement in schistosomiasis. |
title_fullStr |
Improvement of a tissue maceration technique for the determination of placental involvement in schistosomiasis. |
title_full_unstemmed |
Improvement of a tissue maceration technique for the determination of placental involvement in schistosomiasis. |
title_sort |
improvement of a tissue maceration technique for the determination of placental involvement in schistosomiasis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005551 https://doaj.org/article/ac84a6ed295449fea3b1dbed345f5446 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 4, p e0005551 (2017) |
op_relation |
http://europepmc.org/articles/PMC5402960?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005551 https://doaj.org/article/ac84a6ed295449fea3b1dbed345f5446 |
op_doi |
https://doi.org/10.1371/journal.pntd.0005551 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
4 |
container_start_page |
e0005551 |
_version_ |
1766341095627685888 |